Light-Induced Changes in the Protein Pattern Translated in Vivo and in Vitro Accompanying Carotenogenesis in Neurospora crassa and Fusarium aquaeductuum

Mitzka-Schnabel, Ursula; Warm, E.; Rau, W.

doi:10.1007/978-3-642-69767-8_29

Cited by 10 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). We also found a similar stimulation after photoinduction in the fungi Nettroxpora and Fusarium following induction of caiotenogenesis (Mitzka-Schnabel et al 1984) and assume that this might be caused by a greater demand for specific enzymes.…”

Section: Discussionsupporting

confidence: 75%

Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction ofHyoscyamus niger

Warm¹

1984

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Flowering of the long day plant Hyoscyamus niger L., which is strictly photoperiodically controlled, was induced by 58 h continuous white light. The RNA from the leaves was isolated from photoperiodically induced and non‐induced plants and the poly(A)‐rich RNA separated by affinity chromatography on oligo‐dT‐cellulose. The poly(A)‐rich RNA was translated in vitro in the presence of 35S‐methionine using a rabbit reticulocyte lysate. Subsequent separation of the translation products by two‐dimensional polyacrylamide gel electrophoresis and fluorography allowed a comparison of the polypeptide pattern from induced and non‐induced leaf m‐RNA. The results indicate that induction of flowering is reflected by changes in the translation of several leaf polypeptides. These polypeptides were characterized by their isoelectric points and molecular masses.

show abstract

Section: Discussionsupporting

confidence: 75%

Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction ofHyoscyamus niger

Warm¹

1984

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

show abstract

“…These results have been independently confirmed with [2-14 C]MVA as the substrate (236). These results have been independently confirmed with [2-14 C]MVA as the substrate (236).…”

Section: F U N G Isupporting

confidence: 65%

“…The Site of Photoregulation. The levels of poly(A) RNA were higher in illuminated mycelia, and differences in the in vitro translated polypeptides were also de tected by two-dimensional electrophoresis (236). Evidence for the involvement of de novo mRNA synthesis has also been obtained from studies with Fusarium (302,303).…”

Section: F U N G Imentioning

confidence: 89%

Regulation of Carotenoid Biosynthesis

Bramley

Mackenzie

1988

Current Topics in Cellular Regulation

View full text Add to dashboard Cite

“…The low /3-galactosidase activity of the strain MR411 does suggest that transcription starts on the carA side of carB, in accord with these possibilities. Induction by blue light of the expression of specific genes has recently been reported in higher plants (17,18), but previously only indirect evidence has been found in microorganisms (19). Clearly, more experiments are required in M. xanthus to establish the regulatory mechanism suggested above for induction of gene expression by blue light.…”

Section: Resultsmentioning

confidence: 96%

Light induction of gene expression in Myxococcus xanthus

Balsalobre

Ruiz‐Vázquez

Murillo

1987

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The synthesis of carotenoids by Myxococcus xanthus requires illumination with blue light. Mutations at two loci (carA and carR) remove the blue-light requirement and cause constitutive production of carotenoids. Mutations at a different locus (car@) prevent carotenogenesis in both wild-type and constitutive mutant strains. We describe here three independent car mutations induced by insertion of Tn5 kac, a transposon that carries a transcriptional probe for exogenous promoters. All three transposon insertions block carotenogenesis even in constitutive mutant strains. One insertion is in a previously unknown cargene and the other two are in the carB locus. One ofthe carB insertions expresses -galactosidase at very low levels in the dark but is strongly activated by light. When this TnS. Iac insertion is introduced in carA or carR mutants it expresses -galac se in dark-as well as light-grown cells. We conclude that carotenogenesis in M. xanthus is activated at the level of transcription by a light-induced mechanism in which the carA and the carR loci (or their gene products) take part. The potential usefulness ofM. xanthus as a simple and sensitive tool for studies in photobiology is discussed.OD K1910 tcarR2) aoK2836(carB1)

show abstract

Light-Induced Changes in the Protein Pattern Translated in Vivo and in Vitro Accompanying Carotenogenesis in Neurospora crassa and Fusarium aquaeductuum

Cited by 10 publications

References 10 publications

Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction ofHyoscyamus niger

Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction ofHyoscyamus niger

Regulation of Carotenoid Biosynthesis

Light induction of gene expression in Myxococcus xanthus

Contact Info

Product

Resources

About