Light-emitting diode technologies for TB diagnosis: what is on the market?

Minion, Jessica; Sohn, Hojoon; Pai, Madhukar

doi:10.1586/erd.09.26

Cited by 42 publications

(30 citation statements)

References 12 publications

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“…This fi nding is in accordance with previous reports. [16][17][18] Apart from its diagnostic effi ciency, LED-FM has several technical and economical benefi ts. 16,19 These features make LED technology a user-friendly and feasible alternative that can be implemented in resource-poor settings.…”

Section: Discussionmentioning

confidence: 99%

“…[16][17][18] Apart from its diagnostic effi ciency, LED-FM has several technical and economical benefi ts. 16,19 These features make LED technology a user-friendly and feasible alternative that can be implemented in resource-poor settings. 8,16,19 The highest increment was observed by bleach centrifugation, but compared to direct auramine method the difference was not statistically signifi cant.…”

Section: Discussionmentioning

confidence: 99%

“…16,19 These features make LED technology a user-friendly and feasible alternative that can be implemented in resource-poor settings. 8,16,19 The highest increment was observed by bleach centrifugation, but compared to direct auramine method the difference was not statistically signifi cant. The lack of an appreciable gain from the bleach method might be explained by the fact that under optimal conditions (i.e., appropriate specimen collection and smear preparation), the performance of direct auramine was good.…”

Section: Discussionmentioning

confidence: 99%

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Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Habtamu

Boogaard²,

Ndaro³

et al. 2012

int j tuberc lung dis

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Habtamu

Boogaard²,

Ndaro³

et al. 2012

int j tuberc lung dis

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“…Compared to conventional mercury vapour fluorescence microscopes, LED microscopes are less expensive and have lower maintenance requirements. The diodes are very durable, do not require warm-up time, and do not contain toxic products [8]. Importantly, they are reported to perform equally well without a darkroom.…”

Section: Introductionmentioning

confidence: 95%

Comparative Analysis of Performance of PREVI Fluo TB Method by Using LED-Microscopy

Solomon¹

2015

J Med Microb Diagn

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There is a need for rapid, practical and accurate tuberculosis diagnostic tools that are adapted to resource-poor settings in order to ensure that those affected receive proper and timely treatment. Light-Emitting-Diode microscopy (LED) has recently been endorsed by the WHO for diagnosis of TB in these countries. A much recent smear diagnosis method, PREVI Fluo TB by using LED microscopy, has been made available. However, due to the novelty of the method, there is no information available on the specificity and sensitivity when compared to established methods such as the ordinary LED-Auramine O or the classical widely used ZiehlNeelsen (ZN) in TB positive persons. This study was undertaken to compare the sensitivity, specificity, positive predictive value and negative predictive value of PREVI Fluo TB stain with ZN method and Auramine O-LED microscopy taking culture as reference. A prospective cross sectional study was conducted in St. Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia. SpotMorning-Spot sputum samples from 248 TB suspected study participants were collected. The smear detection rate of PREVI Fluo TB, ZN, Auramine O and culture were found to be 35 (14.1%), 24(9.7%), 44(17.7%) and 30(12.1%) respectively. The sensitivity of the PREVI Fluo TB method was 76.67%, better than ZN and slightly less than Auramine O which were 59.07% and 78.13% respectively. Nevertheless, the specificity (94.5%) was lower than ZN (96.79%) and higher than Auramine O (91.2%). The negative likelihood ratio of PREVI Fluo TB, ZN, and Auramine O methods were 0.25, 0.45, and 0.24 respectively. The PREVI Fluo TB method had a PPV of 65.71% which is higher than the Auramine O (56.82%) but with a comparable NPV (96.71% and 96.57% respectively). The respective agreements of the ZN, Auramine O and PREVI Fluo TB methods with the gold standard were K=0.585, K=0.621 and K=0.664. There was a substantial agreement of PREVI Fluo TB result with ZN (k=0.636) and Auramine O methods (K=0.745).Given the practical benefits of PREVI Fluo TB for TB diagnosis, and comparable accuracy to the current standard of Auramine O fluorescence method and the gold standard culture, PREVI Fluo TB should be considered by TB diagnostic laboratories, as an alternative diagnostic tool for conventional Auramine O fluorescent stain.

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“…This substitution increases the sensitivity of the test and is easy to use, even in peripheral laboratories where culture facilities are not available (Hooja et al, 2011;Steingart et al, 2006a;Steingart et al, 2006b;Steingart et al, 2007;Van Deun et al, 2008;Trusov et al, 2009;Minion et al, 2009;. In fact, the World Health Organization (WHO) Strategic and Technical Advisory Group (STAG) for TB recommended that fluorescence microscopy be phased in as an alternative for ZN (WHO, 2009), because it can be used even in low-income, high TB burden settings.…”

Section: Sputum Smear Microscopymentioning

confidence: 99%

Diagnosis of Mycobacterium tuberculosis

Al-Zamel¹

2012

Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis

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Light-emitting diode technologies for TB diagnosis: what is on the market?

Cited by 42 publications

References 12 publications

Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Comparative Analysis of Performance of PREVI Fluo TB Method by Using LED-Microscopy

Diagnosis of Mycobacterium tuberculosis

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