Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla

Souza, Denys Matheus Santana Costa; Fernandes, Sérgio Bruno; Avelar, Maria Lopes Martins; Frade, Samira Rangel do Prado; Molinari, Letícia Vaz; Gonçalves, Douglas Santos; Pinto, José Eduardo Brasil Pereira; Brondani, Gilvano Ebling

doi:10.18671/scifor.v48n127.03

Cited by 9 publications

(11 citation statements)

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“…The irradiance of the two light bulbs combined (40 μmol m -2 s -1 ) was measured with a photoradiometer model QSO-S Procheck + Sensor-PAR Photon Flux (Decagon Devices, Pullman, Washington, USA). Sixty days after inoculation, percentage of tissue oxidation (Souza et al 2020b), shoot length per explant (cm), number of buds per explant, and photosynthetic pigment content [carotenoids, chlorophyll a, chlorophyll b, and total chlorophyll (a+b)] were evaluated.…”

Section: Plant Materials and In Vitro Multiplicationmentioning

confidence: 99%

“…Activation is dependent on genetic and epigenetic factors intrinsic to the species, as well as on their interaction for phenotypic expression (Gilmartin et al, 1990;He et al 2011), however, this activation begins with the stimulation of photoreceptors that signal metabolic pathways causing direct effects on tissue cell division and differentiation (Gilmartin et al 1990). Studies of different light spectra on the in vitro propagation of eucalypts species, provide important information to large-scale production of clones with high phytosanitary quality and high vigor (Souza et al 2020a;Souza et al 2020b).…”

Section: Introductionmentioning

confidence: 99%

“…Eucalypt micropropagation under different light qualities has been extensively studied showing positive results in plant health, inducing the increase of shoots and leaves (Souza et al 2020a;Miranda et al 2020), as well as seed germination (Rokich & Bell 1995). Besides, the use of cellophane plastic has shown to be a low-cost viable alternative as a filter for incident fluorescent light and providing the desired wavelength in micropropagation protocols (Souza et al 2020b).…”

Section: Introductionmentioning

confidence: 99%

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Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Frade

Souza

Fernandes

et al. 2023

NZJFS

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Background: In vitro growth and development of plants in the micropropagation stages are influenced by several factors, including the light spectral quality, which has shown important effects on the photomorphogenesis. The work aimed to evaluate the photomorphogenic effect of spectral qualities on in vitro culture of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla. Methods: Six light spectral qualities (i.e., red, white, blue, yellow, purple, and green) on in vitro multiplication, elongation, and adventitious rooting stages were evaluated through analysis of variance followed by a Tukey’s test. Results: White spectral quality was most adequate for in vitro multiplication of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, as it resulted in less tissue oxidation, longer shoot length, and more buds per explant. Red, blue and yellow spectral qualities increased the chlorophyll a, chlorophyll b, and total chlorophyll (a+b) leaf contents of Eucalyptus dunnii. To promote in vitro elongation, white spectral quality was most suitable for Eucalyptus dunnii, and yellow for Eucalyptus grandis × E. urophylla, as these resulted in more shoot length and shoots per explant. Red, white, blue and purple spectral qualities increased the stomatal density of Eucalyptus dunnii; while the white and yellow were the better for Eucalyptus grandis × E. urophylla. To promote in vitro rooting, the white and yellow spectral qualities caused the best results for the Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, with longer root length and more roots per explant. Eucalyptus dunnii showed reduced adventitious rooting, regardless of spectral quality. Conclusions: Light quality influence the morphophysiological responses of Eucalyptus in different stages of in vitro culture. Our results contribute to maximise the in vitro cloning of important eucalypts species.

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Section: Plant Materials and In Vitro Multiplicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Frade

Souza

Fernandes

et al. 2023

NZJFS

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“…The mean number of shoots per explant (> 0.5 cm), shoots length (> 0.5 cm), vigor based on the scores scale proposed by Souza et al (2020a) (Figure 1A-C) and the photo-synthetic ink content were assessed at 90 days of culture.…”

Section: Multiplicationmentioning

confidence: 99%

“…LEDs performance has increased the amount of chlorophyll and carotenoids and the photosynthetic growth ability of certain species (SOUZA et al 2020a). Carotenoids play an important role in photosynthesis since they protect the photosystem from photo-oxidation.…”

Section: Quality Of Light On In Vitro Elongationmentioning

confidence: 99%

THE INFLUENCE OF LIGHT QUALITY IN THE in vitro CULTIVATION OF Cattleya crispata

Souza

Fernandes

Molinari

et al. 2021

NAT

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Micropropagation technique is a valuable alternative for high quality genetic preservation of endemic species such as the orchid Cattleya crispata from “Campo Rupestre Ferruginoso”. This study aims to evaluate the influence of light quality on in vitro multiplication and elongation phases, offering new insights on the limiting factors of C. crispata. Seeds extracted from capsules were used for inoculation in the culture medium. Four light sources were evaluated for in vitro culture, namely: fluorescent lamp, white LEDs, red LEDs and red/blue LEDs. Data about the number of shoots, shoot length, shooting vigor and pigment content were assessed at 90 days of in vitro culture. Based on the recorded results, white LEDs are the most suitable ones for in vitro multiplication and elongation phases of C. crispata. It offers higher quality for seedling production and increases the chances of genetic conservation of the species. Keywords: ‘Campo Rupestre Ferruginoso’; in vitro propagation; wavelength; LEDs.

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