Limnothrix redekei (Van Goor) Meffert (Cyanobacteria) Strains from Lake Kastoria, Greece Form a Separate Phylogenetic Group

Gkelis, Spyros; Rajaniemi, Pirjo; Vardaka, Elisabeth; Moustaka‐Gouni, Maria; Lanaras, T.; Sivonen, Kaarina

doi:10.1007/s00248-003-2030-7

Cited by 122 publications

(84 citation statements)

References 25 publications

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“…Diversity of Pseudanabaena populations SG Acinas et al strains isolated from Lake Kastoria (Greece), has also been observed (Gkelis et al, 2005;Willame et al, 2006). On the basis of partial sequencing of the 16-and 23S rRNA genes, all Pseudanabaena strains are isolated in this study cluster with the Pseudanabaena/Limnothrix group.…”

Section: Discussionsupporting

confidence: 60%

“…This cluster is commonly referred to as the Pseudanabaena/Limnothrix group (Gkelis et al, 2005;Willame et al, 2006). This group also comprised several environmental sequences as well as other isolates including the type strain Pseudanabaena PCC7408, strains belonging to L. redekei (Van Goor, Meffert) (Limnothrix sp.…”

Section: Discussionmentioning

confidence: 99%

“…Members of Pseudanabaena have been reported from a variety of different environments including freshwater lakes (Gkelis et al, 2005;Zwart et al, 2005;Kim et al, 2006;Willame et al, 2006), brackish environments (Stal et al, 2003), hot springs (Castenholz et al, 2001) as well as from epilithic cyanobacterial communities of beach rock (Heron Island, Great Barrier Reef) (Diez et al, 2007). Our study revealed several lineages of Pseudanabaena from the BS and from the AV, on the basis of a multilocus sequence typing approach using five loci of 28 isolates.…”

Section: Distribution and Abundance Of Pseudanabaenamentioning

confidence: 99%

“…However, often it is not recognized that smaller cyanobacteria exceed the larger species in terms of biomass and activity and therefore play a major role in the ecosystem dynamics. Several reports mentioned the occurrence of the tiny filamentous Pseudanabaena in cyanobacterial blooms in brackish and freshwater ecosystems (Vasconcelos and Pereira, 2001;Stal et al, 2003;Gkelis et al, 2005;Zwart et al, 2005;Kim et al, 2006;Willame et al, 2006). Nevertheless, Pseudanabaena spp.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria)

et al. 2008

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Pseudanabaena species are poorly known filamentous bloom-forming cyanobacteria closely related to Limnothrix. We isolated 28 Pseudanabaena strains from the Baltic Sea (BS) and the Albufera de Valencia (AV; Spain). By combining phenotypic and genotypic approaches, the phylogeny, diversity and evolutionary diversification of these isolates were explored. Analysis of the in vivo absorption spectra of the Pseudanabaena strains revealed two coexisting pigmentation phenotypes: (i) phycocyanin-rich (PC-rich) strains and (ii) strains containing both PC and phycoerythrin (PE). Strains of the latter phenotype were all capable of complementary chromatic adaptation (CCA). About 65 kb of the Pseudanabaena genomes were sequenced through a multilocus sequencing approach including the sequencing of the16 and 23S rRNA genes, the ribosomal intergenic spacer (IGS), internal transcribed spacer 1 (ITS-1), the cpcBA operon encoding PC and the IGS between cpcA and cpcB. In addition, the presence of nifH, one of the structural genes of nitrogenase, was investigated. Sequence analysis of ITS and cpcBA-IGS allowed the differentiation between Pseudanabaena isolates exhibiting high levels of microdiversity. This multilocus sequencing approach revealed specific clusters for the BS, the AV and a mixed cluster with strains from both ecosystems. The latter comprised exclusively CCA phenotypes. The phylogenies of the 16 and 23S rRNA genes are consistent, but analysis of other loci indicated the loss of substructure, suggesting that the recombination between these loci has occurred. Our preliminary results on population genetic analyses of the PC genes suggest an evolutionary diversification of Pseudanabaena through purifying selection.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Distribution and Abundance Of Pseudanabaenamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria)

et al. 2008

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“…45 More recently, Gkelis et al presented evidence that a Limnothrix strain was previously misidentified as a Planktothrix strain. 46 DNA-analyses should therefore be used as an important identification factor for culture collections, similarly to what has recently been done 47 on a small scale with a green algae collection from Germany.…”

Section: 10 Synechocystis Sp Pcc 6803mentioning

confidence: 99%

Discrimination Between Synechocystis Members (Cyanobacteria) Based on Heterogeneity of Their 16S rRNA and ITS Regions

Juteršek¹,

Klemenčič²,

Dolinar³

2017

ACSi

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Cyanobacteria are an important group of microorganisms displaying a range of morphologies that enable phenotypic differentiation between the major lineages of cyanobacteria, often to the genus level, but rarely to species or strain level. We focused on the unicellular genus Synechocystis that includes the model cyanobacterial strain PCC 6803. For 11 Synechocystis members obtained from cell culture collections, we sequenced the variable part of the 16S rRNA-encoding region and the 16S -23S internally transcribed spacer (ITS), both standardly used in taxonomy. In combination with microscopic examination we observed that 2 out of 11 strains from cell culture collections were clearly different from typical Synechocystis members. For the rest of the samples, we demonstrated that both sequenced genomic regions are useful for discrimination between investigated species and that the ITS region alone allows for a reliable differentiation between Synechocystis strains.

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