Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Habtamu, Meseret; Boogaard, Jossy van den; Ndaro, A.; Buretta, R.; Irongo, C.F.; Lega, D.A.; Nyombi, Balthazar; Kibiki, Gibson

doi:10.5588/ijtld.10.0762

Cited by 13 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with previous studies we found that LED-FM had a higher sensitivity, but a lower specificity compared to ZN-microscopy [11], [21]. By using the new definition of a positive smear (≥1 AFB/length) from WHO [8], LED-FM in our study showed a relatively low specificity, resulting in a significant number of false-positive results based on ‘scanty smears’.…”

Section: Discussionsupporting

confidence: 88%

Implementation of LED Fluorescence Microscopy for Diagnosis of Pulmonary and HIV-Associated Tuberculosis in a Hospital Setting in Indonesia

Chaidir

Parwati²,

Annisa³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

BackgroundFluorescence microscopy (FM) has not been implemented widely in TB endemic settings and little evaluation has been done in HIV-infected patients. We evaluated diagnostic performance, time and costs of FM with light-emitting diodes technology (LED-FM), compared with conventional (Zieh-Neelsen) microscopy in a hospital in Indonesia which acts as referral centre for HIV-infected patients.MethodWe included pulmonary tuberculosis suspects from the outpatient and HIV clinic. Direct and concentrated sputum smears were examined using LED-FM and ZN microscopy by two technicians who were blinded for the HIV-status and the result of the comparative test. Mean reading time per slide was recorded and cost of each slide was calculated. Mycobacteria culture served as the reference standard.ResultsAmong 404 tuberculosis suspects from the outpatient clinic and 256 from the HIV clinic, mycobacteria culture was positive in 12.6% and 27%, respectively. The optimal sensitivity of LED-FM was achieved by using a threshold of ≥2 AFB/length. LED-FM had a higher sensitivity (75.5% vs. 54.9%, P<0.01) but lower specificity (90.0% vs 96.6%, P<0.01) compared to ZN microscopy. HIV was associated with a lower sensitivity but similar specificity. The average reading time using LED-FM was significantly shorter (2.23±0.78 vs 5.82±1.60 minutes, P<0.01), while costs per slide were similar.ConclusionHigh sensitivity of LED-FM combined with shorter reading time of sputum smear slides make this method a potential alternative to ZN microscopy. Additional data on specificity are needed for effective implementation of this technique in high burden TB laboratories.

show abstract

Section: Discussionsupporting

confidence: 88%

Implementation of LED Fluorescence Microscopy for Diagnosis of Pulmonary and HIV-Associated Tuberculosis in a Hospital Setting in Indonesia

Chaidir

Parwati²,

Annisa³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…5 Previous studies have demonstrated that concentration of sputum by various methods signifi cantly increases the yield of PTB cases by conventional light and light-emitting diode fl uorescence microscopy (LED-FM). 6,7 Most studies used varying chemical methods and sedimentation methods, were based on a small sample, or were conducted at specialised centres. 7 While studies on optimising microscopy have been performed in reference laboratories, no studies have been reported under routine programme conditions in I ndia.…”

Section: Vol 3 No 1 Published 21 March 2013mentioning

confidence: 99%

Is bleach-sedimented smear microscopy an alternative to direct microscopy under programme conditions in India? [Short communication]

Vishnu¹,

Bhat

Bansal

et al. 2013

public health action

View full text Add to dashboard Cite

This cross-sectional multi-centric study compared the yield of and potential benefit for detecting smear-positive pulmonary tuberculosis (PTB) by bleach sedimentation (2% sodium-hypochlorite) versus direct microscopy under programme conditions in India. Among 3168 PTB suspects, 684 (21.6%) were detected by bleach sedimentation vs. 625 (19.7%) by direct microscopy, with a proportional overall agreement of 96% (κ = 0.88). While 594 patients were smear-positive with both methods, 31 patients detected by direct microscopy were missed and an additional 90 patients were detected by bleach sedimentation. Overall, bleach sedimentation increased the yield of smear-positive TB detection; however; it also increased the time to results.

show abstract

“…This helps avoid delay in diagnosis and subsequent initiation of treatment, thus preventing further spread of the disease. 8 The study intended was to determine the diag-nostic accuracy of LED-FM and conventional ZN stain-ing taking Mycobacterial tuberculosis culture as the "Gold Standard" for resource-poor settings.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Light Emitting Diode (LED) Fluorescent Microscopy with Ziehl-Neelsen Microscopy on Sputum Specimens for Diagnosis of Pulmonary Tuberculosis Keeping Culture as ‘Gold Standard’

Zahra

Saleem

Mirza

et al. 2022

PAFMJ

View full text Add to dashboard Cite

Objective: To compare LED fluorescence microscopy and Ziehl-Neelsen staining in terms of their diagnostic performance in diagnosing pulmonary tuberculosis, taking sputum specimens from patients suspected of pulmonary tuberculosis. Study Design: Prospective longitudinal study. Place and Duration of Study: Microbiology Department, Armed Forces Institute of Pathology Rawalpindi, from Jan 2019 to Dec 2019. Methodology: Sputum samples from patients with clinical suspicion of pulmonary tuberculosis were stained using Ziehl-Neelsen (ZN) stain, fluorescent stain with Auramine O staining (AO) stain and Mycobacterial culture on Mycobacterial Growth Indicator Tube (MGIT 960), to detect Mycobacterium tuberculosis. WHO guidelines were followed to grade positive smears. Results: Among 206 patients with suspicion of tuberculosis, 143 (69%) were male, and 63 (30%) were female patients. The mean age of the patients was 53.67 ± 14.73 years. Out of 206 sputum samples, 64 were negative by all three techniques used. 142 (68%) specimens detected Mycobacterium tuber-culosison MGIT960. Within 142 culture-positive samples, only 40 samples were positive on Ziehl-Neelsen microscopy, whereas 97 samples were detected positive by LED fluorescent microscopy. In culture-negative samples, three were missed on Ziehl-Neelsen staining, which was positive with Fluorescent microscopy. Sensitivity and specificity for Ziehl-Neelsen smear microscopy were 26.7% and 96.8%, respectively. Sensitivity and specificity for Fluorescent smear microscopy were 64.8% and 92.2%, respectively. Conclusion: We concluded that the efficacy of LED fluorescence microscopy has proven to have many potential advantages over conventional Ziehl-Neelsen microscopy.

show abstract

Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis

Abstract: S E T T I N G

Cited by 13 publications

References 14 publications

Implementation of LED Fluorescence Microscopy for Diagnosis of Pulmonary and HIV-Associated Tuberculosis in a Hospital Setting in Indonesia

Implementation of LED Fluorescence Microscopy for Diagnosis of Pulmonary and HIV-Associated Tuberculosis in a Hospital Setting in Indonesia

Is bleach-sedimented smear microscopy an alternative to direct microscopy under programme conditions in India? [Short communication]

Comparison of Light Emitting Diode (LED) Fluorescent Microscopy with Ziehl-Neelsen Microscopy on Sputum Specimens for Diagnosis of Pulmonary Tuberculosis Keeping Culture as ‘Gold Standard’

Contact Info

Product

Resources

About