In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Rds, Pedroso; Bl, Balbino; Andrade, Géssica; Mcps, Dias; Ta, Alvarenga; Rcn, Pedroso; Lp, Pimenta; Lucarini, Rodrigo; Pauletti, Patrícia Mendonça; Januário, AH; Mtm, Carvalho; Miranda, Mayker Lazaro Dantas; Pires, Regina Helena

doi:10.3390/plants8110494

Cited by 33 publications

(54 citation statements)

References 43 publications

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“…Based on in vitro and in vivo data, echinocandins and amphotericin B solutions are the most promising combination-based and/or antifungal lock strategies [ 19 ]. Further innovative therapeutic approaches may be the natural products-based treatments [ 22 , 23 ]. One of the more well-studied compounds is carbohydrate-derived fulvic acid as a heat stable colloidal material, which has an inhibitory effect on Candida and bacterial biofilm formation [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Kovács

Majoros

2020

JoF

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The number of effective therapeutic strategies against biofilms is limited; development of novel therapies is urgently needed to treat a variety of biofilm-associated infections. Quorum sensing is a special form of microbial cell-to-cell communication that is responsible for the release of numerous extracellular molecules, whose concentration is proportional with cell density. Candida-secreted quorum-sensing molecules (i.e., farnesol and tyrosol) have a pivotal role in morphogenesis, biofilm formation, and virulence. Farnesol can mediate the hyphae-to-yeast transition, while tyrosol has the opposite effect of inducing transition from the yeast to hyphal form. A number of questions regarding Candida quorum sensing remain to be addressed; nevertheless, the literature shows that farnesol and tyrosol possess remarkable antifungal and anti-biofilm effect at supraphysiological concentration. Furthermore, previous in vitro and in vivo data suggest that they may have a potent adjuvant effect in combination with certain traditional antifungal agents. This review discusses the most promising farnesol- and tyrosol-based in vitro and in vivo results, which may be a foundation for future development of novel therapeutic strategies to combat Candida biofilms.

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Section: Introductionmentioning

confidence: 99%

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Kovács

Majoros

2020

JoF

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“…Studies have shown that LCEO is a cell wall-active agent and can cause cell membrane damage of S. aureus [ 11 ], and our current results were similar. S. aureus is a Gram-positive bacteria and A. baumannii 17 is a Gram-negative bacteria, which again showed that LCEO has spectral antimicrobial activity [ 7 , 53 , 54 , 55 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Yang

Hao

Jiang

et al. 2021

Genes

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Litsea cubeba L. essential oil (LCEO) can affect the growth of drug-resistance bacteria. However, research on stress response of drug-resistant A. baumannii under sub-lethal LCEO concentrations had been limited so far. Therefore, transcriptomic analysis of A. baumannii under 1/2 minimum inhibitory concentration (MIC, 0.54 mg/mL) of LCEO was performed. Results of transcriptomic analysis showed that 320/352 genes were significantly up/down-regulated, respectively, in LCEO-treated A. baumannii. Both up and down-regulated genes were significantly enriched in three GO terms (oxidation-reduction process; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors), which indicated that the redox state of A. baumannii was significantly affected by LCEO. LCEO may also inhibit aerobic respiration, synthesis of ketone bodies and the metabolism of some amino acids while, meanwhile, promoting fatty acid degradation of A. baumannii according to Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. The permeability and the stress of cell membrane of A. baumannii were significantly affected by LCEO. After crystal violet dyeing, the biofilm formation of A. baumannii was promoted/inhibited by extremely low/relatively high concentration of LCEO, respectively. LCEO and chloramphenicol have synergistic growth inhibitory effect against A. baumannii according to the Fractional Inhibitory Concentration Index (FICI) value = 0.375. Our results indicate that the growth of A. baumannii was inhibited by LCEO, and give insights into the stress response of A. baumannii under sub-lethal concentrations of LCEO. These results provided evidence that A. baumannii was inhibited by LCEO, and expanded knowledges of stress response of A. baumannii under sub-lethal concentration of LCEO.

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“…elegans has been extensively used for studying several dimorphic fungi of clinical relevance. The most devastating and pathogenic dimorphic fungus that has been adequately explored with this nematode model is Candida albicans (Hans et al, 2019a;Hans et al, 2019b;Song et al, 2019;Venkata et al, 2020) and a few other non-albicans species such as C. tropicalis (Brilhante et al, 2016;Feistel et al, 2019;Pedroso et al, 2019), C. krusei (De Aguiar Cordeiro et al, 2018Kunyeit et al, 2019), and C. auris (Eldesouky et al, 2018a;Mohammad et al, 2019). Another important clinical dimorphic fungus, Taloromyces (Penicillium) marneffei, has also been studied in a C. elegans model for both virulence tests and antifungal agent efficacy evaluations (Huang et al, 2014;Sangkanu et al, 2021).…”

Section: Application Of C Elegans For Dimorphic Fungi Studiesmentioning

confidence: 99%

Caenorhabditis elegans as an Infection Model for Pathogenic Mold and Dimorphic Fungi: Applications and Challenges

Ahamefule

Ezeuduji

Ogbonna

et al. 2021

Front. Cell. Infect. Microbiol.

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The threat burden from pathogenic fungi is universal and increasing with alarming high mortality and morbidity rates from invasive fungal infections. Understanding the virulence factors of these fungi, screening effective antifungal agents and exploring appropriate treatment approaches in in vivo modeling organisms are vital research projects for controlling mycoses. Caenorhabditis elegans has been proven to be a valuable tool in studies of most clinically relevant dimorphic fungi, helping to identify a number of virulence factors and immune-regulators and screen effective antifungal agents without cytotoxic effects. However, little has been achieved and reported with regard to pathogenic filamentous fungi (molds) in the nematode model. In this review, we have summarized the enormous breakthrough of applying a C. elegans infection model for dimorphic fungi studies and the very few reports for filamentous fungi. We have also identified and discussed the challenges in C. elegans-mold modeling applications as well as the possible approaches to conquer these challenges from our practical knowledge in C. elegans-Aspergillus fumigatus model.

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In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Cited by 33 publications

References 43 publications

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Caenorhabditis elegans as an Infection Model for Pathogenic Mold and Dimorphic Fungi: Applications and Challenges

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