Light-induced modulation of the mitochondrial respiratory chain activity: possibilities and limitations

Lunová, Mariia; Smolková, Barbora; Uzhytchak, Mariia; Janoušková, Klára Žofie; Jirsa, M; Egorova, Daria; Куликов, А. В.; Kubinová, Šárka; Dejneka, A.; Lunov, Oleg

doi:10.1007/s00018-019-03321-z

Cited by 30 publications

(57 citation statements)

References 97 publications

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“…This fact allows accurate fluorometric quantification of the percentage of metabolically active cells in the culture. We performed alamarBlue viability assay in accordance with the manufacturer's instructions and our verified treatment protocol [34]. Briefly, cells were seeded onto 96-well plates at the density of 5000 cells per well and treated with different concentration of nanoparticles for 24 h. After the treatment, alamarBlue reagent was added to each sample and incubated for 2 h at 37 • C. Fluorescence intensity (using excitation between 530 and 560 nm and emission at 590 nm) was measured using a TECAN microplate reader SpectraFluor Plus (TECAN, Mannedorf, Switzerland).…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…After cells were incubated with cell culture media (EMEM, 10% FBS) containing different concentrations of nanoparticles for 24 h. For lysosomal stability assessment, we utilized an acridine orange (AO) assay. The AO assay was performed in accordance with our previously verified protocol [6,34]. Briefly, cells with incorporated nanoparticles were labeled with 5 µg mL −1 AO in culture medium for 15 min at 37 • C. Following nanoparticle treatment, cells were cultured at 37 • C for indicated periods of time and the intensity of orange fluorescence was then measured using a microplate reader SpectraFluor Plus (TECAN, Mannedorf, Switzerland).…”

Section: Lysosomal Integrity Assaymentioning

confidence: 99%

“…It is worth noting here, that Huh7 cells express the highest level of p53 protein in comparison with Alexander and HepG2 cells [34,[92][93][94]. Therefore, we checked the sub-cellular distribution of p53 protein in Alexander, HepG2, and Huh7 cells.…”

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

“…In contrast, HepG2 cells show neither elevated mTOR endogenous phosphorylation (Figure 8c) nor nuclear p53 localization (Figure 8a,b) comparing to Huh7 and Alexander cells. However, HepG2 cells possess elevated levels of the anti-apoptotic Bcl-2 comparing to Huh7 and Alexander cells [34,94,97]. Taking into account the reported fluctuations concerning Bcl-2 expression in HepG2 cells in the literature [98,99], we analyzed Bcl-2 protein levels in all three cell lines.…”

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

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Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Uzhytchak

Smolková

Lunová

et al. 2020

Cells

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Iron oxide-based nanoparticles have been repeatedly shown to affect lysosomal-mediated signaling. Recently, nanoparticles have demonstrated an ability to modulate autophagic flux via lysosome-dependent signaling. However, the precise underlying mechanisms of such modulation as well as the impact of cellular genetic background remain enigmatic. In this study, we investigated how lysosomal-mediated signaling is affected by iron oxide nanoparticle uptake in three distinct hepatic cell lines. We found that nanoparticle-induced lysosomal dysfunction alters sub-cellular localization of pmTOR and p53 proteins. Our data indicate that alterations in the sub-cellular localization of p53 protein induced by nanoparticle greatly affect the autophagic flux. We found that cells with high levels of Bcl-2 are insensitive to autophagy initiated by nanoparticles. Altogether, our data identify lysosomes as a central hub that control nanoparticle-mediated responses in hepatic cells. Our results provide an important fundamental background for the future development of targeted nanoparticle-based therapies.

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Section: Cell Viability Assaymentioning

confidence: 99%

Section: Lysosomal Integrity Assaymentioning

confidence: 99%

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

See 2 more Smart Citations

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Uzhytchak

Smolková

Lunová

et al. 2020

Cells

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“…Thus, our next step was to evaluate the degree of intracellular ROS accumulation upon PMF treatment of SPION-loaded cells. We utilized a previously well-established methodology based on two distinct fluorescent probes [59][60][61][62]. One probe labels total ROS, while the other one is highly-specific against superoxide anion (O 2 − ).…”

Section: Triggering Apoptosis By Pmf Stimulation Via Disruption Of Lymentioning

confidence: 99%

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Lunov

Uzhytchak

Smolková

et al. 2019

Cancers

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Lysosome-activated apoptosis represents an alternative method of overcoming tumor resistance compared to traditional forms of treatment. Pulsed magnetic fields open a new avenue for controlled and targeted initiation of lysosomal permeabilization in cancer cells via mechanical actuation of magnetic nanomaterials. In this study we used a noninvasive tool; namely, a benchtop pulsed magnetic system, which enabled remote activation of apoptosis in liver cancer cells. The magnetic system we designed represents a platform that can be used in a wide range of biomedical applications. We show that liver cancer cells can be loaded with superparamagnetic iron oxide nanoparticles (SPIONs). SPIONs retained in lysosomal compartments can be effectively actuated with a high intensity (up to 8 T), short pulse width (~15 µs), pulsed magnetic field (PMF), resulting in lysosomal membrane permeabilization (LMP) in cancer cells. We revealed that SPION-loaded lysosomes undergo LMP by assessing an increase in the cytosolic activity of the lysosomal cathepsin B. The extent of cell death induced by LMP correlated with the accumulation of reactive oxygen species in cells. LMP was achieved for estimated forces of 700 pN and higher. Furthermore, we validated our approach on a three-dimensional cellular culture model to be able to mimic in vivo conditions. Overall, our results show that PMF treatment of SPION-loaded lysosomes can be utilized as a noninvasive tool to remotely induce apoptosis.

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Photobiomodulation in diabetic wound healing: A review of red and near‐infrared wavelength applications

Oyebode

Houreld

Abrahamse

2021

Cell Biochemistry & Function

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The development of a painless, non‐invasive, and faster way to diabetic wound healing is at the forefront of research. The complexity associated with diabetic wounds makes it a cause for concern amongst diabetic patients and the world at large. Irradiation of cells generates a photobiomodulatory response on cells and tissues, directly causing alteration of cellular processes and inducing diabetic wound repair. Photobiomodulation therapy (PBMT) using red and near‐infrared (NIR) wavelengths is being considered as a promising technique for speeding up the rate of diabetic wound healing, eradication of pain and reduction of inflammation through the alteration of diverse cellular and molecular processes. This review presents the extent to which the potential of red and NIR wavelengths have been harnessed in PBMT for diabetic wound healing. Important research challenges and gaps are identified and discussed, and future directions mapped out. This review thus provides useful insights and strategies into improvement of PBMT, including its acceptance within the global medical research community.

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Light-induced modulation of the mitochondrial respiratory chain activity: possibilities and limitations

Cited by 30 publications

References 97 publications

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Photobiomodulation in diabetic wound healing: A review of red and near‐infrared wavelength applications

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