Limited immunotoxic potential of technical formulation of the herbicide atrazine (AAtrex) in mice

Fournier, Michel; Friborg, Jacques; Girard, Denis; Mansour, Saad; Krzystyniak, Krzysztof

doi:10.1016/0378-4274(92)90284-q

Cited by 44 publications

(23 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, our findings contrast with those of Founier et a/. [7] and Desjardins et a/. [8] who reported suppression in only the primary humoral response with N-nitrosodimethylamine and atrazine treatment.…”

contrasting

confidence: 99%

Comparison of humoral and cell mediated immune response modulation by chemical and bio‐pesticides

Bhatia

Kaur²

2004

International Journal of Environmental Studies

View full text Add to dashboard Cite

The awareness about the negative impact of chemical pesticides on health and, also their indispensability for the agriculture sector, has raised the need for new safer pesticides, in the form of biopesticides. In our previous studies [l-3], it was found that chemical pesticides generally suppress the immune response, but there is hardly any study in which the impact of biopesticides on the immune response has been examined. In the present study a comparison of humoral and cell mediated immune response modulation by three chemical pesticides, i.e. malathione(M), methyl parathione (MP) and dimethoate (DM) and a plantbased commercial biopesticide, Neemazal (NAF), purchased from EID Parry India Ltd Madras) was carried out in vivo in swiss albino mice. The animals, divided into four groups, each consisting of 10 mice, were given either a sublethal dose of one of the pesticides (test) or distilled water only (control) in 10 inoculations, intraperitoneally, over a period of 25-30 days. Animals were immunized with SRBC during the course of inoculations with the pesticide. The blood samples were collected on, the 4"', 7* and ll"* day post-SRBC inculation to check the development of an anti-SRBC antibody and a p mercaptoethanol resistant anti-SRBC antibody (IgG); they were then sacrificed on day 11 post-SRBC inoculation to assess the percent leukocyte migration inhibition (LMI), percent leukocyte adherence inhibition and percent nitroblue tetrazolium chloride reduction by the splenocytes by using the methodology given in the Manual by Bhatia [4]. RESULTSTables I and II showed that in chemical pesticides, i.e. M or MP or DM treated animals the anti-SRBC antibody titres were lower, whereas in the NAF-treated animals the titres were higher than in control animals. Similarly, the percentages of LMI, LAI and NBT were lower •Corresponding author.

show abstract

contrasting

confidence: 99%

Comparison of humoral and cell mediated immune response modulation by chemical and bio‐pesticides

Bhatia

Kaur²

2004

International Journal of Environmental Studies

View full text Add to dashboard Cite

show abstract

“…As noted in the introduction, atrazine has been repeatedly demonstrated to be immunotoxic both in vitro and in vivo (Fournier et al 1992;Hooghe et al 2000). This report has further characterized the effects of a direct 24 h atrazine exposure on human NK cell lytic function by demonstrating that atrazine significantly inhibits human PBL lysis of NK sensitive targets cells in a concentration dependent manner (Figure 1), and sought to identify a subcellular effect of atrazine exposure which would explain this reduction in NK-specific lytic function.…”

Section: Discussionmentioning

confidence: 79%

“…Mencoboni et al 1992 found that acute intraperitoneal exposure to atrazine reduced hematopoietic progenitors for a short period. A transient reduction in IgM production and T cell reactivity has also been reported after an acute oral exposure (Fournier et al, 1992). Subchronic (2 week) exposure to atrazine decreased resistance to tumor challenge (Karrow et al, 2005).…”

mentioning

confidence: 76%

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

Rowe

Brundage

Barnett

2007

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

The herbicide atrazine is a known immunotoxicant and an inhibitor of human Natural Killer (NK) cell lytic function. The precise changes in NK cell lytic function following atrazine exposure have not been fully elucidated. The current study identifies the point at which atrazine exerts its affect on the stepwise process of human NK cell mediated lyses of the K562 target cell line. Using intracellular staining of human peripheral blood lymphocytes, it was determined that a 24 h in vitro exposure to atrazine did not decrease the level of NK cell lytic proteins granzyme A, granzyme B or perforin. Thus, it was hypothesized that atrazine exposure was inhibiting the ability of the NK cells to bind to the target cell and subsequently inhibit the release of lytic protein from the NK cell. To test this hypothesis, flow cytometry and fluorescent microscopy were employed to analyze NK cell-target cell co-cultures following atrazine exposure. These assays demonstrated no significant decrease in the level of target cell binding. However, the levels of NK intracellular lytic protein retained and the amount of lytic protein released were assessed following a 4 h incubation with K562 target cells. The relative level of intracellular lytic protein was 25-50% higher, and the amount of lytic protein released was 55-65% less in atrazine treated cells than vehicle treated cells following incubation with the target cells. These results indicate that ATR exposure inhibits the ability of NK cells to lyse target cells by blocking lytic granule release without affecting the ability of the NK cell to form stable conjugates with target cells.

show abstract

“…Exposure to ATR for 14 days can induce a significant decrease in the number of hematopoietic progenitors in murine bone marrow (16), cause a transient suppression of IgM production and T cell proliferation (17), decrease the CD4+ lymphocytes and MHC-II+ cells (18), and increase the mixed lymphocyte reaction (MLR) and cytotoxic T lymphocyte (CTL) response (19). Rajkovic et al reported that ATR increased the number of degranulated mast cells of rats (20).…”

mentioning

confidence: 99%

Sub-acute exposure to the herbicide atrazine suppresses cell immune functions in adolescent mice

et al. 2013

View full text Add to dashboard Cite

Limited immunotoxic potential of technical formulation of the herbicide atrazine (AAtrex) in mice

Cited by 44 publications

References 17 publications

Comparison of humoral and cell mediated immune response modulation by chemical and bio‐pesticides

Comparison of humoral and cell mediated immune response modulation by chemical and bio‐pesticides

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

Sub-acute exposure to the herbicide atrazine suppresses cell immune functions in adolescent mice

Contact Info

Product

Resources

About