Light-microscopic studies of 3T3 cell plasma membrane alterations mediated by melittin

Lo, Wai Chun Jennifer; Henk, William G.; Enright, Frederick M.

doi:10.1016/s0041-0101(96)00082-7

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…It was noted that apamine and catecholamines such as noradrenaline (norepinephrine) and dopamine caused neurotoxic eOE ects and the degranulation of mast cells (Orlov 1979). Reports showed that melittin, a haemolytic peptide consisting of 26 amino acids, has eOE ects on erythrocytes and mast cells (Katsu et al 1990), leukaemic cells (Hait et al 1985 ;Killion & Dunn 1986), mouse ® broblast cells (Shier 1979 ;Lo et al 1997), skeletal muscle cells (Fletcher et al 1996 ;Ownby et al 1997), rat thymocytes (Duke et al 1994 ;Sakamoto et al 1996 ;Shaposhnikova et al 1997) and the human lymphoblastoid cell line (Weston & Raison 1998). Experiments showed that melittin was capable of binding to calmodulin, which plays a key role in cellular proliferation (Cheung 1982).…”

Section: Introductionmentioning

confidence: 99%

Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo

Liu

Chen

Xie

et al. 2002

Journal of Pharmacy and Pharmacology

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Bee venom has been reported to exhibit antitumour activity in-vitro and in-vivo. Apoptosis, necrosis and lysis of tumour cells were suggested as possible mechanisms by which bee venom inhibited tumour growth. The aim of this study was to investigate potential mechanisms by which bee venom inhibits K1735M2 mouse melanoma cells in-vitro and B16 melanoma, a transplantable solid melanoma in C57BL/6 mice, in-vivo. The proliferation of K1735M2 cells in-vitro was inhibited by bee venom in a concentration- and time-dependent manner. The inhibition was indicated by the arrest of the cell cycle at the G1 stage, as detected by flow cytometric measurements. The bee venom induced apoptosis-like cell death as identified by histological observations and by DNA fragmentation. In the in-vivo experiments, the bee venom (1.0, 3.0, 9.0 mg kg-1 of body weight, on days 1-12) was injected intraperitoneally into mice 24 h after the mice were inoculated with B16 cells. Inhibition of the solid tumour was observed. Apoptosis of the K1735M2 cells was suggested as the possible mechanism by which bee venom inhibited cell proliferation and induced K1735M2 cell differentiation in-vitro. The in-vivo experiment indicated that bee venom could be used as a chemotherapeutic agent against malignant tumours.

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Section: Introductionmentioning

confidence: 99%

Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo

Liu

Chen

Xie

et al. 2002

Journal of Pharmacy and Pharmacology

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“…As doses utilizadas em nosso experimento não permitiram a morte total dos cultivos celulares (CC 50 e CC 90 ), porém a rápida ação citolítica foi notada. Esta rápida ação citotóxica de melitina foi corroborada por diversos autores que realizaram estudos em diversos cultivos celulares (CERNE et al, 2013;LO et al, 1997;PRATT et al, 2005;SU et al, 2001;WESTON;RAISON, 1998). Estudos através de microscopia fluorescente demonstraram que linfócitos ovinos e humanos apresentaram permeabilidade da membrana celular em segundos de exposição à melitina em doses que variaram de 4,37 a 35 µM (SU et al, 2001).…”

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Sensibilidade De Linhagens Celulares Frente À Melitina Isolada De Veneno De Abelha

et al. 2017

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O estudo da composição e dos efeitos do veneno de abelha têm revelado efeitos farmacológicos, terapêuticos e antimicrobianos e estimulou a análise de seus constituintes de maneira isolada. Melitina, o principal componente da apitoxina, foi avaliada quanto à sua toxicidade frente a diferentes linhagens celulares. Para tanto, células MDBK, MDCK, CRFK, RK13 e Vero foram cultivadas e submetidas à exposição com melitina em concentrações que variaram de 1 a 10 µg/mL para obtenção das concentrações citotóxicas para 50% e 90% dos cultivos celulares (CC50 e CC90, respectivamente) através da avaliação de viabilidade celular pelo ensaio de redução do MTT. Cultivos foram preparados e tratados com as CC50 e CC90 e permaneceram expostos por diferentes períodos quando novamente tiveram sua viabilidade celular avaliada pelo mesmo método. As CC50 e CC90 (µg/mL) foram, respectivamente: MDBK (2,3 e 2,7), MDCK (2,8 e 3,2), CRFK (3,3 e 3,7), RK13 (4,1 e 4,7) e Vero (3,7 e 4,2). As células CRFK e Vero mostraram-se mais resistentes aos 5 minutos de exposição, visto que, na CC50 de melitina, apresentaram a mesma viabilidade celular em relação ao controle de células. Nos demais períodos avaliados, a viabilidade celular de todas as linhagens apresentou-se inferior ao controle de células (p<0,05), diminuindo a viabilidade em função do aumento do tempo de exposição e da concentração. Concluiu-se que a melitina apresentou toxicidade frente às linhagens celulares estudadas, dependente do tempo e da concentração.

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Melittin activates endogenous phospholipase D during cytolysis of human monocytic leukemia cells

Saini

Chopra

Peterson

1999

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Light-microscopic studies of 3T3 cell plasma membrane alterations mediated by melittin

Cited by 5 publications

References 10 publications

Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo

Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo

Sensibilidade De Linhagens Celulares Frente À Melitina Isolada De Veneno De Abelha

Melittin activates endogenous phospholipase D during cytolysis of human monocytic leukemia cells

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