Light Sheet Microscopy to Measure Protein Dynamics

Rieckher, Matthias

doi:10.1002/jcp.25451

Cited by 7 publications

(5 citation statements)

References 102 publications

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“…This contrasts with other 3D imaging approaches such as confocal microscopy, where image acquisition is slow and the entire sample is illuminated for each optical section [ 120 ], thus making the light dose delivered to the sample prohibitively high. Light sheet microscopy has demonstrated its capability to capture the dynamics of small molecules; protein localization [ 121 ]; calcium ion signaling [ 122 ]; and oligonucleotide molecules within large, living organisms [ 123 ]. The application of light-sheet microscopy in the field of embryology is an emerging area of interest [ 124 ].…”

Section: Molecular-based Optical Imagingmentioning

confidence: 99%

Viewing early life without labels: optical approaches for imaging the early embryo

Chow,

Tan,

Upadhya

et al. 2024

Biology of Reproduction

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Embryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo quality rely on subjective morphology assessments or an invasive biopsy for genetic testing. However, both approaches can be inherently inaccurate and crucially, fail to improve the live birth rate following the transfer of in vitro produced embryos. Optical imaging offers a potential non-invasive and accurate avenue for assessing embryo viability. Recent advances in various label-free optical imaging approaches have garnered increased interest in the field of reproductive biology due to their ability to rapidly capture images at high-resolution, delivering both morphological and molecular information. This burgeoning field holds immense potential for further development, with profound implications for clinical translation. Here, our review aims to: 1) describe the principles of various imaging systems, distinguishing between approaches that capture morphological and molecular information, 2) highlight the recent application of these technologies in the field of reproductive biology, and 3) assess their respective merits and limitations concerning the capacity to evaluate embryo quality. Additionally, the review summarizes challenges in the translation of optical imaging systems into routine clinical practice, providing recommendations for their future development. Finally, we identify suitable imaging approaches for interrogating the mechanisms underpinning successful embryo development.

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Section: Molecular-based Optical Imagingmentioning

confidence: 99%

Viewing early life without labels: optical approaches for imaging the early embryo

Chow,

Tan,

Upadhya

et al. 2024

Biology of Reproduction

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“…Additionally, LSM can be easily combined with other modalities focusing on fluorescence, for instance, the coupling with fluorescence correlation microscopy to study the 3D distribution and movement of cytosolic proteins or the association with fluorescence lifetime imaging microscopy to analyze protein-protein interactions. Another remarkable advantage of LSM imaging systems is their simple design, allowing researchers to build an LSM apparatus themselves, specifically adapted to achieve personal objectives [110].…”

Section: High-resolution 3d Optical Mapping Of Intratumoral Nanoparti...mentioning

confidence: 99%

The Role of Optical Imaging in Translational Nanomedicine

Hesemans

Buttiens²,

Manshian³

et al. 2022

JFB

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Nanomedicines have been a major research focus in the past two decades and are increasingly emerging in a broad range of clinical applications. However, a proper understanding of their biodistribution is required to further progress the field of nanomedicine. For this, imaging methods to monitor the delivery and therapeutic efficacy of nanoparticles are urgently needed. At present, optical imaging is the most common method used to study the biodistribution of nanomaterials, where the unique properties of nanomaterials and advances in optical imaging can jointly result in novel methods for optimal monitoring of nanomaterials in preclinical animal models. This review article aims to give an introduction to nanomedicines and their translational impact to highlight the potential of optical imaging to study the biodistribution of nanoparticles and to monitor the delivery and therapeutic efficacy at the preclinical level. After introducing both domains, the review focuses on different techniques that can be used to overcome some intrinsic limitations of optical imaging and how this can specifically benefit nanoparticle studies. Finally, we point out some important key features of nanoparticles that currently hinder their full potential in the clinic and how the advances in optical imaging can help to provide us with the information needed to further boost the clinical translation and expand the field of nanomedicines.

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“…For instance, light sheet microscopy, which entails the use of a focused light sheet to illuminate the sample and the collection of the fluorescence signal via an orthogonally positioned imaging objective, significantly improves the signal-to-background ratio and is suitable for single-molecule imaging in a 3D space (117). With the low photobleaching and rapid optical sample sectioning, new combinations with light sheet microscopy will help researchers track and quantify the 3D distribution and movement of membrane and intracellular proteins (115). Moreover, super-resolution microscopy will facilitate the development of several new combined approaches, such as interferometric PALM and STED-FCS/FCCS, which further promote investigations of the spatiotemporal organization of protein complexes and interactions in plants.…”

Section: ) Husbands Et Al (55) Applied Simpull To Plants To Analyze T...mentioning

confidence: 99%

Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells

Wang

Xue

Xing

et al. 2018

Annu. Rev. Plant Biol.

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Plasma membrane proteins have important roles in transport and signal transduction. Deciphering the spatiotemporal organization of these proteins provides crucial information for elucidating the links between the behaviors of different molecules. However, monitoring membrane proteins without disrupting their membrane environment remains difficult. Over the past decade, many studies have developed single-molecule techniques, opening avenues for probing the stoichiometry and interactions of membrane proteins in their native environment by providing nanometer-scale spatial information and nanosecond-scale temporal information. In this review, we assess recent progress in the development of labeling and imaging technology for membrane protein analysis. We focus in particular on several single-molecule techniques for quantifying the dynamics and assembly of membrane proteins. Finally, we provide examples of how these new techniques are advancing our understanding of the complex biological functions of membrane proteins.

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Light Sheet Microscopy to Measure Protein Dynamics

Cited by 7 publications

References 102 publications

Viewing early life without labels: optical approaches for imaging the early embryo

Viewing early life without labels: optical approaches for imaging the early embryo

The Role of Optical Imaging in Translational Nanomedicine

Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells

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