Light-Induced H<sup>+</sup>Accumulation in the Vacuole of<i>Nitellopsis obtusa</i>

Gyenes, M.; Андрианов, В. К.; Булычев, А. А.; Kurella, G. A.

doi:10.1093/jxb/29.5.1185

Cited by 13 publications

(3 citation statements)

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“…The vacuolar pH is under metabolic control. When the cells of Valonia, Phaeoceros, and Nitellopsis were illuminated, the cell sap became more acid with concomitant changes in the vacuolar potential or the tonoplast potential (1,4,5). In isolated vacuoles of Tulipa, Hevea, and Saccharomyces, both the H+ accumulation and the membrane potential depend on MgATP (6,12,13,16,26).…”

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confidence: 99%

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Moriyasu

Shimmen

Tazawa

1984

Cell Struct. Funct.

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ABSTRACT. The vacuolar pH of Chara internodal cells was modified by vacuolar perfusion, and changes in membrane potential and membrane resistance of the tonoplast in response to the vacuolar pH (pHv) change were studied by anesthetizing the cell with 110 mM KCl in the external medium. Under this condition the responses of the vacuolar potential (Ev o) and resistance (Rvo) are assumed to reflect the responses of the tonoplast. E, o showed scarcely any sensitivity to the pHv when the KCl concentration of the vacuole ([KCl]v) was high (100 mM), but it changed by 30 mV in response to changes in the pHv from 5.5 to 7.5 when the [KCl]v was as low as 0.1 mM. This pHv sensitivity of Ev o was suppressed by DCCD, an inhibitor of H+-ATPase.Tonoplast vesicles were prepared from an internodal cell, and the effect of MgATP on their internal pHs was studied by estimating the accumulation of neutral red. A high accumulation of the pigment was found when MgATP was present in the external medium, but the color faded away when ATP was absent. These results together with the effect of DCCD on the Evo. indicate that there is an ATP-dependent electrogenic H+-pump in the tonoplasts of Chara internodal cells and that this electrogenicity is very small under normal conditions in which the natural cell sap contains about 100 mM KCl.Membrane characteristics of the tonoplast were compared with those of the plasmalemma with respect to their passive permeabilities to K+ and H+ and to the pump conductance.

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confidence: 99%

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Moriyasu

Shimmen

Tazawa

1984

Cell Struct. Funct.

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“…However, to uphold the findings of this study, further specification of the procedure is needed, since the degree of possible contamination among the main cell compartments remained unclear. For example, although vacuolar sap had been verified by its higher acidity (pH5.4–5.5) ( Gyenes et al, 1978 ) than that of cytoplasm, the presence of cytoplasm residues in the cell wall and/or vacuole compartments that could occur during the cell fractionation has not been taken into account. It is important to validate the cell fractionation procedure because charophyte cell is a suitable model for studying the localization of different toxicants and/or metabolites in the cell, and is of relevance for researchers working in plant cell physiology/toxicology ( Schwab et al, 2015 ; Lv, Christie & Zhang, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Biomarker identification of isolated compartments of the cell wall, cytoplasm and vacuole from the internodal cell of characean Nitellopsis obtusa

Gylytė

Jurkonienė

Cimmperman

et al. 2021

PeerJ

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Cells of characean algae are attractive for plant cell physiologists because of their large size and their close relation to higher plant cells. The objective of our study was to evaluate the purity of the compartments (cell wall, cytoplasm with plastids, mitochondria, nuclei and endomembrane system, and vacuole) separated mechanically from the internodal cells of Nitellopsis obtusa using enzymatic markers. These included α-mannosidase and malate dehydrogenase, vacuolar and cytoplasmic enzymes, respectively. The biomarkers applied revealed the degree of compartment contamination with the material from unwanted cell parts. The cell wall was contaminated slightly by vacuole and cytoplasm residuals, respectively by 12.3 and 1.96% of corresponding biomarker activities. Relatively high activity of vacuolar marker in the cell wall could be associated with the cell vacuoles in the multicellular structure of the nodes. The biomarkers confirmed highly purified vacuolar (99.5%) and cytoplasmic (86.7%) compartments. Purity estimation of the cell fractions enabled reevaluating nCuO related Cu concentrations in the compartments of charophyte cell. The internalisation of CuO nanoparticles in N. obtusa cell occurred already after 0.5h. In general, the approach seems to be useful for assessing the accumulation and distribution of various xenobiotics and/or metabolites within plant cell. All this justifies N.obtusa internodal cells as a model organism for modern studies in cell biology and nanotoxicology.

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“…Light-induced accumulation of H + in the vacuole of Nitellopsis obtusa has been observed. As the acidification of the vacuole is inhibited by 3-(3',4'-dichlorophenyl)-l,l-dimethylurea (DCMU), the H+-pumps in the tonoplast may also be activated through photosynthesis (Gyenes et al 1978). However, to the best of our knowledge, the regulation of the tonoplast H § has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina

Takeshige

Mitsumori

Tazawa

et al. 1992

Planta

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A unique variant strain of Chara corallina, which contains little inorganic phosphate in the vacuole ([Pi]v) was isolated. The level of cytoplasmic inorganic phosphate ([Pi]c) in these cells was the same as that in normal cells. Using these unique cells, we studied the change in [Pi]c and the effect of Pi on the activities of electrogenic H(+)-pumps associated with the plasma membrane and tonoplast. Upon illumination, the plasma membrane of C. corallina became hyperpolarized by 15 mV, the pH of the vacuolar sap decreased by 0.5 unit, and [Pi]c decreased by 30% with a similar time course. The activities of the electrogenic H (+)-pump in the plasma membrane and the ATP and PPi-dependent H(+)-transport in the tonoplast were noncompetitively inhibited by Pi with Ki values of, in the order given, 21.3 mM, 22.1 mM and 37.7 mM. From the kinetics study we calculated that the electrogenic H(+)-pump in the plasma membrane and the ATP and PPi-dependent H(+) transport in the tonoplast were activated by, again in this order, 13%, 13% and 9%, in accordance with the decrease in [Pi]c. We propose that the change in [Pi]c is one of the regulators of photosynthesis-mediated activation of the H(+)-pumps in the plasma membrane and the tonoplast in C. corallina upon illumination.

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Light-Induced H⁺Accumulation in the Vacuole ofNitellopsis obtusa

Cited by 13 publications

References 2 publications

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Biomarker identification of isolated compartments of the cell wall, cytoplasm and vacuole from the internodal cell of characean Nitellopsis obtusa

Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina

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Light-Induced H+Accumulation in the Vacuole ofNitellopsis obtusa

Cited by 13 publications

References 2 publications

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Electric characteristics of the vacuolar membrane of Chara in relation to pH regulation.

Biomarker identification of isolated compartments of the cell wall, cytoplasm and vacuole from the internodal cell of characean Nitellopsis obtusa

Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina

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Light-Induced H⁺Accumulation in the Vacuole ofNitellopsis obtusa