Light-inducible control of cellular proliferation and differentiation by a Hedgehog signaling inhibitor

Misawa, Ryuji; Minami, Tsuyoshi; Okamoto, Akimitsu; Ikeuchi, Yoshiho

doi:10.1016/j.bmc.2021.116144

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Local expression of SHH has been demonstrated by using light-activatable Cre recombinase system [ 108 ]. Toward this purpose, light-activatable HH signaling modulator compounds have been developed [ 109 , 110 ].The first approach is to induce developmental body axes for generating multiple regions within an organoid along the axes. Interestingly, spontaneous formation of dorsoventral axis within an organoid has been achieved by culturing the organoid on a gel and exposing the organoid to SHH or equivalent compounds [ 111 ].…”

Section: Brain Organoid Engineeringmentioning

confidence: 99%

Advances in construction and modeling of functional neural circuits in vitro

Chow

Osaki

et al. 2022

Neurochem Res

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Over the years, techniques have been developed to culture and assemble neurons, which brought us closer to creating neuronal circuits that functionally and structurally mimic parts of the brain. Starting with primary culture of neurons, preparations of neuronal culture have advanced substantially. Development of stem cell research and brain organoids has opened a new path for generating three-dimensional human neural circuits. Along with the progress in biology, engineering technologies advanced and paved the way for construction of neural circuit structures. In this article, we overview research progress and discuss perspective of in vitro neural circuits and their ability and potential to acquire functions. Construction of in vitro neural circuits with complex higher-order functions would be achieved by converging development in diverse major disciplines including neuroscience, stem cell biology, tissue engineering, electrical engineering and computer science.

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