Limiting amino acids in raw and processed amaranth grain protein from biological tests

Bressani, Ricardo; Elías, L. G.; Garcia-Soto, A.

doi:10.1007/bf01091933

Cited by 24 publications

(15 citation statements)

References 12 publications

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“…When compared with soybean, a significantly higher concentration of glutamine, glycine, and methionine was found in amaranth, while tyrosine and cysteine and the essential amino acids (e.g. When considering the chemical score, several authors indicate leucine as the limiting amino acid in amaranth (Becker et al, 1981;Saunders and Becker, 1984;Pederson et al, 1987;Abreu et al, 1994;Escudero et al, 2004), whereas when considering the protein efficiency ratio (PER), threonine was recognized to be the limiting amino acid (Bressani et al, 1989). Both amaranth and soybean had comparable or higher amounts of amino acids than whole egg protein.…”

Section: Amino Acidsmentioning

confidence: 99%

Pseudocereals

Schoenlechner¹,

Siebenhandl²,

Berghofer³

2008

Gluten-Free Cereal Products and Beverages

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Section: Amino Acidsmentioning

confidence: 99%

Pseudocereals

Schoenlechner¹,

Siebenhandl²,

Berghofer³

2008

Gluten-Free Cereal Products and Beverages

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“…A suitable content of lysine and tryptophan together with low content of leucine makes it a high-quality supplement for e.g. maize, which is rich in leucine but poor in lysine and tryptophan (Correa et al, 1986;Imeri et al, 1987a;Bressani et al, 1989). Amaranth oil is rich in unsaturated fa�y acids, especially linoleic acid and oleic acid (Lorenz and Hwang, 1985;Yanez et al, 1994); the content of squalene (5 to 6%) is also important.…”

mentioning

confidence: 99%

The use of amaranth grain in diets for broiler chickens and its effect on performance and selected biochemical indicators

Roučková¹,

Trčková²,

Herzig³

2004

Czech J. Anim. Sci.

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In the Czech Republic, meat-and-bone meals are banned ingredients in the manufacture of feeds for farm animals. Therefore alternative sources providing proteins, limiting amino acids and energy in the ration have to be found. Out of vegetable protein feeds, rape, soybean, peanut and legumes are especially considered, and to a lesser extent other oil plants and products of the oil industry, i.e. oil cakes or extracted meals. Certain limitations of use exist in all the above-mentioned feeds, e.g. in connection with the content of antinutritional substances (erucic acid and glucosinolates), moulds and their toxic products, etc. (Herzig, 2001).Among plants that meet the requirements for feeds that replace meat-and-bone meals, amaranth and its products appear to be suitable, being classified as pseudocereals. The genus Amaranthus (L) belongs to the family Amaranthaceae and includes more than 60 species. Under our conditions, three grain species Amaranthus cruentus, Amaranthus hypochondriacus and Amaranthus caudatus are of importance. The genotypes suitable for cultivation in the Czech Republic, environmental requirements and technological conditions under which the plants can be grown are already well known. Experience obtained in the cultivation of amaranth to date has shown that field yields range from 0.6 to 2.4 t/ha (Jarošová et al., 1997).Amaranth has a high nutritional value. The dry ma�er of amaranth grain contains 12.6 to 18.0% of proteins, 5 to 8% of fat, 60 to 65% of saccharides, and 3 to 5% of crude fibre (Cole, 1979;Bressani et al., 1993;Yanez et al., 1994). The advantage of amaranth grains compared to conventional cereals is a relatively high content of proteins and more balanced amino acid composition. Amaranth grain is ABSTRACT:The objective of our experiment was to test the possibility of using amaranth grain, either heat-treated (AO) or without treatment (AN), in vegetable diets for broilers as a substitution for meat-and-bone meals. The effect of amaranth on performance and selected biochemical parameters was investigated. The groups of chickens fed with amaranth obtained comparable results in all characteristics with the control group whose diet included a component of animal origin. We did not observe any statistical differences in live weights of monitored groups of chickens on day 41 (Kab 2 149.9 ± 274.3; ANab 2 192.2 ± 255.2; AOab 2 186.2 ± 260.8 g). Feed conversion ranged from 1.9 kg in the control group of hens to 2.2 kg in the experimental group of cocks AOa. Carcass yield was significantly higher (P < 0.05) in the control group compared to the group AN. Biochemical characteristics, i.e. the concentrations of proteins, total lipids, cholesterol and glucose in blood sera of broiler chickens were monitored. The inclusion of amaranth in the rations of experimental groups had no effect on protein concentrations compared to the control group. The hypocholesteraemic and hypolipidaemic effects of amaranth grain, as mentioned in the literature, were not confirmed in our experiment. Glucose levels w...

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“…caudatus L.) produce edible seeds and are an important food crop in several areas of Latin America and Africa [12]. Amaranth seed protein has an exceptional balance of amino acids and an average seed protein content (15% on a dry matter basis) that is notably higher than most cereal grains [13, 14]. Despite the relative minor status of the grain amaranths as an alternative crop, important genomic tools are being developed that should aid in the genetic improvement of the species.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

Maughan

Smith

Raney

2012

Journal of Biomedicine and Biotechnology

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Bacterial artificial chromosome (BAC) libraries are critical for identifying full-length genomic sequences, correlating genetic and physical maps, and comparative genomics. Here we describe the utilization of the Fluidigm access array genotyping system in conjunction with KASPar genotyping technology to identify individual BAC clones corresponding to specific single-nucleotide polymorphisms (SNPs) from an Amplicon Express seven-plate super pooled Amaranthus hypochondriacus BAC library. Ninety-six SNP loci, spanning the length of A. hypochondriacus linkage groups 1, 2, and 15, were simultaneously tested for clone identification from four BAC super pools, corresponding to 28 384-well plates, using a single Fluidigm integrated fluidic chip (IFC). Forty-six percent of the SNPs were associated with a single unambiguous identified BAC clone. PCR amplification and next-generation sequencing of individual BAC clones confirmed the IFC clone identification. Utilization of the Fluidigm Dynamic array platform allowed for the simultaneous PCR screening of 10,752 BAC pools for 96 SNP tag sites in less than three hours at a cost of ~$0.05 per reaction.

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Limiting amino acids in raw and processed amaranth grain protein from biological tests

Cited by 24 publications

References 12 publications

Pseudocereals

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The use of amaranth grain in diets for broiler chickens and its effect on performance and selected biochemical indicators

Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept

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