Limited-resource preparable chitosan magnetic particles for extracting amplification-ready nucleic acid from complex biofluids

Abstract

We present methods for limited-resource-friendly preparation of chitosan magnetic particles that are amenable to ultrasensitive downstream isothermal and conventional nucleic acid amplification tests.

Rapid and sensitive detection of Staphylococcus aureus via an all-in-one staggered strand exchange amplification platform

“…17 It is noteworthy that adoption of magnetic beads (MBs) to enrich and purify DNA from complex biological fluids significantly lowered the detection limit of LAMP or PCR. 18 Due to their large surface area, good biocompatibility, and ease of operation, the application of MBs in DNA extraction and detection has gained extensive interest in recent years.…”

Chitosan: a green adhesive for surface functionalization and fabrication of thermoplastic biomedical microdevices

“…12 Furthermore, native CS can be used as a DNA capture material for DNA purification and sample preparation. [13][14][15] Recently, magnetic particles have been surface-decorated with CS via coprecipitation or crosslinking to capture, purify, and amplify DNA from various samples. 14 Byrnes et al printed chitosan on a porous membrane to realize the purification and concentration of nucleic acids in complex samples on a lateral flow strip.…”

Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples

“…Magnetic beads (MBs) with a functionalized surface that enables nucleic acid capture have been widely used for nucleic acid extraction from biological samples, based on which a variety of formats have been developed, such as manual extraction by a magnetic rack or microfluidic chips [15,16], as well as automated robotic processing [17,18]. Compared with the traditional nucleic acid extraction approaches, such as spin column-based methods, thermal lysis, and alkaline lysis, MB-based methods combine the advantages of simple processing, short time consumption, high product purity, and easy integration for high-throughput detection, making them the preferred nucleic acid extraction methods for pathogen detection [19,20]. In the process of using the traditional MB-based method, once the nucleic acids are captured on MBs, they are isolated from samples, followed by washing with alcohols and eluting into nuclease-free water, and then application as templates for NAATs.…”