Limonene inhibits Candida albicans growth by inducing apoptosis

Thakre, Archana; Zore, Gajanan; Kodgire, Santosh; Kazi, Rubina; Mulange, Shradha V.; Patil, Rajendra; Shelar, Amruta; Santhakumari, B.; Kulkarni, Mahesh J.; Kharat, Kiran; Karuppayil, Sankunny Mohan

doi:10.1093/mmy/myx074

Cited by 46 publications

(41 citation statements)

References 67 publications

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“…Previous studies described that the use of this monoterpene in concentrations between 0.6 to 5 mM induces apoptosis in C. albicans , due to the damage caused to both the cell wall and membrane. In that study, the authors claimed that cell death was likely due to oxidative stress produced by limonene, which leads to DNA damage, alterations of the cell cycle, and, finally, yeast apoptosis [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Inhibitory Activity of Limonene against Different Isolates of Candida spp.

Muñoz

Rossi

Jabès

et al. 2020

JoF

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Commensal yeast from the genus Candida is part of the healthy human microbiota. In some cases, Candida spp. dysbiosis can result in candidiasis, the symptoms of which may vary from mild localized rashes to severe disseminated infections. The most prevalent treatments against candidiasis involve fluconazole, itraconazole, miconazole, and caspofungin. Moreover, amphotericin B associated with prolonged azole administration is utilized to control severe cases. Currently, numerous guidelines recommend echinocandins to treat invasive candidiasis. However, resistance to these antifungal drugs has increased dramatically over recent years. Considering this situation, new therapeutic alternatives should be studied to control candidiasis, which has become a major medical concern. Limonene belongs to the group of terpene molecules, known for their pharmacological properties. In this study, we evaluated in vitro the limonene concentration capable of inhibiting the growth of yeast from the genus Candida susceptible or resistant to antifungal drugs and its capacity to induce fungal damage. In addition, intravaginal fungal infection assays using a murine model infected by Candida albicans were carried out and the fungal burden, histopathology, and scanning electron microscopy were evaluated. All of our results suggest that limonene may play a protective role against the infection process by yeast from the genus Candida.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Inhibitory Activity of Limonene against Different Isolates of Candida spp.

Muñoz

Rossi

Jabès

et al. 2020

JoF

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“…Limonene damages the C. albicans cell wall/membrane, thereby modifying cellular adhesion and plasticity, pH, and ionic content [26]. In addition, such damage causes oxidative stress and consequent DNA damage, resulting in cell cycle modulation and apoptosis as demonstrated by Thakre et al [28]. The high concentration of limonene in the EO could contribute to its nematocidal effect [29].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Rds

Andrade

et al. 2019

Plants

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Candidiasis therapy, especially for candidiasis caused by Candida non-albicans species, is limited by the relatively reduced number of antifungal drugs and the emergence of antifungal tolerance. This study evaluates the anticandidal activity of 41 plant-derived products against Candida species, in both planktonic and biofilm cells. This study also evaluates the toxicity and the therapeutic action of the most active compounds by using the Caenorhabditis elegans–Candida model. The planktonic cells were cultured with various concentrations of the tested agents. The Cupressus sempervirens, Citrus limon, and Litsea cubeba essential oils as well as gallic acid were the most active anticandidal compounds. Candida cell re-growth after treatment with these agents for 48 h demonstrated that the L. cubeba essential oil and gallic acid displayed fungistatic activity, whereas the C. limon and C. sempervirens essential oils exhibited fungicidal activity. The C. sempervirens essential oil was not toxic and increased the survival of C. elegans worms infected with C. glabrata or C. orthopsilosis. All the plant-derived products assayed at 250 µg/mL affected C. krusei biofilms. The tested plant-derived products proved to be potential therapeutic agents against Candida, especially Candida non-albicans species, and should be considered when developing new anticandidal agents.

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“…The activity of this EO would be related to the large amount of limonene, sabinene and citronellal. These compounds strongly inhibit C. albicans [22,23,24]. However, S. cerevisiae showed a reduced sensitivity versus other EOs provided of antifungal activity, such as L. cubeba and C. citratus.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Activity of Essential Oils against Salmonella enterica Serotypes Enteritidis and Typhimurium Strains Isolated from Poultry

et al. 2019

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Salmonella enterica serotype Enteritidis and S. enterica serotype Typhimurium are frequently present among poultry and are associated with outbreaks of human salmonellosis. The study investigated the in vitro antimicrobial activity of essential oils (EOs) obtained from Aloysia triphylla, Cinnamomum zeylanicum, Cymbopogon citratus, Litsea cubeba, Mentha piperita, Syzygium aromaticum against S. Enteritidis and S. Thyphimurium strains previously isolated from poultry. A 1:1 mixture of C. zeylanicum and S. aromaticum was also tested. The activity of all compounds was evaluated against the yeast Saccharomyces cerevisiae, commonly used as probiotic. The highest antibacterial activity was observed for C. zeylanicum (minimum inhibitory concentrations (MICs) ranging from 1.26 mg/mL to 0.63 mg/mL), S. aromaticum (MICs from 2.637 mg/mL to 0.164 mg/mL) and the mixture (MICs from 1.289 mg/mL to 0.322 mg/mL). No activity was recorded against S. cerevisiae. The results suggest a possible use of C. zeylanicum and S. aromaticum, alone or in combination, in the farm environment for disinfection and in poultry diet, combined with S. cerevisiae administration, for an integrated approach to avoid Salmonella intestinal colonization.

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Limonene inhibits Candida albicans growth by inducing apoptosis

Cited by 46 publications

References 67 publications

In Vitro and In Vivo Inhibitory Activity of Limonene against Different Isolates of Candida spp.

In Vitro and In Vivo Inhibitory Activity of Limonene against Different Isolates of Candida spp.

In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

In Vitro Antimicrobial Activity of Essential Oils against Salmonella enterica Serotypes Enteritidis and Typhimurium Strains Isolated from Poultry

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