Advances in construction and modeling of functional neural circuits in vitro

Chow, Siu Yu A.; Hu, Huaruo; Osaki, Tatsuya; Levi, Timothée; Ikeuchi, Yoshiho

doi:10.1007/s11064-022-03682-1

Cited by 2 publications

(2 citation statements)

References 136 publications

(146 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For a single cell after subcellular chemical damage, the tentacle would lose its viability (shown by the calcein-AM test) without shrinkage or regeneration, while the cell body would heal its wound and regenerate (Figure e), which indicated that the nucleus might be essential for the survival, wound healing, and regeneration of neural cell fragments. These results showed that our design is an effective method for in situ subcellular stimulation, which could be potentially combined with neural models – and chemical analysis (e.g., subcellular mass spectrometry or microsensors) to promote neurology research.…”

Section: Results and Discussionmentioning

confidence: 87%

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

Zhang,

Xie,

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Confinement of chemical species in a controllable micrometer-level (several to a dozen micrometers) space in an aqueous environment is essential for precisely manipulating chemical events in subcellular regions. However, rapid diffusion and hard-to-control micrometer-level fluids make it a tough challenge. Here, a versatile open microfluidic method based on an aqueous two-phase system (ATPS) is developed to restrict species inside an open space with micron-level width. Unequal standard chemical potentials of the chemical species in two phases and space-time correspondence in the microfluidic system prevent outward diffusion across the phase interface, retaining the target species inside its preferred phase flow and creating a sharp boundary with a dramatic concentration change. Then, the chemical flow (the preferred phase with target chemical species) is precisely manipulated by a microfluidic probe, which can be compressed to a micron-level width and aimed at an arbitrary position of the sample. As a demonstration of the feasibility and versatility of the strategy, chemical flow is successfully applied to subcellular regions of various kinds of living single cells. Subcellular regions are successfully labeled (cytomembrane and mitochondria) and damaged. Healing−regeneration behaviors of living single cells are triggered by subcellular damage and analyzed. The method is relatively general regarding the species of chemicals and biosamples, which could promote deeper cell research.

show abstract

Section: Results and Discussionmentioning

confidence: 87%

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

Zhang,

Xie,

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Several other works stemmed from this original study [ 21 ] to explore some fundamental (and critical) steps for the development of neuromorphic neuroprostheses. The main elements of innovation are related to different types of communication modalities [ 56 ] by taking advantage of the latest technological developments [ 57 , 58 , 59 ] or increasing the complexity of the experimental model [ 60 ]: all the above comes with additional opportunities and challenges, as presented in the following sections.…”

Section: Neuromorphic Neuroprostheses: State-of-the-art and Perspectivesmentioning

confidence: 99%

Neuromorphic-Based Neuroprostheses for Brain Rewiring: State-of-the-Art and Perspectives in Neuroengineering

et al. 2022

Self Cite

View full text Add to dashboard Cite

Neuroprostheses are neuroengineering devices that have an interface with the nervous system and supplement or substitute functionality in people with disabilities. In the collective imagination, neuroprostheses are mostly used to restore sensory or motor capabilities, but in recent years, new devices directly acting at the brain level have been proposed. In order to design the next-generation of neuroprosthetic devices for brain repair, we foresee the increasing exploitation of closed-loop systems enabled with neuromorphic elements due to their intrinsic energy efficiency, their capability to perform real-time data processing, and of mimicking neurobiological computation for an improved synergy between the technological and biological counterparts. In this manuscript, after providing definitions of key concepts, we reviewed the first exploitation of a real-time hardware neuromorphic prosthesis to restore the bidirectional communication between two neuronal populations in vitro. Starting from that ‘case-study’, we provide perspectives on the technological improvements for real-time interfacing and processing of neural signals and their potential usage for novel in vitro and in vivo experimental designs. The development of innovative neuroprosthetics for translational purposes is also presented and discussed. In our understanding, the pursuit of neuromorphic-based closed-loop neuroprostheses may spur the development of novel powerful technologies, such as ‘brain-prostheses’, capable of rewiring and/or substituting the injured nervous system.

show abstract

Advances in construction and modeling of functional neural circuits in vitro

Cited by 2 publications

References 136 publications

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

Microfluidic Aqueous Two-Phase Focusing of Chemical Species for In Situ Subcellular Stimulation

Neuromorphic-Based Neuroprostheses for Brain Rewiring: State-of-the-Art and Perspectives in Neuroengineering

Contact Info

Product

Resources

About