Proteogenomic Study of the Effect of an Improved Mixed Diet of Live Preys on the Aquaculture of Octopus vulgaris Paralarvae

Abstract

The common octopus is the most demanded cephalopod species for human consumption. Despite important advances realized recently, the main bottleneck for commercial production of the common octopus, Octopus vulgaris, is the mass mortality of paralarvae in the first 15–20 days post-hatching (dph), with the main responsible factors still unknown. Thus, the identification of the limiting culture factors is, therefore, crucial for their aquaculture. This study investigates proteomic and transcriptomic responses of octopus paralarvae fed on an improved live preys-mixed diet (M) compared to an Artemia-based (A) reference diet. M diet resulted in the highest paralarvae specific growth rate obtained to date under culture conditions. This is supported by most of the proteins and genes over-expressed in the M group being linked to the cell cycle and replication, production of structural components, and development of the nervous system. Furthermore, the differential nutritional regulation of several genes and proteins seems to indicate that, instead of fatty acids, the preferred fuels for cephalopods would be proteins and carbohydrates. Also, M diet provides a better nutrient balance, which has allowed carrying out this comparative study in paralarvae under optimal conditions at a more advanced stage of growth (20 dph) than in previous studies. Moreover, the paralarvae culture extended up to 40 dph showed for the first time a proper pre-settlement behavior.

Insights into Common Octopus (Octopus vulgaris) Ink Proteome and Bioactive Peptides Using Proteomic Approaches

“…Glycolysis, the TCA cycle, the biosynthesis of nucleotide sugars, oxocarboxylic acid metabolism, and oxidative phosphorylation were the major metabolic pathways, and comparable results were obtained from previous ink gene ontology studies using DAVID and PANTHER. These metabolic networks were also identified through transcriptomics analysis in some previous studies [38][39][40]. Another significant iron soluble non-toxic protein ferritin was found in the metabolic networks of octopus ink, which is involved in the immune system and homeostasis process [41].…”

Identifying Natural Bioactive Peptides from the Common Octopus (Octopus vulgaris Cuvier, 1797) Skin Mucus By-Products Using Proteogenomic Analysis

“…The common octopus is a species with great food importance, since it is a complete food with great nutritional value and it is highly appreciated. Due to the high scientific interest and high commercial value of the common octopus, several transcriptomic and proteomic studies have been published, including adult specific organs [ 21 , 22 , 23 ] and paralarvae under different growth conditions [ 24 , 25 , 26 ]. However, the biological processes of this species are still not well understood due in part to this lack of genome sequencing and accurate annotation.…”

Efeito da temperatura no siri-azul Callinectes sapidus (BRACHYURA:PORTUNIDAE): aspectos fisiológicos, moleculares e proteômicos

“…A proteogenômica tem se destacado por permitir análises mais profundas de organismos não-modelos para inferir respostas fisiológicas a diversos estímulos endógenos e ambientais. Um estudo de proteogenômica realizado em larvas de Octopus vulgaris investigou a influência do enriquecimento alimentar no sucesso do seu cultivo através de 2.341 proteínas identificadas; no entanto, os autores destacam que a porcentagem de proteínas previstas, previstas/não caracterizadas e hipotéticas ultrapassa 75% do total revelando a alta complexidade de se trabalhar com dados massivos em organismos não-modelos (Varó et al, 2022). Nesta linha, Zhai e colegas (2021) realizaram uma análise proteogenômica usando um banco de dados personalizado com o objetivo de avaliar os mecanismos regulatórios do sistema imune do crustáceo Scylla paramamosain e demonstraram que 255 proteínas foram diferencialmente expressas após injeção de hormônios de vertebrados e que estavam envolvidas em vias de sinalização, imunidade celular, imunidade humoral e processos relacionados ao sistema imunológico.…”