Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Motoyama, Aracy Akiko; Watanabe, Ii-sei

doi:10.2334/josnusd.43.269

Cited by 5 publications

(7 citation statements)

References 23 publications

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“…These aspects are in accordance with the reports by MacConnachie et al (1964), and Motoyama and Watanabe (2001).…”

Section: Discussionsupporting

confidence: 80%

“…The data revealed that externally the muscular fiber is coated by collagen fibers that constitute the endomysium such as described by Morita et al (1991); Nishimura et al (1994); and Motoyama and Watanabe (2001). According to our results, the disposition of collagen fibers around each muscular fiber is characterized by fibrils similar to data showed by MacConnachie et al (1964) and Ohtani (1988).…”

Section: Discussionsupporting

confidence: 73%

“…It is accentuated that structurally the organization of the muscular layer of rat tongue has a conformation similar to those of rabbits. Some aspects of morphology of anterior third of rat tongue were reported by Motoyama and Watanabe (2001).…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Motoyama

Watanabe

Iyomasa

et al. 2009

Microscopy Res & Technique

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The nerve terminals of intrinsic muscular fibers of the tongue of adult wistar rats was studied by using silver impregnation techniques, transmission electron microscopy (TEM), and high resolution scanning electron microscopy (HRSEM) to observe the nerve fibers and their terminals. Silver impregnation was done according to Winkelman and Schmit, 1957. For TEM, small blocks were fixed in modified Karnovsky solution, postfixed in 1% buffered osmium tetroxide solution, and embedded in Spurr resin. For HRSEM, the parts were fixed in 2% osmium tetroxide solution with 1/15 M sodium phosphate buffer (pH 7.4) at 4 degrees C for 2 h, according to the technique described by Tanaka, 1989. Thick myelinated nerve bundles were histologically observed among the muscular fibers. The intrafusal nerve fiber presented a tortuous pathway with punctiform terminal axons in clusters contacting the surface of sarcolemma. Several myelinated nerve fibers involved by collagen fibers of the endoneurium were observed in HRSEM in three-dimensional aspects. The concentric lamellae of the myelin sheath and the axoplasm containing neurofilaments interspersed among the mitochondria were also noted. In TEM, myofibrils, mitochondria, rough endoplasmic reticulum, Golgi's apparatus, and glycogen granules were observed in sarcoplasm. It is also noted that the sarcomeres constituted by myofilaments with their A, I, and H bands and the electron dense Z lines. In areas adjacent to muscular fibers, there were myelinated and unmyelinated nerve fibers involved by endoneurium and perineurium. In the region of the neuromuscular junction, the contact with the sarcolemma of the muscular cell occurs forming several terminal buttons and showing numerous evaginations of the cell membrane. In the terminal button, mitochondria and numerous synaptic vesicles were observed.

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“…These aspects are in accordance with the reports by MacConnachie et al (1964), and Motoyama and Watanabe (2001).…”

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 73%

See 1 more Smart Citation

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Motoyama

Watanabe

Iyomasa

et al. 2009

Microscopy Res & Technique

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“…The technique of vascular casting followed by corrosion associated with analyses in scanning electron microscopy (SEM) described by Verli et al (2006) has been used in the study of the vasculature of the muscles in the tongue of Wistar rats (Motoyama and Watanabe 2001) and of the dorsal surface of the tongue in different animals (Kishi et al 1990;Kraether Neto 2005;Lopes et al 2002;Matsukawa and Okada 1994;Makiyama et al 1998a,b;Ojima et al 1996b;Ojima 2001;Selliseth and Selvig 1993) and humans (Yu et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Angioarchitecture of the ventral surface of the tongue from Wistar rats

et al. 2008

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The goal of this study was to describe the angioarchitecture of the ventral surface of the tongue from Wistar rats using a vascular corrosion casting technique associated with scanning electron microscopy (SEM). Six Wistar rats were used for the vascular casting method with Mercox resin. Following the resin polymerization, the tongue of each animal was dissected and corroded in a 5% sodium hydroxide solution. Once the corrosion and drying of the specimens were completed, the specimens were mounted on aluminum stubs, coated with carbon and gold and analyzed under SEM. The results showed that the ventral surface of the tongue presents simple, even and abundant vasculature constituted by a vascular plexus consisting of a superficial vascular network and by the ranine veins. The superficial vascular network, made up of the ascending and descending branches, presents as a loose network, with little morphological variation between the capillary loops.

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“…1991, Ojima et al . 1997, in a 5% formic acid solution for 5 or 30 min (Selliseth and Selvig 1993, Kraether Neto 2005, Verli 2006), in collagenaseassociated hydrochloric acid (HCl) solution (Motoyama and Watanabe 2001) and at increasing concentrations of alcohol Selvig 1995, Pithan 2005).…”

Section: Dissectionmentioning

confidence: 99%

Vascular Corrosion Casting Technique Steps

et al. 2007

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The vascular corrosion casting technique produces a replica of vascular beds of normal or pathological tissues. Once associated with scanning electron microscopy (SEM), this technique provides details of the three-dimensional anatomic arrangement of the vascular replica, which is the main advantage of this method. The present study is intended to describe the steps of the vascular corrosion casting technique and the different ways to perform them. them.

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Light and scanning electron microscopic studies of the angioarchitecture of intrinsic muscle fibers of the anterior rat tongue.

Cited by 5 publications

References 23 publications

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Angioarchitecture of the ventral surface of the tongue from Wistar rats

Vascular Corrosion Casting Technique Steps

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