Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction of<i>Hyoscyamus niger</i>

Warm, E.

doi:10.1111/j.1399-3054.1984.tb06338.x

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…Under noninductive treatments, these proteins were not found. Warm (29), using in vitro translation of isolated mRNA and two-dimensional gel electrophoresis, found both qualitative and quantitative differences in translation products between induced and noninduced Hyoscyamus leaves. However, because different photoperiods were used in both of these comparisons, it is difficult to conclude that the changes were specifically related to floral induction.…”

Section: Discussionmentioning

confidence: 99%

Protein Differences between Two Isogenic Cultivars of Barley (Hordeum vulgare L.) that Differ in Sensitivity to Photoperiod and Far-Red Light

Principe

Hruschka²,

Thomas³

et al. 1992

Plant Physiol.

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A photoperiodically sensitive cultivar of barley (Hordeum vulgare L. Shabet) and an isogenic, single-gene recessive mutant of this genotype that is insensitive to photoperiod (BMDR-1) were grown under continuous cool white light with or without supplemental far-red fluorescent light. BMDR-1 initiates flowers 6 days after germination, irrespective of light treatment, whereas BMDR-8 remains vegetative for at least a week longer, even in continuous light. When far-red light is added, the delay of flowering in BMDR-8 is overcome and both genotypes initiate floral primordia at the same time. Total phenol extracted proteins of seedlings of both genotypes were resolved by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No protein differences were found between the genotypes when isoelectric focusing gels were run in the first dimension. Two qualitative genotypic differences were found when nonequilibrium pH gradient gel electrophoresis was run in the first dimension. An 85-kilodalton polypeptide (A) and a 26-kilodalton polypeptide (B) were always present in BMDR-8 but never found in BMDR-1. The levels of A appeared to decrease from the BMDR-8 during the first 3 days of far-red treatment but did not disappear completely even after 6 days of growth in the presence of farred. Polypeptide B decreases rapidly in continuous cool white light but is stabilized by far-red. The phytochrome content of BMDR-1 was found to be greater than that for BMDR-8. This increase appears to be caused by the type I (etiolated-tissue abundant) phytochrome pool, even in plants grown in continuous light.Despite the fact that the control of flowering by daylength has been intensively investigated for more than 70 years (13), the fundamental cellular and biochemical basis for this regulation remains unresolved. Nevertheless, photoperiodicallysensitive plants provide an important experimental tool that can be used to manipulate the onset of floral induction. The

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Section: Discussionmentioning

confidence: 99%

Protein Differences between Two Isogenic Cultivars of Barley (Hordeum vulgare L.) that Differ in Sensitivity to Photoperiod and Far-Red Light

Principe

Hruschka²,

Thomas³

et al. 1992

Plant Physiol.

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“…Warm (1984) observed changes in the composition of leaf mRNA after floral induction of the long-day (LD) plant Hyoscyamus niger. At least one of the new mRNAs appearing in the pattern after inductive conditions could again be seen upon chemoinduction of flowering in Hyoscyamus with the base analogue 5-thiouracil (Hofmann 1987).…”

Section: Introductionmentioning

confidence: 96%

A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Lechner

Rau

1993

Planta

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Abstract. Previous studies have indicated that the photoperiodic induction of flowering results in qualitative changes in gene expression in the leaves of the induced plant. However, we obtained different results when leaf tissue was examined at different sampling times after flower induction, thus pointing to the possibility that alterations in gene expression during photoperiodic induction are only transient. We analyzed the composition of mRNA populations in the leaves of the long-day plant Arabidopsis thaliana (L.) Heynh. at various times during photoperiodic induction. Polysomal mRNA populations were assayed during illumination programs consisting of different photoperiodic cycles. Quantitative changes of distinct leaf mRNAs were observed during the time course. The expression levels of most of these mRNAs oscillated in a diurnal or circadian rhythm with respect to the photoperiod. However, no novel mRNA appeared or decreased to undetectable levels during the changes in photoperiod. After the onset of inducing photoperiods, alterations in the phase and amplitude of these oscillations were seen. Various types of oscillation pattern were observed. These changes occurred either very rapidly, within hours after the additional light period began or appeared after the first altered photoperiod was complete. The majority of the changes seen at the beginning of inductive photoperiods were transient and the mRNA levels reverted to the original pattern when the plant was returned to non-inductive photoperiods. In addition, some of these alterations were found to be transient, irrespective of the length of subsequent light periods.

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“…In studies in which the effect of darkness on mRNA levels has been studied, only a single time following the transition to darkness has been examined (Warm, 1984;Lay-Yee et al, 1987;Ono et al, 1988;Bassett et al, 1991). In this study, we examined the pattem of mRNA abundance in the light and dark for up to 48 h and obtained a detailed analysis of the temporal pattem of expression during darkness for a number of Pharbitis genes.…”

Section: Ldentification Of Mrnas Associated With Photoperiodicmentioning

confidence: 99%

Dark and Circadian Regulation of mRNA Accumulation in the Short-Day Plant Pharbitis nil

O’Neill

Zhang²,

Zheng³

1994

Plant Physiol.

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Changes in the composition of in vitro translated leaf m‐RNA caused by photoperiodic flower induction ofHyoscyamus niger

Cited by 13 publications

References 40 publications

Protein Differences between Two Isogenic Cultivars of Barley (Hordeum vulgare L.) that Differ in Sensitivity to Photoperiod and Far-Red Light

Protein Differences between Two Isogenic Cultivars of Barley (Hordeum vulgare L.) that Differ in Sensitivity to Photoperiod and Far-Red Light

A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Dark and Circadian Regulation of mRNA Accumulation in the Short-Day Plant Pharbitis nil

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