Limbal Stem-Cell Transplantation

Ek, Akpek; Cs, Foster

doi:10.1097/00004397-199903910-00009

Cited by 12 publications

(13 citation statements)

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“…Stem cells are responsible for tissue replacement and regeneration (Akped and Foster, 1999). They are essential to maintain the integrity of corneal surface, by promoting its renewal in healthy states and reepithelialization in wound healing processes (Swift et al, 1996;Haamann et al, 1998;Dua and Azuara-Blanco, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Limbal deficiency or loss of stem cells is characterized by a reduction of proliferative capacity, resulting in abnormal corneal surface (Akped and Foster, 1999). In such occasions, there is growth of the conjunctival epithelium onto the corneal surface (conjunctivalization), as well as chronic inflammation, superficial vascularization, calcification, ulceration, melting and perforation of the cornea (Swift et al, 1996;Tseng and Tsubota, 1997;Dua and AzuaraBlanco, 1999;Dua and Azuara-Blanco, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Sclerocorneal limbal stem cell autograft transplantation in dogs

Brunelli

Vicente

Chahud

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The effects of sclerocorneal limbal stem cell autograft transplantation in dogs with corneal wounds were studied. Eighteen dogs were divided in two groups (GI and GII). The animals of GI (n=12) underwent limbal transplantation 30 days after the destruction of limbal stem cells. The dogs of GII (n=6) only underwent destruction of stem cells (control group). Light microscopy examination of the right eye was performed on days 3, 7, 14, 30, 60, and 120 after limbal transplantation (GI), and on days 33, 37, 44, 60, 90, and 150 after limbal destruction (GII). Results showed a complete destruction of limbal stem cells with loss of corneal transparency. Limbal transplantation prevented conjunctivalization in grafted area. Corneal vascularization and a 360º corneal conjunctivalization were noted in the control dogs (GII). Corneal transparency was restored from day 60th after surgery. Histological examination did not distinguish the transition between the graft and the normal corneal epithelium at anytime. Goblet cells were found in control animals (GII) on 33, 37, 60, and 150 days, whereas a single grafted dog (GI) presented a few goblet cells on day 60th post-transplantation. Limbal autograft transplantation was effective in restoring corneal clarity with no development of ocular complications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sclerocorneal limbal stem cell autograft transplantation in dogs

Brunelli

Vicente

Chahud

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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“…It differs morphologically from the cornea in that it possesses Langerhans cells and melonocytes, and from the conjunctiva in that it lacks a layer of goblet cells ). The limbus is considered a specialized conjunctiva, which is composed of over 10 layers of cells (Wagoner, 1997;Akped and Foster, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Stem cells are responsible for tissue replacement and regeneration (Kruse et al, 1990;Akped and Foster, 1999). They are essential in maintaining the integrity of corneal surface, by promoting its renewal in healthy states and reepithelialization in wound healing processes (Huang and Tseng, 1991;Swift et al, 1996;Haamann et al, 1998;Dua and Azuara-Blanco, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Limbal deficiency or loss of stem cells are characterized by a reduction of proliferative capacity, resulting in abnormal corneal surface (Akped and Foster, 1999). In such occasions, there is growth of the conjunctival epithelium onto the corneal surface (conjunctivalization), as well as chronic inflammation, superficial vascularization, calcification, ulceration, melting and perforation of the cornea (Swift et al, 1996;Dua and Azuara-Blanco, 1999;Dua and AzuaraBlanco, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Excision of sclerocorneal limbus in dogs and resulting clinical events: study of an experimental model

Brunelli

Vicenti

Oriá

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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The consequences of complete removal of the sclerocorneal limbus epithelium were evaluated, as well as the model for such, in which n-heptanol and superficial keratectomy were used. The association of nheptanol and keratectomy allowed a complete excision of stem cells. Moreover, the destruction of these cells led to significant tectonic alterations that could be employed as a feasible model for clinical experimentation of corneal transplantation.Keywords: dog, stem cell, cornea RESUMO As conseqüências da completa remoção do epitélio do limbo esclerocorneano foram avaliadas

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Die Limbustransplantation zur Rekonstruktion der Augenoberfläche

Kruse

Reinhard

2001

Der Ophthalmologe

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Proliferation of the corneal epithelium originates in undifferentiated, long-lived stem cells that are located in the basal limbal epithelium. Stem cells are important for corneal epithelial regeneration and wound healing. Depletion of stem cells due to accidents as well as malfunctions of stem cells due to inborn or inflammatory diseases result in limbal stem cell deficiency. Limbal deficiency is characterized by conjunctivalization of the cornea with vascularization and opacification. Partial limbal deficiency can be treated by removing ingrown conjunctival epithelium thus allowing normal limbal epithelium to repopulate the cornea. Unilateral limbus-derived stem cell disease requires either limbal autograft transplantation from the healthy partner eye or kerato-limbal allograft transplantation. Several modifications of the latter technique have been performed including large kerato-limbal lamellar grafts and central penetrating kerato-limbal allografts. All homologous procedures render a very high risk of immunological reactions that require long term systemic immunosuppression. The use of amniotic membrane, better pharmacological drugs for immunosuppression and improvements in the HLA-matching of limbal allografts as well as ex vivo expansion of corneal stem cells should allow for better reconstruction of the ocular surface in limbal deficiency.

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Limbal Stem-Cell Transplantation

Cited by 12 publications

References 0 publications

Sclerocorneal limbal stem cell autograft transplantation in dogs

Sclerocorneal limbal stem cell autograft transplantation in dogs

Excision of sclerocorneal limbus in dogs and resulting clinical events: study of an experimental model

Die Limbustransplantation zur Rekonstruktion der Augenoberfläche

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