Limb Allografts in Rats Immunosuppressed with Cyclosporine

Hotokebuchi, Takao; Arai, Ken; Takagishi, Kenji; Arita, Chikafumi; Sugioka, Yôichi; Kaibara, N.

doi:10.1097/00006534-198906000-00017

Cited by 55 publications

(10 citation statements)

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“…The intramedullary fixation of the femur was achieved with an 1 B-gauge needle, and the muscles were reapproximated, but the nerves were left unsutured. These procedures were in accordance with those of Hotokebuchi et al [7] except for preserv ing the femoral vessels. Ischemia w'as induced by applying a microvascular clamp.…”

Section: Rat Hindlimb Preparationmentioning

confidence: 94%

Implication of Superoxide Radicals on Ischemia-Reperfusion-lnduced Skeletal Muscle Injury in Rats

Kawasaki

Sugiyama

et al. 1993

Eur Surg Res

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This study was designed to clarify the mechanism of ischemia-reperfusion injury to skeletal muscle using long-acting polyoxyethylene-modified superoxide dismutase (SOD-POE) in rats. The gastrocnemius muscles of male Lews rats were investigated. Tissue levels of ATP decreased to 20% of nonischemic values after 3 h ischemia and returned to 94% after 1 h reperfusion. In contrast, they decreased to 3.5% after 4 h ischemia and remained at 20% after 1 h reperfusion. Although SOD-POE did not affect the decrease of ATP during ischemia, it improved significantly the recovery of ATP: 28%. Tissue levels of lipid peroxides (LPO) after 3 h ischemia and 1 h reperfusion did not change significantly compared with the nonischemic levels (0.71 ± 0.32 nmol/mg protein, mean ± SD). They showed no increase after 4 h ischemia, but increased explosively after 1 h reperfusion (2.15 ± 0.73 nmol/mg protein). SOD-POE did not affect LPO levels during ischemia but prevented the increase of LPO significantly after reperfusion (0.98 ± 0.25 nmol/mg protein). Xanthine oxidase activity did not increase after 3 h ischemia (22.3 ± 7.0 mU/g) compared with the nonischemic values (17.6 ± 10.0 mU/g). In contrast, it increased 2.5-fold after 4h ischemia (50.1 ± 13.7 mU/g) and remained at a significantly high level after 1 h reperfusion. SOD-POE did not affect xanthine oxidase activity during ischemia and reperfusion. These results suggest that lipid peroxidation by superoxide radicals produced by xanthine oxidase is a contributory factor to ischemia-reperfusion injury to skeletal muscle, and the clinical application of SOD-POE might be expected.

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Section: Rat Hindlimb Preparationmentioning

confidence: 94%

Implication of Superoxide Radicals on Ischemia-Reperfusion-lnduced Skeletal Muscle Injury in Rats

Kawasaki

Sugiyama

et al. 1993

Eur Surg Res

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“…The amputated mus cles were then roughly anatomically apposed using 4-0 nylon sutures, but the sciatic nerve was not repaired. The above-described method was performed accord ing to Hotokcbuchi et al [7] with the exception that the femoral vessels were preserved. For dmg infusions, the left femoral vein was exposed, and a 24-gauge nylon tube inserted into it and fixed with a 5-0 silk suture.…”

Section: Methodsmentioning

confidence: 99%

Effect of Dibutyryl Cyclic Adenosine Monophosphate on Skeletal Muscle Reperf usion Injury in the Rat

Ohshima¹,

Yabe²,

Ishiguro³

et al. 1997

Eur Surg Res

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To clarify the action of dibutyryl cyclic adenosine monophosphate (DBcAMP) on reperfused ischemic muscle, experiments were conducted using the hindlimbs of 18 male Lewis rats. At the midportion of the thigh all tissues except for the femoral artery and vein were transected, and a route for continuous intravenous infusion was secured in a contralateral limb vein. After inducing total ischemia by clamping the femoral artery and vein with a vascular clamp for 4 h, the limb was reperfused for 1 h. Blood flow was then compared using the hydrogen gas clearance method in a group in which DBcAMP 10 mg was continuously infused from a vein in the contralateral hindlimb from 1 h prior to the induction of ischemia to 1 h after the completion of ischemia (DBcAMP group), a group in which saline was infused in the same manner (control group), and a group which was subjected to biopsy alone (biopsy group). The percent change in blood flow was significantly higher in the DBcAMP group than in the control group at 15 and 30 min after the release of the clamp. Adenosine triphosphate (ATP), phosphocreatine (PCr), and lipid peroxide (LPO) were measured in tissue samples obtained 1 h after reperfusion. Serum LPO was measured in blood samples collected at the same time. ATP values were higher in the DBcAMP group than in the control group. PCr was significantly higher in the DBcAMP group than in the control group. LPO levels in skeletal muscle tissue did not differ significantly between the DBcAMP and control groups. In contrast, serum LPO levels were significantly lower in the DBcAMP group than the control group. On morphologic analysis the control and DBcAMP groups showed normal vascular endothelial cells and absence of the ‘no-reflow’ phenomenon. These data confirm that in this reperfusion model the administration of DBcAMP enhances the viability of skeletal muscle cells. Moreover, mediated by an effect on vascular endothelial cells this agent is thought to be of help in mitigating the vascular endothelial cell injury occurring in acute ischemic injury. DBcAMP may be a useful agent in mitigating skeletal muscle ischemia-reperfusion injury.

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“…The pathologic changes in the articular cartilage of the knee joint after composite tissue transplantation were evaluated by two blinded observers as described by Hotokebuchi et al 14 .…”

Section: Histological Studymentioning

confidence: 99%

Acute Rejection of Knee Joint Articular Cartilage in a Rat Composite Tissue Allotransplantation Model

Shibuya

Imai

Lee

et al. 2014

The Journal of Bone and Joint Surgery

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Limb Allografts in Rats Immunosuppressed with Cyclosporine

Cited by 55 publications

References 0 publications

Implication of Superoxide Radicals on Ischemia-Reperfusion-lnduced Skeletal Muscle Injury in Rats

Implication of Superoxide Radicals on Ischemia-Reperfusion-lnduced Skeletal Muscle Injury in Rats

Effect of Dibutyryl Cyclic Adenosine Monophosphate on Skeletal Muscle Reperf usion Injury in the Rat

Acute Rejection of Knee Joint Articular Cartilage in a Rat Composite Tissue Allotransplantation Model

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