Interleukin 5 regulation of peritoneal Ly‐1 B lymphocyte proliferation, differentiation and autoantibody secretion

Wetzel, Gayle Delmonte

doi:10.1002/eji.1830190926

Cited by 66 publications

(32 citation statements)

References 39 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, these findings suggest that the B-1a B cells in SMGs and NPs play key roles in the induction of TI Ag-specific mucosal IgA Ab responses in the oral-nasopharyngeal mucosa in addition to the gastrointestinal tract. The B-1 B cell subset has been shown to constitutively express IL-5R␣ (17,24) and to exhibit modest levels of proliferation and differentiation when treated with IL-5 (25)(26)(27). These studies clearly indicate that IL-5 plays a central role in B-1 B cell activation.…”

Section: Discussionmentioning

confidence: 78%

Nasal Cholera Toxin Elicits IL-5 and IL-5 Receptor α-Chain Expressing B-1a B Cells for Innate Mucosal IgA Antibody Responses

Kataoka

Fujihashi

Sekine

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

In this study, we examine whether native cholera toxin (nCT) as a mucosal adjuvant can support trinitrophenyl (TNP)-LPS-specific mucosal immune responses. C57BL/6 mice were given nasal TNP-LPS in the presence or absence of nCT. Five days later, significantly higher levels of TNP-specific mucosal IgA Ab responses were induced in the nasal washes, saliva, and plasma of mice given nCT plus TNP-LPS than in those given TNP-LPS alone. High numbers of TNP-specific IgA Ab-forming cells were also detected in mucosal tissues such as the nasal passages (NPs), the submandibular glands (SMGs), and nasopharyngeal-associated lymphoreticular tissue of mice given nCT. Flow cytometric analysis showed that higher numbers of surface IgA+, CD5+ B cells (B-1a B cells) in SMGs and NPs of mice given nasal TNP-LPS plus nCT than in those given TNP-LPS alone. Furthermore, increased levels of IL-5R α-chain were expressed by B-1a B cells in SMGs and NPs of mice given nasal TNP-LPS plus nCT. Thus, CD4+ T cells from these mucosal effector lymphoid tissues produce high levels of IL-5 at both protein and mRNA levels. When mice were treated with anti-IL-5 mAb, significant reductions in TNP-specific mucosal IgA Ab responses were noted in external secretions. These findings show that nasal nCT as an adjuvant enhances mucosal immune responses to a T cell-independent Ag due to the cross-talk between IL-5Rα+ B-1a B cells and IL-5-producing CD4+ T cells in the mucosal effector lymphoid tissues.

show abstract

Section: Discussionmentioning

confidence: 78%

Nasal Cholera Toxin Elicits IL-5 and IL-5 Receptor α-Chain Expressing B-1a B Cells for Innate Mucosal IgA Antibody Responses

Kataoka

Fujihashi

Sekine

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Importantly, IL-5 also induces proliferation and differentiation of B cells, particularly B-1 cells, which constitutively express the IL-5 receptor (31,(43)(44)(45). Accordingly, IL-5 transgenic mice have (in addition to eosinophilia) increased numbers of B-1 cells in the peritoneal cavity and elevated levels of serum IgM and IgA (46), while IL-5Rα -/-mice do not show IL-5-induced enhancement of B cell responses to TI type 2 (TI-2) antigens (47).…”

Section: Discussionmentioning

confidence: 99%

“…Although many stimuli, including antigen, LPS and cytokines, have been demonstrated to activate B-1 cells, the exact physiological triggers during an orchestrated immune response are not clear. IL-5 specifically plays a role in stimulating IgM secretion by B-1 cells (43,49). Moreover, IL-10 seems to be involved in B-1 cell activation as well (44,50), although partially in an autocrine manner (51), whereas IL-12 has been shown to inhibit B-1 cell proliferation (52).…”

Section: Discussionmentioning

confidence: 99%

IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis

Binder

Hartvigsen²,

Chang³

et al. 2004

J. Clin. Invest.

254

241

View full text Add to dashboard Cite

“…Ly-l+ [12]. can also act as a maturation factor on a small but significant population of small resting IL5R+ B cells.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the interleukin 5‐reactive splenic B cell population

Rolink

Thalmann²,

Kikuchi³

et al. 1990

Eur J Immunol

View full text Add to dashboard Cite

The characteristics of the interleukin (IL) 5-reactive splenic B cell population of C57BL/6 nu/nu mice, with respect to IL 5/IL2 reactivity, cell surface phenotype, VH gene family usage, autoreactivity and the structure of the IL5 receptor (IL5R), were analyzed. It was found that 2%-4% of splenic B cells express relatively high levels of IL 5R as determined by the binding of the anti-IL 5R monoclonal antibody R52.120. Over 90% of the splenic B cells that mature to IgM secretion upon activation with IL5 are comprised in this small subpopulation of B cells. Moreover, the vast majority of splenic B cells that mature to IgM-secreting cells when activated by IL2 also reside in this IL5R+B cell population. The cell surface phenotype of the IL5R+ splenic B cells is IgM+, B220+, Ly-1- and IL2R p55-. Upon activation with IL5 this cell surface phenotype changes, in that a vast majority of the B cells then express the p55 chain of the IL2R, whereas the level of IL5R decreases. VH gene family usage in the IL5-activated splenic B cells was analyzed by in situ hybridization. VH gene family usage was found to be random and not different from the VH genes expressed in LPS-activated B cells. Hybridoma collections from IL5-activated splenic B cells and LPS-activated B cells were screened and compared for the production of autoantibodies and antibodies directed against the haptens (4-hydroxy-3-iodo-5-nitrophenyl)acetyl (NIP) and 2,4,6-trinitrophenyl (TNP). In both collections high, but not significantly different frequencies of autoantibody-(32% IL5, 31.4% LPS) and of anti-hapten antibody (27.8% IL5, 18.6% LPS)-producing hybridomas were found. The structure of the IL5R on IL5-activated B cells was analyzed by 125I-labeled IL5 binding and cross-linking. About 100 high-affinity (10(-11) M) and 1000 low-affinity (10(-9) M) IL5-binding sites are present on IL5-activated splenic B cells, and both high- and low-affinity IL5R are similar to those expressed on the IL5-dependent B13 cell line. Cross-linking of 125I-labeled IL5 to the receptors on IL5-activated B cells revealed one major IL5-binding protein of 45-50 kDa molecular mass and another minor binding protein of 130-140 kDa. The same IL5-binding proteins are present on the IL5-dependent B13 cell line.(ABSTRACT TRUNCATED AT 400 WORDS)

show abstract

Interleukin 5 regulation of peritoneal Ly‐1 B lymphocyte proliferation, differentiation and autoantibody secretion

Cited by 66 publications

References 39 publications

Nasal Cholera Toxin Elicits IL-5 and IL-5 Receptor α-Chain Expressing B-1a B Cells for Innate Mucosal IgA Antibody Responses

Nasal Cholera Toxin Elicits IL-5 and IL-5 Receptor α-Chain Expressing B-1a B Cells for Innate Mucosal IgA Antibody Responses

IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis

Characterization of the interleukin 5‐reactive splenic B cell population

Contact Info

Product

Resources

About