Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids

Isikhuemhen, Omoanghe S.; Mikiashvilli, Nona A.

doi:10.1007/s10295-009-0620-1

Cited by 78 publications

(45 citation statements)

References 37 publications

(36 reference statements)

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“…This implies that hemicellulose was utilized more than cellulose by P. ostreatus. It has been reported that hemicellulose is preferentially utilized over cellulose during solid state fermentation by P. ostreatus [27,59,60]. Wang et al [61] also reported low degradation of cellulose by P. ostreatus cultivated on spent beer grains.…”

Section: Cellulose and Hemicellulosementioning

confidence: 99%

“…Tests on the potential use of 3 agricultural wastes for cultivating Pleurotus species [5] indicated that the colonization rate of peanut shell substrates was significantly lower than cotton waste or wheat straw. Reports have shown that P. ostreatus could potentially degrade single or combined lignocellulosic substrates [31,32], however degradation was enhanced in combined substrates [27,33,34]. …”

Section: Introductionmentioning

confidence: 99%

“…Pleurotus ostreatus, a white rot fungus, is primarily a lignocellulosic biomass decomposer. Several reports show that P. ostreatus can effectively colonize many lignocellulosic substrates [5,[26][27][28], preferentially degrade lignin and leave the bulk of cellulose and/or hemicellulose behind [29,30]. Tests on the potential use of 3 agricultural wastes for cultivating Pleurotus species [5] indicated that the colonization rate of peanut shell substrates was significantly lower than cotton waste or wheat straw.…”

Section: Introductionmentioning

confidence: 99%

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Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

FN¹,

Yusuf²

2016

J Bioremed Biodeg

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IntroductionPeanut shells (PS) are abundant agro-industrial waste products that are recalcitrant to degradation under natural conditions [1]. The increasing expansion of peanut production has led to accumulation of large quantities of these shells all over the world. In the US alone, peanut production increased by 45% between 2011 and 2012, bringing production to 3.04 million metric tons [2]. Globally, 45.6 million metric tons of peanuts are produced annually [3]. It has been estimated that for every kg of peanuts produced, 230-300 g of peanut shells are generated [4]. Therefore, as much as 13.7 million metric tons of peanut shells are churned out every year, most of which are dumped into the environment or burned [5,6]. Peanut shells are a renewable resource that could be targeted for purposeful use in the food, feed, paper and bioenergy industries. However, as yet, few if any value-added uses exist for them [7].Over the years, various attempts to utilize peanut shells have been made. Small fractions were incorporated into animal feed, especially for cattle [8], while some authors have experimented on their use as dietary fiber for humans [9]. Other non-food research trials include uses in pulp production [10], feedstock for bioethanol production [11,12], particle board component [13], mulch and activated charcoal [7]. However, the major drawback to their use in large scale industrial operations is the high lignin content.Much like other agricultural lignocellulolysic biomass, peanut shells are composed mainly of lignin, cellulose and hemicellulose. Lignin, a complex polymer, is the most recalcitrant component of plant biomass [14] and binds tightly to, and provides a physical seal around, cellulose and hemicellulose [11,[15][16][17][18], thereby protecting them against attack by hydrolytic enzymes. The restricted bioavailability of these sugars and other components is thought to be largely physical, with lignin molecules reducing the surface area available to enzymatic penetration and activity [19]. In order to gain access to the sugars in cellulose and AbstractWorld consumption of peanuts has increased tremendously, resulting in abundance of peanut shell waste. The high lignin content of peanut shells limits their bioconversion to useful products or recycling. Therefore, the synergy in co-substrating peanut shells (PS) and cornstalks (CS) to enhance biodegradation was evaluated. Various compositions of peanut shells and cornstalks (% dry weight) herein called co-substrate -90PS:10CS, 75PS:25CS, 50PS:50CS, 25PS:75CS, 10PS:90CS, and two controls Cont1-100PS and Cont2-100CS were studied under solid state fermentation (SSF) with a white rot fungus, Pleurotus ostreatus for 120 days. A two-factorial experiment in a completely randomized design (CRD) was used. Results showed that substrate composition and fermentation time were important variables in substrate degradation. Lignin degradation, losses in organic matter, cellulose and hemicellulose increased with time. Increasing the composition of CS in co-substrates...

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Section: Cellulose and Hemicellulosementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

FN¹,

Yusuf²

2016

J Bioremed Biodeg

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“…The residual lignocellulosic substrates can be reused by some microorganisms (Wu et al 2014) or hydrolyzed into the fermentable sugars (Balan et al 2008;Wu et al 2013). Extracellular enzymes from SMC include cellulases, xylanases, and ligninases (Isikhuemhen and Mikiashvilli 2009;MayoloDeloisa et al 2009;Yang et al 2015), the activities and amounts of which depend on the mushroom species and its cultivation conditions. These extracellular enzymes have shown wide applications in the brewing, baking, leather, textile, and saccharification industries (Xin and He 2013;Goncalves et al 2015;Kocabas et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Xylanase from Spent Mushroom Compost and its Application in Saccharification of Biomass Wastes

Lin

Sun

et al. 2017

BioResources

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The activities of xylanase extracted from spent mushroom composts (SMCs) of Coprinus comatus, Auricularia auricular, Pleurotus ostreatus, Pleurotus citrinopileatus, Agrocybe cylindracea, Hericium erinaceus, Hypsizygus marmoreus, and Tremella fuciformis were investigated. The crude extract from T. fuciformis SMC showed high xylanase activity with a value of 255.2 U/mg. Furthermore, this xylanase was purified using a combination of ammonium sulfate precipitation, diethylaminoethylcellulose (DEAE-cellulose), and gel filtration column chromatography. The enzyme was purified 20.7-fold with a yield of 43.1% and activity of 5293.8 U/mg. The purified xylanase showed maximum activity at 50 °C and pH 6, retained 80% activity after 1 h incubation at 50 °C, and sustained stability over a wide range of pH values (2 to 10). Under the optimal conditions, the enzyme exhibited a Km value of 2.5 mg/mL towards birchwood xylan. The activity of xylanase was enhanced in the presence of Mg 2+ , Ca 2+ , Ba 2+ , NH4 + , and Tween 80, while some metal ions, particularly Fe 3+ , inhibited its activity. The saccharification of several biomass wastes using the crude xylanase enzyme was studied. The results showed the potential for saccharification of alkaline-pretreated wheat bran solution where 75% saccharification was achieved.

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“…In addition, there is also another important reason why fungal is used as starter for organic fertilizer, that is the fungal ability to produce endogenous auxin hormone such as indol acetic acid (IAA) hormone (Ahmad et al 2005). Combination of lignocellulolytic enzyme and IAA hormone will produce organic fertilizer that is nutrition rich and containing high concentration of growth hormone regulator, which is necessary to increase the soil fertility and can accelerate the growth of roots, branches, bars, and leaves (Isikhuemhen and Mikiashvilli 2012 Pseudomonas putida (1.1225 μg mL ) (Levean and Lindow 2004;Ahmad et al 2005). Fungal isolates having high activity of lignocellulolytic enzyme and IAA hormone were characterized by measuring the biomass dry weight at varying temperature and pH.m Cultivation was performed on -1 shaker incubator (72 h, 100 rpm min ) with pH variation from 4.0 to 8.0 (with interval pH 1.0) and temperature variation between 20 °C and 60 °C (with interval temperature 10 °C).…”

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confidence: 99%

The Lignocellulolytic Activity and Ability to Produce Indole Acetic Acid Hormone of Fungal Inoculant Isolated From Spent Mushroom (Agaricus sp.) Substrate

Setiarto

Saskiawan

2013

Microbiol Indones

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Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids

Cited by 78 publications

References 37 publications

Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

Purification and Characterization of Xylanase from Spent Mushroom Compost and its Application in Saccharification of Biomass Wastes

The Lignocellulolytic Activity and Ability to Produce Indole Acetic Acid Hormone of Fungal Inoculant Isolated From Spent Mushroom (Agaricus sp.) Substrate

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