Limiting sensitivity of the method of precipitation in agar

Khramkova, N I; Абелев, Г. И.

doi:10.1007/bf00785317

Cited by 9 publications

(8 citation statements)

References 5 publications

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“…For most of the tests performed in the 2 Russian laboratories, a modified technique with double dilution of both antigen and antisera was used to enhance sensitivity. 10 Details for the preparation of antigens and antisera, and for the immunodiffusion method, are given in previous publications.% 18, 19…”

Section: Methodsmentioning

confidence: 99%

A collaborative study for the evaluation of a serologic test for primary liver cancer

O’Conor

Tatarinov

Абелев

et al. 1970

Cancer

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220

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Sera from 813 patients were examined independently by 3 test centers for the presence of alpha‐fetoprotein (AFP). The sera were collected at 5 different African centers and 2 non‐African centers. There was disagreement in the serologic results among the 3 test laboratories in only 27, or 3.3%, of cases. A clinical and/or histologic diagnosis of primary liver cancer had been made in 231 of the cases in which there was serologic agreement, and 151, or 65.4%, of these were positive for AFP. If only those cases with histologic confirmation of liver cell cancer were included, the percentage of cases with AFP increases to 75%. Analysis of the results from each collection center has been made, and the basis for “false‐positives” and serologic disagreements is discussed. The test has proven to be highly specific for primary liver cell cancer and may be used with advantage in differential diagnosis of this disease and in epidemiologic studies.

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Section: Methodsmentioning

confidence: 99%

A collaborative study for the evaluation of a serologic test for primary liver cancer

O’Conor

Tatarinov

Абелев

et al. 1970

Cancer

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220

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“…The sensitivity of determinations is known to increase directly with dilution of the test-system (Khramkova & Abelev 1961), and the faintest but still visible precipitation line assures detection of AFP Figure 1. The reagents are taken in equivalent proportions and diluted in parallel so that the precipitation line produced by them, though still clearcut, becomes progressively weaker.…”

Section: Radioimmunodiffusion Methods (Ri)mentioning

confidence: 99%

α‐Fetoprotein as a Marker of Embryo‐Specific Differentiations in Normal and Tumor Tissues

Абелев

1974

Immunological Reviews

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“…The method most commonly employed is the gel precipitation test with double-diffusion antigen antibody (57). This method with a standard test system (38) detects an antigen at the range of 1-3 jug/ml with an absolute specificity.…”

Section: Methods O F Tsg Determinationmentioning

confidence: 99%

Trophoblast-Specific Beta<sub>1</sub>-Glycoprotein as a Marker for Pregnancy and Malignancies

Tatarinov¹

1978

Gynecol Obstet Invest

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Limiting sensitivity of the method of precipitation in agar

Cited by 9 publications

References 5 publications

A collaborative study for the evaluation of a serologic test for primary liver cancer

A collaborative study for the evaluation of a serologic test for primary liver cancer

α‐Fetoprotein as a Marker of Embryo‐Specific Differentiations in Normal and Tumor Tissues

Trophoblast-Specific Beta<sub>1</sub>-Glycoprotein as a Marker for Pregnancy and Malignancies

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