Abstract:Osteoclast (OC) is the only cell involved in bone resorption. Dysfunction of OCs leads to a variety of bone diseases. Ligustilide (LIG) is the main component of the volatile oil isolated and purified from Angelica sinensis. LIG exerts many pharmacological activities, but its effects on osteoclastogenesis and bone resorption are still unclear. Our study showed that LIG inhibited receptor activator of nuclear factor‐κB (NF‐κB) ligand‐induced OC formation and activation in a dose‐dependent manner. Additionally, L… Show more
“…The histological results of the current study have strongly elucidated the bone regenerative capability of Angelica sinensis. This could be supported by various studies which proved that Angelica sinensis can inhibit wear debris particles-induced in ammatory bone resorption in mice [27] and can suppress osteoclastogenesis through inhibiting RANKL-mediated osteoclast differentiation in bone marrow macrophages decreasing bone resorption and preventing bone loss [28,29] .…”
Objective: Evaluating the effectiveness of Angelica sinensis, a widely used traditional Chinese medicine, in conjunction with Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in adult healthy mongrel dogs.
Materials and Methods: A randomized study design was conducted on the third and fourth premolars of six healthy mongrel dogs. A total of twenty-four class II furcation critical-sized defects were surgically created. After reflecting a fully-reflected mucoperiosteal flap, twelve defects were filled with Angelica sinensis powder mixed with Beta-tricalcium phosphate (β-TCP), then collagen membranes were applied over the defects (experimental group). The other twelve defects were filled with β-TCP followed by collagen membrane application (control group). The dogs were sacrificed at the end of fourth and eighth weeks. Jaw segments containing the operated teeth were dissected and processed for histological examination and morphometric analysis of the regenerative features.
Results: The histological results revealed that bone formation and regeneration of cementum and PDL progressed more in experimental groups than in control groups. Moreover, the regenerative features were more advanced in the second observation period. The histomorphometry revealed that the surface area of newly formed bone and the thickness of its trabeculae were higher in the defects treated with Angelica sinensis and β-TCP than in only β-TCP-filled defects at both observational periods.
Conclusions: Angelica sinensis has accelerated the regenerative potential of the interradicular bone and has enhanced the effect of β-TCP for interradicular bone regeneration in dogs.
“…The histological results of the current study have strongly elucidated the bone regenerative capability of Angelica sinensis. This could be supported by various studies which proved that Angelica sinensis can inhibit wear debris particles-induced in ammatory bone resorption in mice [27] and can suppress osteoclastogenesis through inhibiting RANKL-mediated osteoclast differentiation in bone marrow macrophages decreasing bone resorption and preventing bone loss [28,29] .…”
Objective: Evaluating the effectiveness of Angelica sinensis, a widely used traditional Chinese medicine, in conjunction with Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in adult healthy mongrel dogs.
Materials and Methods: A randomized study design was conducted on the third and fourth premolars of six healthy mongrel dogs. A total of twenty-four class II furcation critical-sized defects were surgically created. After reflecting a fully-reflected mucoperiosteal flap, twelve defects were filled with Angelica sinensis powder mixed with Beta-tricalcium phosphate (β-TCP), then collagen membranes were applied over the defects (experimental group). The other twelve defects were filled with β-TCP followed by collagen membrane application (control group). The dogs were sacrificed at the end of fourth and eighth weeks. Jaw segments containing the operated teeth were dissected and processed for histological examination and morphometric analysis of the regenerative features.
Results: The histological results revealed that bone formation and regeneration of cementum and PDL progressed more in experimental groups than in control groups. Moreover, the regenerative features were more advanced in the second observation period. The histomorphometry revealed that the surface area of newly formed bone and the thickness of its trabeculae were higher in the defects treated with Angelica sinensis and β-TCP than in only β-TCP-filled defects at both observational periods.
Conclusions: Angelica sinensis has accelerated the regenerative potential of the interradicular bone and has enhanced the effect of β-TCP for interradicular bone regeneration in dogs.
“…36 In addition, the TCR-CD3 complex contains the immune receptor tyrosine-activated motif (ITAM) domain, which can be mediated with MAPK and NF-kB signals to promote the expression of c-Fos and the differentiation, maturation, and proliferation of osteoclasts, leading to bone resorption. 37,38 (Please See Figure 11)Figure 11.Mechanisms of TCR signaling pathway in SLE and OP.…”
Objective This study aimed to explore the relationship between systemic lupus erythematosus (SLE) and osteoporosis (OP) based on bioinformatics. Methods The expression profiles of SLE and OP gene chips were searched through the GEO database, and the differentially expressed genes (DEGs) were screened out to obtain the intersection. Then, the Funrich software was used to predict the upstream miRNAs of the intersection genes, and the miRNA–mRNA relationship network was constructed. Afterward, the String database and Cytoscape software were used to construct the protein interaction network of the intersection genes to screen out the key genes. Finally, the functions and related pathways of key genes were analyzed by using the DAVID database. Results ①A total of 140 intersection genes of SLE and OP were obtained; ②There were 217 miRNAs regulating the intersection genes; ③IL-4, FOS, TLR1, TLR6, CD40LG, CCR1 were the key genes in the protein interaction network; ④The DAVID enrichment analysis mainly covered the positive regulation of cytokine production, the regulation of osteoclast differentiation, macrophage activation and other biological processes, involving Toll-like receptor signaling pathway, T cell receptor signaling pathway, Th1, Th2, and Th17 cells Differentiation, IL-17 signaling pathway. Conclusions SLE and OP still have some highly overlapping differential gene expressions under the background of complex gene networks. The gene functions and signaling pathways involved can simultaneously regulate the two diseases, suggesting that there is a close relationship between the molecular mechanisms of the two diseases, and that it may be a target of drugs that interfere with two diseases at the same time.
“…Angelica sinensis has medicinal effects on depression and cognitive disorders 4 , and its active ingredients include ligustilide (LIG) 5 , ferulic acid 6 , d -glucose 7 , etc. Among them, LIG is the main component of the volatile oil of angelica sinensis 8 , which has the properties of easy volatility and easy penetration of the blood-brain barrier, which allows LIG to be delivered to the brain through intranasal administration. Therefore, LIG has a high bioavailability in the treatment of brain diseases 9 , 10 .…”
Depression affects people with multiple adverse outcomes, and the side effects of antidepressants are troubling for depression sufferers. Aromatic drugs have been widely used to relieve symptoms of depression with fewer side effects. Ligustilide (LIG) is the main component of volatile oil in angelica sinensis, exhibiting an excellent anti-depressive effect. However, the mechanisms of the anti-depressive effect of LIG remain unclear. Therefore, this study aimed to explore the mechanisms of LIG exerting an anti-depressive effect. We obtained 12,969 depression-related genes and 204 LIG targets by a network pharmacology approach, which were intersected to get 150 LIG anti-depressive targets. Then, we identified core targets by MCODE, including MAPK3, EGF, MAPK14, CCND1, IL6, CASP3, IL2, MYC, TLR4, AKT1, ESR1, TP53, HIF1A, SRC, STAT3, AR, IL1B, and CREBBP. Functional enrichment analysis of core targets showed a significant association with PI3K/AKT and MAPK signaling pathways. Molecular docking showed strong affinities of LIG with AKT1, MAPK14, and ESR1. Finally, we validated the interactions between these proteins and LIG by molecular dynamics (MD) simulations. In conclusion, this study successfully predicted that LIG exerted an anti-depressive effect through multiple targets, including AKT1, MAPK14, and ESR1, and the pathways of PI3K/AKT and MAPK. The study provides a new strategy to explore the molecular mechanisms of LIG in treating depression.
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