LIM and SH3 protein 1 localizes to the leading edge of protruding lamellipodia and regulates axon development

Pollitt, Stephanie L; Myers, Kenneth R.; Yoo, Jin San; Zheng, James

doi:10.1091/mbc.e20-06-0366

Cited by 7 publications

(6 citation statements)

References 68 publications

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“…We identified that filopodia are not the most abundant precursor but are the most efficient. Losing filopodia-initiated branches (in Plppr3 −/− neurons) appears to decrease the stability of the remaining branches, whereas a recent study has suggested that specifically losing lamellipodia results in a trend towards increased branch stability in a sample of only three neurons ( Pollitt et al, 2020 ). Furthermore, increasing filopodia-initiated branches by netrin-1 application increases the stability of branches.…”

Section: Discussionmentioning

confidence: 92%

Precursor types predict the stability of neuronal branches

Fuchs

Eickholt

2021

Journal of Cell Science

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Branches are critical for neuron function, generating the morphological complexity required for functional networks. They emerge from different, well-described, cytoskeletal precursor structures that elongate to branches. While branches are thought to be maintained by shared cytoskeletal regulators, our data from mouse hippocampal neurons indicate that the precursor structures trigger alternative branch maintenance mechanisms with differing stabilities. While branches originating from lamellipodia or growth cone splitting events collapse soon after formation, branches emerging from filopodia persist. Furthermore, compared to other developing neurites, axons stabilise all branches and preferentially initiate branches from filopodia. These differences explain the altered stability of branches we observe in neurons lacking the plasma membrane protein phospholipid phosphatase related protein 3 (PLPPR3/PRG2) or neurons treated with Netrin-1. Rather than altering branch stability directly, PLPPR3 and Netrin-1 boost a ‘filopodia branch program’ on axons, thereby indirectly initiating more long-lived branches. In summary, we propose that studies on branching should distinguish overall stabilising effects from effects on precursor types, ideally using multifactorial statistical models as exemplified in this study.

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Section: Discussionmentioning

confidence: 92%

Precursor types predict the stability of neuronal branches

Fuchs

Eickholt

2021

Journal of Cell Science

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“…We identified that filopodia are not the most abundant precursor, but the most efficient. Losing filopodia branches (in Plppr3 -/neurons) appears to decrease the stability of the remaining branches, while a recent study suggested that specifically losing lamellipodia trended towards increased branch stability in a sample of only three neurons (Pollitt et al, 2020). Both treatments appear to shift the equilibrium of branch precursor types, without affecting the stability of branches from each individual precursor.…”

Section: Branch Precursor Types Initiate Distinct Branching Systemsmentioning

confidence: 85%

Precursor types predict the stability of neuronal branches

Fuchs¹,

Eickholt²

2021

Preprint

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Branches are critical for neuron function, generating the morphological complexity required for functional networks. They emerge from different, well-described, cytoskeletal precursor structures that elongate to branches. While branches are thought to be maintained by shared cytoskeletal regulators, our data from mouse hippocampal neurons indicate that the precursor structures trigger alternative branch maintenance mechanisms with differing stabilities. While branches originating from lamellipodia or growth cone splitting events collapse soon after formation, branches emerging from filopodia persist. Furthermore, compared to other developing neurites, axons stabilise all branches and preferentially initiate branches from filopodia. These differences explain the decreased stability of branches we observe in neurons lacking the plasma membrane protein phospholipid phosphatase related protein 3 (PLPPR3/PRG2). Rather than altering branch stability directly, PLPPR3 boosts a ‘filopodia branch program’ on axons, thereby indirectly initiating more long-lived branches. We propose that studies on branching should distinguish overall stabilising effects from effects on precursor types, ideally using multifactorial statistical models as exemplified in this study.

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“…This KEGG pathway was enriched in upregulated genes, which revealed the importance of the cell cycle regulation and the replication process in paralarvae feed on M diet. Similarly, LIM and SH3 domain protein 1, a protein involved in neuronal differentiation and development (Phillips et al, 2004;Myers et al, 2020;Pollitt et al, 2020), and the PREDICTED: SAFB-like transcription modulator isoform X1, an RNA-DNA-binding protein, which plays crucial roles in DNA repair, processing of mRNA and regulatory RNA, and in the interaction with chromatin-modifying complexes (Norman et al, 2016), were also upregulated. Altogether, overrepresentation of these genes and proteins showed that a proper development and correct growth were occurring in paralarvae fed on M diet.…”

Section: Discussionmentioning

confidence: 99%

Proteogenomic Study of the Effect of an Improved Mixed Diet of Live Preys on the Aquaculture of Octopus vulgaris Paralarvae

et al. 2022

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The common octopus is the most demanded cephalopod species for human consumption. Despite important advances realized recently, the main bottleneck for commercial production of the common octopus, Octopus vulgaris, is the mass mortality of paralarvae in the first 15–20 days post-hatching (dph), with the main responsible factors still unknown. Thus, the identification of the limiting culture factors is, therefore, crucial for their aquaculture. This study investigates proteomic and transcriptomic responses of octopus paralarvae fed on an improved live preys-mixed diet (M) compared to an Artemia-based (A) reference diet. M diet resulted in the highest paralarvae specific growth rate obtained to date under culture conditions. This is supported by most of the proteins and genes over-expressed in the M group being linked to the cell cycle and replication, production of structural components, and development of the nervous system. Furthermore, the differential nutritional regulation of several genes and proteins seems to indicate that, instead of fatty acids, the preferred fuels for cephalopods would be proteins and carbohydrates. Also, M diet provides a better nutrient balance, which has allowed carrying out this comparative study in paralarvae under optimal conditions at a more advanced stage of growth (20 dph) than in previous studies. Moreover, the paralarvae culture extended up to 40 dph showed for the first time a proper pre-settlement behavior.

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LIM and SH3 protein 1 localizes to the leading edge of protruding lamellipodia and regulates axon development

Cited by 7 publications

References 68 publications

Precursor types predict the stability of neuronal branches

Precursor types predict the stability of neuronal branches

Precursor types predict the stability of neuronal branches

Proteogenomic Study of the Effect of an Improved Mixed Diet of Live Preys on the Aquaculture of Octopus vulgaris Paralarvae

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