Limiting Dilution Analysis of Age- and Gender-Related Differences in Autoantibody Production against Bromelain-Modified RBC

Errington, S L; Cox, K.O.

doi:10.1159/000233986

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…In this study, we also addressed the ontogeny of anti-PtC lymphocytes using the direct enumeration of liposome-binding cells. Our observation of the appearance of PtC-binding cells shows a good correlation with studies of anti-BrMRBC PFC ontogeny (33,37). The increase in abundance of PtC-specific, Ly-l' cells during the first 3 wk of life does not result from an increase in the total Ly-l' B cell population .…”

Section: Discussionsupporting

confidence: 83%

Normal mouse peritoneum contains a large population of Ly-1+ (CD5) B cells that recognize phosphatidyl choline. Relationship to cells that secrete hemolytic antibody specific for autologous erythrocytes.

Mercolino

Arnold

Hawkins

et al. 1988

The Journal of Experimental Medicine

204

123

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The most remarkable feature of the immune response is its ability to generate cells and antibodies specifically reactive with an enormous variety of foreign antigens while normally avoiding the production of autoreactive elements. An accumulating body of data demonstrates that a distinct lineage of B cells (1), the Ly-1 + B cell subset, does not display this feature, but rather is associated with the production ofautoreactive antibodies (2). We are unaware of autoimmune pathology directly demonstrated to be the result of an Ly-1 + B cell-Ig product; however, Ly-1+ B cell frequency is elevated in some autoimmune strains of mice (3). In addition to their association with autoreactive antibody, Ly-1 + B cells have been shown to have unique growth potential (1, 4); to produce most of the serum IgM found in normal, unimmunized animals (4); and the Ig repertoire of the subset may be restricted (4, 5). These findings imply that this subset has a role in normal physiology, and perhaps autoimmune pathology. A B cell subset has been identified in humans that bears the homologous marker Leu-1/T1 (CD5) (6). A similar functional role for CD5+ B cells in man has been proposed (7); human CD5+ B cells have been shown to produce autoreactive antibody (8, 9) and to be present at elevated frequency in rheumatoid arthritis (10).Normal mice produce antibodies that are reactive with protease-treated autologous erythrocytes (BrMRBC) 1 (11) ; cells producing such antibodies are present in unimmunized mice at very high frequencies, as first demonstrated by Cunningham (12) . We and others (13)(14)(15) have shown that at least some anti-BrMRBC plaqueforming cells (PFC) recognize the polar headgroup of the common membrane phospholipid, phosphatidyl choline (PtC). Most splenic anti-BrMRBC PFC have been

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Section: Discussionsupporting

confidence: 83%

Normal mouse peritoneum contains a large population of Ly-1+ (CD5) B cells that recognize phosphatidyl choline. Relationship to cells that secrete hemolytic antibody specific for autologous erythrocytes.

Mercolino

Arnold

Hawkins

et al. 1988

The Journal of Experimental Medicine

204

123

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“…The results presented here show that it enhances normal Ly-1 B cell antibody responses including secretion of autoantibodies. Others have shown that a proportion of peritoneal B cells, similar to that measured here, spontaneously secrete autoantibody [39], that this secretion is enhanced by SN from EL4 thymomas [27] and that most peritoneal B cell autoantibody secretion to BrMRBC and some other autoantigens is due to Ly-1 B cells [20,411. Here these observations are brought together and, more importantly, identify IL 5 as one of the agents responsible for enhancing Ly-1 B cell autoantibody secretion in vitro.…”

supporting

confidence: 80%

“…PFC responses to TNP-SRBC were performed in agarose using TNP-SRBC prepared as described by others [38] and guinea pig C (Gibco). Anti-bromelain-treated mouse red blood cell (BrMRBC) PFC were assayed in situ according to the method of others [39] using MRBC which had been treated for 45 min at 37°C with 20 mg/ml of bromelain (Fluka, Buchs, Switzerland) using equal volumes of packed cells and bromelain/PBS solution.…”

Section: Antibody Secretionmentioning

confidence: 99%

Interleukin 5 regulation of peritoneal Ly‐1 B lymphocyte proliferation, differentiation and autoantibody secretion

Wetzel¹

1989

Eur J Immunol

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The in vitro effects of recombinant interleukin (IL) 5 on proliferation and maturation of mouse Ly-1 B cells were studied. Most freshly isolated peritoneal Ly-1 B cells expressed high levels of IL5 receptor (R). Limiting dilution analyses showed that mitogens could reveal IL5 responsiveness in more than half of low density peritoneal Ly-1 B cells. IL 5 was able not only to increase the proportion of these Ly-1 B cells induced to proliferate, but it also shifted the clone size distribution of proliferating cells towards larger clone sizes. Splenic Ly-1 B cells also proliferated in response to mitogens plus IL5. Spontaneous and polyclonal activator-induced plaque-forming cell responses of Ly-1 B cells were increased by IL5. Furthermore, IL5 increased the frequency of peritoneal Ly-1 B cells induced to secrete certain autoantibodies. IL5 was certainly the agent responsible since its effects on both proliferation and differentiation were inhibited by either anti-IL5R monoclonal antibodies or by anti-IL5 monoclonal antibodies. Hence, Ly-1 B cells, IL5 and the IL5R appear to constitute a system of cellular proliferation, differentiation and some autoantibody production. Strategies specifically targeting the interleukin and receptor elements of this system might afford external control of these cellular responses.

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“…Uncultured spleen cells and cells harvested from 96-well plates were assayed according to the method of Cunningham and Szenberg [15], The assay procedure for cells cultured in Terasaki trays has been described elsewhere [ 18].…”

Section: Assay For Autoantibody Productionmentioning

confidence: 99%

Effect of Whole-Body Irradiation on Precursor Frequency of Autoantibody-Secreting Cells and Plaque-Forming Cells Specific for Bromelain-Modified Erythrocytes

Errington¹,

Cox²

1987

Int Arch Allergy Immunol

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Murine spleen cells and peritoneal cells were assayed for autoantibody production against bromelain-modified mouse erythrocytes (mouse brom-RBC) after whole-body irradiation at a dose of 5 Gy. Spleens were taken directly from mice 3 days after irradiation, or cultured for 3 days 24 h after irradiation, and assayed. Cells taken directly from irradiated mice had a higher number of plaque-forming cells (PFC) against mouse brom-RBC, compared with unirradiated mice, when the data were expressed as PFC/10⁶ viable cells. This apparent increase can be accounted for by the reduced number of cells in the spleens from irradiated mice. In both spleen cells and peritoneal cells cultured in high cell densities, no difference was observed in the number of PFC in cells from irradiated or unirradiated mice. The detected precursor frequency of autoimmune B cells in both cell populations cultured in limiting dilution was found to be lower in cells from irradiated mice. In contrast to other published data, it was concluded from these investigations that whole-body irradiation, at a dose of 5 Gy, does not augment autoantibody production against mouse brom-RBC. It is proposed that the reason for this is the presence of a regulatory cell that is relatively radioresistant and, hence, not removed after whole-body irradiation at a dose of 5 Gy.

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Limiting Dilution Analysis of Age- and Gender-Related Differences in Autoantibody Production against Bromelain-Modified RBC

Cited by 10 publications

References 10 publications

Normal mouse peritoneum contains a large population of Ly-1+ (CD5) B cells that recognize phosphatidyl choline. Relationship to cells that secrete hemolytic antibody specific for autologous erythrocytes.

Normal mouse peritoneum contains a large population of Ly-1+ (CD5) B cells that recognize phosphatidyl choline. Relationship to cells that secrete hemolytic antibody specific for autologous erythrocytes.

Interleukin 5 regulation of peritoneal Ly‐1 B lymphocyte proliferation, differentiation and autoantibody secretion

Effect of Whole-Body Irradiation on Precursor Frequency of Autoantibody-Secreting Cells and Plaque-Forming Cells Specific for Bromelain-Modified Erythrocytes

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