Light dilution via wavelength management for efficient high‐density photobioreactors

Ooms, Matthew D.; Graham, Percival J.; Nguyen, Brian; Sargent, Edward H.; Sinton, David

doi:10.1002/bit.26261

Cited by 31 publications

(34 citation statements)

References 52 publications

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“…However, in terms of light quality, it has been shown that different wavelengths can enhance the sustainability of cyanobacteria cultures according to the target of production (Pagels et al, 2019). For instance, multiple studies reported that red light (650 nm) and blue light could promote the production of EPS or accelerate metabolic paths in cyanobacteria from genera Nostoc, Synechocystis and Synechococcus (Bland & Angenent, 2016;Han et al, 2017a;Han et al, 2017b;Ooms et al, 2017), and the microalgae Chlorella (Atta et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Ortiz-Moreno¹,

Cárdenas-Poblador²,

Agredo³

et al. 2020

Univ. Sci.

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Mathematical models provide information about population dynamics under different conditions. In the study, four models were evaluated and employed to describe the growth kinetics of Nostoc ellipsosporum with different light wavelengths: Baranyi-Roberts, Modiﬁed Gompertz, Modiﬁed Logistic, and Richards. N. ellipsosporum was grown in BG-11 liquid medium for 9 days, using 12 hours of photoperiod and the following treatments: white light (400-800 nm), red light (650-800 nm), yellow light (550-580 nm) and blue light (460-480 nm). Each experiment was performed in triplicate. The optical density (OD) was measured on days 1, 3, 5, 7 and 9, using a spectrophotometer at 650 nm. The maximum cell growth was obtained under white light (OD650 : 0.090 ± 0.008), followed by the yellow light (OD650 :0.057 ± 0.004). Conversely, blue light showed a marked inhibitory effect on the growth of N. ellipsosporum (OD650 : 0.009 ± 0.001). The results revealed that the Baranyi-Roberts model had a better ﬁt with the experimental data from N. ellipsosporum growth in all four treatments. The ﬁndings from this modeling study could be used in several biotechnological applications that require the productionof N. ellipsosporum and its bioproducts.

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Section: Introductionmentioning

confidence: 99%

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Ortiz-Moreno¹,

Cárdenas-Poblador²,

Agredo³

et al. 2020

Univ. Sci.

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“…A number of studies sought to dilute the supplied light intensity by optimizing the light spectra distribution. These attempts were encouraged by the integration of light emitting diodes (LEDs) within indoor PBRs ( Ooms et al, 2017 ). Tailoring light wavelength spectrum affords improved growth conditions stability and reproducibility and has been shown to lead to concrete achievements in biomass productivity and ultimately in the accumulation of useful products.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the light intensity dependence of the growth ofSynechocystisand of the light distribution in a photobioreactor energized by 635 nm light

Cordara

Pagliano

et al. 2018

PeerJ

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Synechocystis gathered momentum in modelling studies and biotechnological applications owing to multiple factors like fast growth, ability to fix carbon dioxide into valuable products, and the relative ease of genetic manipulation. Synechocystis physiology and metabolism, and consequently, the productivity of Synechocystis-based photobioreactors (PBRs), are heavily light modulated. Here, we set up a turbidostat-controlled lab-scale cultivation system in order to study the influence of varying orange–red light intensities on Synechocystis growth characteristics and photosynthetic activity. Synechocystis growth and photosynthetic activity were found to raise as supplied light intensity increased up to 500 μmol photons m−2 s−1 and to enter the photoinhibition state only at 800 μmol photons m−2 s−1. Interestingly, reverting the light to a non-photo-inhibiting intensity unveiled Synechocystis to be able to promptly recover. Furthermore, our characterization displayed a clear correlation between variations in growth rate and cell size, extending a phenomenon previously observed in other cyanobacteria. Further, we applied a modelling approach to simulate the effects produced by varying the incident light intensity on its local distribution within the PBR vessel. Our model simulations suggested that the photosynthetic activity of Synechocystis could be enhanced by finely regulating the intensity of the light incident on the PBR in order to prevent cells from experiencing light-induced stress and induce their exploitation of areas of different local light intensity formed in the vessel. In the latter case, the heterogeneous distribution of the local light intensity would allow Synechocystis for an optimized usage of light.

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“…Among hydrogels formed with 150 mM Ca 2+ , the first harvested layer achieved the greatest individual layer productivity in all cases, with the maximum of 1.0 g DW m −2 d −1 achieved in hydrogels subjected to a 3‐day harvest period. This productivity is high for S. elongatus , comparable to that of suspension cultures supplemented with elevated carbon concentrations and in similar illumination conditions (Modiri et al, ; Ooms et al, ; Silva et al, ). Overall, the biomass productivities of all harvested layers suggests a material cost of biomass production of a few tens of cents per kilograms of dry mass per year (alginate‐dominant), which is comparable with that estimated for PSBR production (Podola et al, ).…”

Section: Resultsmentioning

confidence: 63%

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Pierobon

Riordon

Nguyen

et al. 2017

Biotech & Bioengineering

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High-density biomass production is currently only realized in biofilm-based photobioreactors. Harvest yields of whole biofilms are self-limited by daughter-upon-parent cell growth that hinders light and leads to respiratory biomass losses. In this work, we demonstrate a sustainable multi-harvest approach for prolonged generation of high-density biomass. Calcium-alginate hydrogel cultures loaded with Synechococcus elongatus PCC 7942 achieved production densities comparable to that of biofilms (10 cells/mL) and optimal total productivity in harvest periods of 2 or 3 days that allowed high-density surface growth without self-limiting cell buildup or surface death. Cross-linking calcium concentration had a strong influence on surface growth and harvest yields, especially in the first harvests. Subsequent harvests achieved more uniform biomass yields and distributions, unaffected by bulk respiration or light penetration. Collectively, these results demonstrate the feasibility of sustained, high-density biomass production by periodic harvesting within microalgal hydrogel cultures. Biotechnol. Bioeng. 2017;114: 2023-2031. © 2017 Wiley Periodicals, Inc.

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Light dilution via wavelength management for efficient high‐density photobioreactors

Cited by 31 publications

References 52 publications

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Analysis of the light intensity dependence of the growth ofSynechocystisand of the light distribution in a photobioreactor energized by 635 nm light

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

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