Light Quality in Vitro Growth and Acclimatization of Two Varieties of Phalaenopsis Amabilis Alba Blume (Orchidaceae)

Spectral quality is an important factor for in vitro development of explants in a bioreactor system. Based on the need to optimize micropropagation for E. grandis × E. urophylla clones, the aim of the study was to assess the spectral quality of in vitro multiplication in temporary immersion bioreactor (TIB). The tissue used to generate the explants (i.e., the nodal segment with 1 cm of length and two axillary bud without leaves) was previously in vitro established and multiplied, it derived from ministumps of E. grandis × E. urophylla clone grown in a semi-hydroponic system. The spectral quality of in vitro multiplication was assessed through five light sources (i.e., fluorescent lamp, red, green, blue, and yellow cellophane). Morphological and anatomical features of tissues grown in TIB were evaluated at 90 days. Based on the results, yellow and blue spectral qualities were the most suitable to be adopted for in vitro multiplication of E. grandis × E. urophylla, since they enabled lesser hyperhydricity, favors high number of shoots per explant and shoot length, as well as thicker mesophyll and spongy parenchyma; arise as an alternative for large-scale production of eucalypts clonal plants.

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“…(Liu et al 2014), Populus euramericana Moench. (Kwon et al 2015) and Phalaenopsis amabilis alba Blume (Massaro et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

et al. 2020

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“…Light Emitting Diode (LED) have also been used in tissue culture to improve plantlet quality, as well as accelerate plant growth and improve rooting, which may be reflected in the high survival of plantlets in the acclimatization (Massaro et al 2018). LED light can act similarly to plant growth regulators (PGR) (Folta & Childers 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae)

et al. 2021

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Balanced levels of macronutrients and sucrose may ensure the success of micropropagation of the endangered ones. This study aimed to evaluate the effect of levels of salts in the culture medium on in vitro culture of Lomatozona artemisiifolia, as well as to determine the influence of light emitting diode (LED) on the shoot proliferation and rooting of the species. Nodal segments were used to evaluate the different macronutrient concentrations of MS medium (25, 50 and 100%), as well as sucrose concentrations (0.0 mM; 0.34 mM; 0.68 mM and 1.03 mM). Five light conditions were evaluated at shoots proliferation and rooting [100% blue (455 nm); 100% red (630 nm); 30% blue + 70% red; 30% red + 70% blue or fluorescent white]. Low levels of macronutrients in MS (50% and 25%) and sucrose (0.00 mM and 0.34 mM) resulted in plants with higher height, number of shoots and higher production of photosynthetic pigments. The 100% red light promoted rooting of 100% of the plants, and in 100% red or 70% red + 30% blue, higher plants were observed. These results demonstrate that under in vitro conditions, L. artemisiifolia has low nutritional needs, typical of plants that live in rupestrian fields.

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“…However, Cattleya crispata culture remains a challenge, since there are specific demands in the germination, growth, and developmental phases. The population's regeneration is compromised by slow growth and mutualistic interactions with insects and microorganisms, because of pollination or germination (MASSARO et al, 2018). Micropropagation is an alternative to the genetic conservation and multiplication of Cattleya crispata since thousands of plants can be produced from a single propagule.…”

Section: Introductionmentioning

confidence: 99%

THE INFLUENCE OF LIGHT QUALITY IN THE in vitro CULTIVATION OF Cattleya crispata

Souza

Fernandes

Molinari

et al. 2021

NAT

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Micropropagation technique is a valuable alternative for high quality genetic preservation of endemic species such as the orchid Cattleya crispata from “Campo Rupestre Ferruginoso”. This study aims to evaluate the influence of light quality on in vitro multiplication and elongation phases, offering new insights on the limiting factors of C. crispata. Seeds extracted from capsules were used for inoculation in the culture medium. Four light sources were evaluated for in vitro culture, namely: fluorescent lamp, white LEDs, red LEDs and red/blue LEDs. Data about the number of shoots, shoot length, shooting vigor and pigment content were assessed at 90 days of in vitro culture. Based on the recorded results, white LEDs are the most suitable ones for in vitro multiplication and elongation phases of C. crispata. It offers higher quality for seedling production and increases the chances of genetic conservation of the species. Keywords: ‘Campo Rupestre Ferruginoso’; in vitro propagation; wavelength; LEDs.

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Light Quality in Vitro Growth and Acclimatization of Two Varieties of Phalaenopsis Amabilis Alba Blume (Orchidaceae)

Cited by 7 publications

References 23 publications

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

Influence of macronutrients, sucrose and LED on in vitro culture of Lomatozona artemisiifolia (Asteraceae - Eupatorieae)

THE INFLUENCE OF LIGHT QUALITY IN THE in vitro CULTIVATION OF Cattleya crispata

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