Acute Rejection of Knee Joint Articular Cartilage in a Rat Composite Tissue Allotransplantation Model

Shibuya, Nobuhito; Imai, Yoshimichi; Lee, Yang Sung; Kochi, Takashi; Tachi, Masahiro

doi:10.2106/jbjs.m.00859

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Allogeneic chondrocytes are more accessible, but cartilage produced by them in articular surface defects in rats was resorbed by infiltrating immune cells [ 50 , 51 ]. This acute rejection of newly formed allocartilage was not prevented even by the strong immunosuppressive agents cyclosporine A (CsA) and cladribine (2-chlorodeoxyadenosine) [ 52 ]. Allogeneic cartilage formed by chondrocytes transplanted into articular cartilage defects was infiltrated in its deep part, submerged in subchondral bone and bone marrow, while on the surface of the transplant facing the joint cavity infiltrating cells were absent [ 50 , 52 ].…”

Section: Immunogenic Properties Of Chondrocytes – Implications For Chmentioning

confidence: 99%

“…This acute rejection of newly formed allocartilage was not prevented even by the strong immunosuppressive agents cyclosporine A (CsA) and cladribine (2-chlorodeoxyadenosine) [ 52 ]. Allogeneic cartilage formed by chondrocytes transplanted into articular cartilage defects was infiltrated in its deep part, submerged in subchondral bone and bone marrow, while on the surface of the transplant facing the joint cavity infiltrating cells were absent [ 50 , 52 ]. This suggested that immunisation of recipients occurred via the contact of chondrocytes with bone marrow cells.…”

Section: Immunogenic Properties Of Chondrocytes – Implications For Chmentioning

confidence: 99%

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Antigenic and immunogenic properties of chondrocytes. Implications for chondrocyte therapeutic transplantation and pathogenesis of inflammatory and degenerative joint diseases

Osiecka-Iwan¹,

Hyc²,

Radomska-Leśniewska³

et al. 2018

cejoi

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In physiological conditions chondrocytes are protected from contact with immunocompetent cells by the extracellular matrix, and transplanted fragments of allogeneic cartilage are not rejected. Cartilage produced by allogeneic chondrocytes, however, evokes the immune response of the recipient and is gradually destroyed. Immunisation by allogeneic chondrocytes is induced by the contact of their surface molecules with cells of the immune system. Chondrocytes constitutively express class I and, in some species, class II major histocompatibility complex (MHC) molecules. Expression of MHC class II molecules is induced in vitro by pro-inflammatory cytokines and in vivo in the course of the rejection of transplanted allogeneic cartilage. Low level of MHC class II molecules is found on the surface of human articular chondrocytes in patients with rheumatoid arthritis and osteoarthritis. Cartilage produced by transplanted allogeneic chondrocytes is destroyed by monocytes/macrophages and cytotoxic T and natural killer (NK) cells. NK cells show spontaneous cytotoxic reactivity against isolated chondrocytes and participate in the rejection of transplanted isolated chondrocytes. Chondrocytes express molecules that can serve as potential antigens in inflammatory joint diseases. Chondrocytes express cartilage-specific membrane antigen (CH65), human cartilage glycoprotein-39 (HC gp-39), hyaluronan binding adhesion molecule CD44, thymocyte antigen-1 (Thy-1) – CD90, signal transducer – CD24, lymphocyte function-associated antigen-3 (LFA-3) – CD58, and type I transmembrane protein Tmp21. On the other hand, although chondrocytes express major histocompatibility complex (MHC) class I and class II molecules, they can also exert immunosuppressive and immunomodulatory effects on immunocompetent cells. Isolated chondrocytes do not trigger an efficient allogeneic immune response in vitro and suppress, in a contact-dependent manner, proliferation of activated T cells. This suppression is associated with the expression by chondrocytes of multiple negative regulators of immune response. Chondrocytes express programmed death-ligand (PD-L), chondromodulin-I and indoleamine 2,3-dioxygenase (IDO), molecules that promote self-tolerance and suppress the immune system.

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Section: Immunogenic Properties Of Chondrocytes – Implications For Chmentioning

confidence: 99%

Section: Immunogenic Properties Of Chondrocytes – Implications For Chmentioning

confidence: 99%

Antigenic and immunogenic properties of chondrocytes. Implications for chondrocyte therapeutic transplantation and pathogenesis of inflammatory and degenerative joint diseases

Osiecka-Iwan¹,

Hyc²,

Radomska-Leśniewska³

et al. 2018

cejoi

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“…22,24,29,30 Giannini et al 29 hypothesized that this may be due in part to underappreciated immunogenicity of the allografts, which has been supported by basic science reasearch. [31][32][33] Others have argued that the size of the allograft tissue and mechanical factors lead to failure. 30 Patellofemoral instability is a source of these patellar and trochlear lesions, and the treatment of patellofemoral instability continues to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Bipolar Osteochondral Allograft Transplantation of the Patella and Trochlea

et al. 2018

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Objective To evaluate clinical, functional, and radiographic outcomes of patients who underwent bipolar osteochondral allograft transplantation (OCAT) of the patellofemoral joint (PFJ). Design Prospectively collected data on 18 knees who underwent fresh osteochondral allograft transplantation of the patella and trochlea by a single surgeon were reviewed. Inclusion criteria were: high-grade chondral lesions of PFJ (5 knees), or recurrent patella dislocations with trochlear dysplasia and chondral injury to the patella and/or trochlea (13 knees). Functional scores were obtained preoperatively and at follow-up appointments included Knee injury and Osteoarthritis Outcome Score (KOOS), International Knee Documentation Committee (IKDC), Oxford, Cincinnati, Tenger-Lysholm, visual analogue scale (VAS)-pain, and Single Assessment Numeric Evaluation (SANE). Grafts were also evaluated using Osteochondral Allograft MRI Scoring System (OCAMRISS). Results Three patients were lost to follow-up, leaving 4 knees in group 1, and 11 knees in group 2. Average age was 28.9 years (range 16-52 years). The average follow-up was 33.2 months (range 12-64 months). There was significant improvement of KOOS (from 38.7 to 83.1), IKDC (from 28.2 to 76.6), Tegner-Lysholm (from 38.3 to 88.3), Oxford (from 22.7 to 42.9), Cincinnati (from 35.1 to 83.6), VAS (from 71 to 17.9.), and SANE (from 43.3 to 83) ( P < 0.0001). The OCAMRISS score for patella was 2.23 and for trochlea 4.69. There were no revisions or conversions to arthroplasty Conclusion Bipolar OCAT of the patella and trochlea provide significant improvement in functional outcomes, relief from pain, activity level, and prevent recurrent instability.

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Section: Introductionmentioning

confidence: 99%

“…Therefore, a universal strategy needs to be established to overcome IRRs for allogenic cartilage regeneration in large animals and facilitate clinical translation of engineered cartilage. [11,12] Recently, a novel immunoisolation strategy using semipermeable membrane (SPM) materials has emerged as a feasible solution to protect allotransplanted cells and tissues, such as fibroblasts, mesenchymal stem cells, and islets, from immune attack by the host. [13,14] For variable tissue regeneration, it is essential to choose a proper SPM material for simultaneous efficient immunoisolation and stable tissue survival.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Stable Allogenic Cartilage Regeneration in a Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes

Huo

Bai

Zheng

et al. 2023

Adv Healthcare Materials

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Tissue engineering is a promising strategy for cartilage defect repair. However, autologous cartilage regeneration is limited by additional trauma to the donor site and a long in vitro culture period. Alternatively, allogenic cartilage regeneration has attracted attention because of the unique advantages of an abundant donor source and immediate supply, but it will cause immune rejection responses (IRRs), especially in immunocompetent large animals. Therefore, a universal technique needs to be established to overcome IRRs for allogenic cartilage regeneration in large animals. In the current study, a hybrid synthetic-natural electrospun thermoplastic polyurethane/gelatin (TPU/GT) semipermeable membrane to explore the feasibility of stable allogenic cartilage regeneration by an immunoisolation strategy is developed. In vitro results demonstrated that the rationally designed electrospun TPU/GT membranes has ideal biocompatibility, semipermeability, and an immunoisolation function. In vivo results further showed that the semipermeable membrane (SPM) efficiently blocked immune cell attack, decreased immune factor production, and cell apoptosis of the regenerated allogenic cartilage. Importantly, TPU/GT-encapsulated cartilage-sheet constructs achieved stable allogeneic cartilage regeneration in a goat model. The current study provides a novel strategy for allogenic cartilage regeneration and supplies a new cartilage donor source to repair various cartilage defects.

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Acute Rejection of Knee Joint Articular Cartilage in a Rat Composite Tissue Allotransplantation Model

Abstract: Immunorejection of chondrocytes in transplanted cartilage has been thought to be unlikely, but our data reveal that chondrocytes can undergo apoptosis in allotransplantation. This apoptosis involves the caspase-3 cascade and indicates that chondrocytes may induce acute rejection.

Cited by 8 publications

References 31 publications

Antigenic and immunogenic properties of chondrocytes. Implications for chondrocyte therapeutic transplantation and pathogenesis of inflammatory and degenerative joint diseases

Antigenic and immunogenic properties of chondrocytes. Implications for chondrocyte therapeutic transplantation and pathogenesis of inflammatory and degenerative joint diseases

Bipolar Osteochondral Allograft Transplantation of the Patella and Trochlea

In Vivo Stable Allogenic Cartilage Regeneration in a Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes

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