Light-Triggered Fluorescence Self-Reporting Nitric Oxide Release from Coumarin Analogues for Accelerating Wound Healing and Synergistic Antimicrobial Applications

Abstract

Nitric oxide (NO) has an important class of endogenous diatomic molecules that play a key regulatory role in many physiological and biochemical processes. However, the type of nitrosamine NO donor stimulated by light has many advantages compared to the conventional NO donors such as diazeniumdiolates and S-nitrosothiols compounds, including easy synthesis, good stability, and controllable release. In addition, NO release can be regulated by light induction with a built-in calibration mechanism fluorescence. Here, we report that the migration and proliferation of human umbilical vein vascular endothelial cells could be accelerated by the light-triggered NO donors, leading to the angiogenesis. Meanwhile, the screened NO donor 3a with Levofloxacin (Lev) showed synergistic effects to eradicate Methicillin-resistant Staphylococcus aureus (MRSA) biofilms in vitro and treat bacteria-infected wound in vivo.

A Novel Scaffold for Nitric Oxide Photo-Releasers based on Meso-Aminomethyl-BODIPY

“…Synthesis of NO photodonors Generally, there are no approaches to predict the efficiency of NO photorelease, and its relation to the molecular structure is not fully understood. Moreover, different mechanisms of NO release were reported, including intramolecular charge transfer (ICT) [34], photoinduced electron transfer (PET) [35], or homolythic fission with the formation of aminium radicals [36]. BODIPY-based N-NO photodonors reported previously [27,28] contain extended linker connecting BODIPY and NO-releasing moiety.…”

“…BODIPY-based N-NO photodonors reported previously [27,28] contain extended linker connecting BODIPY and NO-releasing moiety. On the other hand, N-NO group in several cases was directly attached to a chromophore [24,35]. We decided to use short methylene linkage between BODIPY and N-NO.…”

“…In several papers, the photorelease of NO was associated with the fluorescence turn-on [35,38]. It is explained by the fact that the presence of NO in the molecule leads to the photoinduced electron transfer (PET), which eventually leads to the N-N bond dissociation.…”

Ecofriendly one-pot fabrication of 7-methoxy coumarin encapsulated zeolitic imidazole nanocomposite for pH-responsive wound healing application and its biosafety evaluations

“…The formation of metal coumarin complexes plays an ideal role in biological activities. Recently, it has been reported that metal and hydroxycoumarin complexes might be applicable as anticoagulants, luminescent for DNA conformation, chemical sensors, and molecular devices [37][38][39][40]. N and S donor ligands greatly enhanced the coordination bonding with metal ions.…”

A cell membrane repair protein-based nanoformulation with multiple actuators for scarless wound healing

“…As one of the most prevalent chronic complications of diabetes, diabetic wounds are gaining widespread attention due to their difficulty of healing, and the etiology of this condition frequently results from persistent inflammation and cell dysfunction. 1 Wound repair is a complicated multifactor and multistep process that comprises phases of hemostasis, inflammation, proliferation, and remodeling, 2 and conventional therapies, including debridement, dressing changes and glucose-lowering treatment, do not seem to be effective in the treatment of diabetic wounds. However, newly developed drugs, including basic fibroblast growth factor and vascular endothelial growth factor, only apply to a specific phase of the wound repair process, and their therapeutic effects and clinical applications are also limited.…”