Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition

Sáez, Patricia L.; Bravo, León A.; Latsague, Mirtha; Toneatti, Marcelo; Sánchez‐Olate, Manuel; Ríos, Darcy

doi:10.1016/j.plantsci.2012.11.008

Cited by 35 publications

(18 citation statements)

References 51 publications

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“…Usually, in vitro culture is carried out in chambers with low photon flux density (PFD), which may limit development of efficient photosynthetic and photoprotective mechanisms [6] [7]. Mechanisms such as heat dissipation and photochemical process that drain the excess electrons accumulated in the inter-system pool allow managing the excess of absorbed light energy [8] [9].…”

Section: Introductionmentioning

confidence: 99%

“…In vitro culture of C. sativa has allowed obtain a large number of individuals and solve the difficulties observed during the traditional macropropagation, due to the recalcitrant condition of this species. Despite significant advances in its micropropagation, and the studies conducted in order to increase the ex vitro survival rates [6] [7] [19], the effect of the sugars added to the culture medium of has not been evaluated. Based on this, the objective of this study was to evaluate the effect of exogenous sucrose, under different light conditions, on the photosynthetic performance and growth of Castanea sativa microshoots.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during In Vitro Culture of Castanea sativa

Sáez¹,

Bravo²,

Sánchez‐Olate³

et al. 2016

AJPS

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The low photon flux density (PFD) under in vitro conditions and sucrose added to the culture medium negatively limits the photochemical activity and photoprotective mechanisms of microshoots. In this work we hypothesize that decreasing sucrose in the culture medium in combination with increasing irradiance, could improve the photosynthesis and consequently the in vitro growth. We evaluated the effect of exogenous sucrose (30 and 5 g•L −1 , HS and LS, respectively), under different PFD (50 and 150 µmol photons m −2 •s −1 , LL and HL, respectively) on the photosynthetic performance and growth of Castanea sativa microshoots. Decreasing sucrose negatively affected the physiological attributes evaluated. Only chloroplast ultrastructure was improved by LS; however this did not lead to an improved in photosynthesis or growth. HL HS produced an increase in photosynthetic activity and chlorophyll contents, reaching under these conditions a higher proliferation rate and biomass production. Additionally, the photochemical activity (electron transport rate and nonphotochemical quenching) was improved by HL. Thus, our results suggest that, at least for C. sativa HL is beneficial during the in vitro culture, improving photosynthetic performance as well as growth, but this is only possible in the presence of moderate concentrations of sucrose added to the culture medium.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during In Vitro Culture of Castanea sativa

Sáez¹,

Bravo²,

Sánchez‐Olate³

et al. 2016

AJPS

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“…A commonly response to high light is the increment in xanthophyll pool size (VAZ), allowing them manage the excess of absorbed light by means of heat dissipation [41] [42]. Together, these parameters could determine a greater ability to withstand the stress of transfer to ex vitro conditions [31] and may therefore be an indicator of greater potential physiological performance once a micrograft coming from basal section in the ortet is consolidated. Unfortunately, the low efficiency of micrografting of these basal scions is a big constraint for a reinvigoration program.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, [30] reported higher photo-synthesis rates in juvenile and rejuvenated than adult in vitro grown Sequoia sempervirens shoots. Photosynthetic performance has also been positively correlated with growth rates and survival of in vitro plants and with their viability to establish in ex vitro conditions [31] and has been used as an indicator of better in vitro acclimatization treatments [26]. We hypothesize that variations in fluorescence parameters such as Fv/Fm, ETR and NPQ are good indicators of micrograft's viability and phenological stage.…”

Section: Introductionmentioning

confidence: 99%

Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

Materán¹,

Sáez²,

Sánchez‐Olate³

et al. 2015

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How to cite this paper: Materán, M.E., et al. (2015) M. E. Materán et al. 229lower Fv/Fm than apical shoot tips, which agrees with the lowest viability (35%). However, during the consolidation phase, the trend changed and basal shoot tips presented higher Fv/Fm than apical shoot tips and showed an increase in ETR and NPQ, with respect to apical shoots and ortet. Although the measurement of fluorescence parameters implies the insertion of the fluorometer sonde in vitro, this implies aseptic considerations, but always conveies a contamination risk. We conclude that fluorescence (Fv/Fm, ETR, NPQ) can be indicators of the micrograft's development according to the shoot tips position in the ortet and can be useful early-indicators of the scions' physiological condition during micrograft transition from establishment to consolidation.

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“…Pigments chosen for analysis included chlorophyll a and b , β-carotene (β-car), neoxanthin, violaxanthin, antheraxanthin, and zeaxanthin. Pigments were measured using the HPLC method described by García-Plazaola and Becerril (1999) with modifications included in Sáez et al (2013). De-epoxidation state (DEPS) of the xanthophyll pool was calculated as follow: DEPS = (V + 0.5A)/(V + A + Z), where V is violaxanthin, A is antheraxanthin, and Z is zeaxanthin.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Supply Affects Photosynthesis and Photoprotective Attributes During Drought-Induced Senescence in Quinoa

et al. 2018

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Drought during senescence has become more common in Mediterranean climates in recent years. Chenopodium quinoa Willd has been identified as tolerant to poor soil conditions and drought. Previous observations have found that sufficient nitrogen (N) supply mitigates yield losses under terminal drought conditions. However, there is no understanding of the mechanisms behind this effect. We hypothesized that N up-regulates both photosynthetic and photoprotective elements during drought-induced senescence, alleviating the negative impact of drought on yield. The role of N supply and terminal drought on photoprotection was tested using three Chilean quinoa genotypes from different climatic zones: Faro, UdeC9, and BO78. Plants were grown under high nitrogen (HN) or low nitrogen (LN) conditions and subjected to terminal drought at the onset of senescence. Photosynthetic and photochemical and non-photochemical processes were evaluated at both the onset of drought and after 15 days of drought conditions. N supplementation modified most of the physiological parameters related to photochemical dissipation of energy, photosynthesis, and yield in quinoa. In contrast, water restriction did not affect photosynthesis in quinoa, and its effect on yield was dependent on the genotype. A significant interaction N × G was observed in photosynthesis, relative water content, protein content, Fv/Fm, and chlorophylls. In general, Faro was able to maintain higher levels of these attributes under LN conditions than UdeC9 and BO78. In addition, the interacting effects of N × W regulated the level of most pigments in quinoa as well as the photoprotective induction of non-photochemical quenching (NPQ) during senescence. During terminal drought at LN conditions, Faro presented a larger NPQ induction under drought conditions than UdeC9 and BO78, which was supported by a larger zeaxanthin content and de-epoxidation state of the xanthophyll pool. Interestingly, BO78 did not induce NPQ in response to drought-induced senescence but instead enhanced the content of betacyanins. This response needs to be researched in future works. Finally, we observed that LN supply reduced the correlationship between the de-epoxidation state of the xanthophyll cycle and NPQ. This could be an indication that N supply not only compromised the capacity for photosynthetic performance in quinoa plants, but also affected the plasticity of thermal dissipation, restricting further changes during drought-induced senescence.

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Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition

Cited by 35 publications

References 51 publications

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during <i>In Vitro</i> Culture of <i>Castanea sativa</i>

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during <i>In Vitro</i> Culture of <i>Castanea sativa</i>

Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

Nitrogen Supply Affects Photosynthesis and Photoprotective Attributes During Drought-Induced Senescence in Quinoa

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Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition

Cited by 35 publications

References 51 publications

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during &lt;i&gt;In Vitro&lt;/i&gt; Culture of &lt;i&gt;Castanea sativa&lt;/i&gt;

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during &lt;i&gt;In Vitro&lt;/i&gt; Culture of &lt;i&gt;Castanea sativa&lt;/i&gt;

Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

Nitrogen Supply Affects Photosynthesis and Photoprotective Attributes During Drought-Induced Senescence in Quinoa

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Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during <i>In Vitro</i> Culture of <i>Castanea sativa</i>

Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during <i>In Vitro</i> Culture of <i>Castanea sativa</i>