Angioarchitecture of the ventral surface of the tongue from Wistar rats

Verli, Flaviana Dornela; Marinho, Sandra Aparecida; Rossi-Schneider, Tíssiana Rachel; Yurgel, Liliane Soares; Souza, Maria Antonieta Lopes de

doi:10.1002/sca.20122

Cited by 11 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scanning electron microscopy was used to evaluate cardiac microvascular integrity (28). In control animals, the surface of cardiac microvessels was smooth and well-integrated (Fig.…”

Section: Resultsmentioning

confidence: 99%

Glucagon-Like Peptide-1 Protects Against Cardiac Microvascular Injury in Diabetes via a cAMP/PKA/Rho-Dependent Mechanism

et al. 2013

View full text Add to dashboard Cite

Impaired cardiac microvascular function contributes to cardiovascular complications in diabetes. Glucagon-like peptide-1 (GLP-1) exhibits potential cardioprotective properties in addition to its glucose-lowering effect. This study was designed to evaluate the impact of GLP-1 on cardiac microvascular injury in diabetes and the underlying mechanism involved. Experimental diabetes was induced using streptozotocin in rats. Cohorts of diabetic rats received a 12-week treatment of vildagliptin (dipeptidyl peptidase-4 inhibitor) or exenatide (GLP-1 analog). Experimental diabetes attenuated cardiac function, glucose uptake, and microvascular barrier function, which were significantly improved by vildagliptin or exenatide treatment. Cardiac microvascular endothelial cells (CMECs) were isolated and cultured in normal or high glucose medium with or without GLP-1. GLP-1 decreased high-glucose–induced reactive oxygen species production and apoptotic index, as well as the levels of NADPH oxidase such as p47phox and gp91phox. Furthermore, cAMP/PKA (cAMP-dependent protein kinase activity) was increased and Rho-expression was decreased in high-glucose–induced CMECs after GLP-1 treatment. In conclusion, GLP-1 could protect the cardiac microvessels against oxidative stress, apoptosis, and the resultant microvascular barrier dysfunction in diabetes, which may contribute to the improvement of cardiac function and cardiac glucose metabolism in diabetes. The protective effects of GLP-1 are dependent on downstream inhibition of Rho through a cAMP/PKA-mediated pathway.

show abstract

“…Scanning electron microscopy was used to evaluate cardiac microvascular integrity (28). In control animals, the surface of cardiac microvessels was smooth and well-integrated (Fig.…”

Section: Resultsmentioning

confidence: 99%

Glucagon-Like Peptide-1 Protects Against Cardiac Microvascular Injury in Diabetes via a cAMP/PKA/Rho-Dependent Mechanism

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The tongues of the WWCPS rats had a macroscopic structure comparable to the tongues of other rat lines (Al-Refai et al 2014), the Wistar rat (Kullaa-Mikkonen et al 1987, Picoli et al 2006Verli et al 2008;Costa et al 2013) or the Sprague-Dawley rat (Costa et al 2013;Reginato et al 2014). The median groove in the WWCPS was approximately 1 cm long and was found only in the apex.…”

Section: Discussionmentioning

confidence: 66%

“…The structure of the rat tongue in different strains and breeds of rats has been described previously using light microscopy and ultrastructural studies (Hosley and Oakley 1987;Iwasaki et al 1987Iwasaki et al , 1997Iwasaki et al , 1999Kullaa-Mikkonen et al 1987;Nagato et al 1997;Iino and Kobayashi 1988;Jakob et al 1998;Wakisaka et al 1998;Triantafyllou et al 2002;Yücel et al 2002;de Abreu et al 2006;Picoli et al 2006;Verli et al 2008;Abayomi et al 2009;Lopes et al 2009;Costa et al 2013;Al-Refai et al 2014;El Sharaby et al 2014;Reginato et al 2014). However, the majority of those studies did not assess gender differences, and differences between wild and laboratory rats.…”

Section: Introductionmentioning

confidence: 99%

Subject Index to Volume 81

2006

Anat Sci Int

View full text Add to dashboard Cite

The aim of this study was to characterise the tongue in wild-type rats using several microscopic techniques. Warsaw Wild Captive Pisula Stryjek (WWCPS) rats belong to a lineage of wild-caught rats. The study was carried out on tongues of 15 male and 15 female WWCPS rats. Histological, histochemical and ultrastructural studies were carried out. There were no significant differences between the male and female WWCPS rat tongues. There was a median groove approximately 1 cm long in the apex of the tongue that faded caudally. The intermolar prominence was clearly marked in the distal part of the lingual body. Lingual mechanical papillae located on the surface of the tongue formed four subtypes based on their shape: small filiform papillae, giant filiform papillae, thin elongated filiform papillae and wide filiform papillae. Gustatory papillae formed the second group of papillae and were divided into bud-shaped fungiform papillae, a single vallate papilla surrounded by an incomplete papillary groove and foliate papillae, which were a well-formed and composed of several pairs of folds divided by longitudinal grooves. In the posterior lingual glands (mucoserous and serous), acidic sulphated mucin-secreting cells gave a strong AB pH 2.5 positive reaction, and a positive reaction with the AB pH 1.0 stain for acidic carboxylated mucin. Double AB/PAS staining showed the presence of the majority of mucous cells with predominant of acidic mucins. Positive PAS staining showed the presence of neutral mucin. HDI staining demonstrated a weak positive reaction within Weber's glands of the WWCPS rat tongue.

show abstract

“…Mercox (Company, Location) is a methylmethacrylate-based resin widely used for vascular corrosion casting with subsequent scanning electron microscopy[11], [26]. Rat hearts were removed and injected with 10 ml Mercox resin (SPI) (100 mmHg) through the aorta according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

FTY720 Protects Cardiac Microvessels of Diabetes: A Critical Role of S1P1/3 in Diabetic Heart Disease

et al. 2012

View full text Add to dashboard Cite

Background: Diabetes is associated with an increased risk of cardiac microvascular disease. The mechanisms by which this damage occurs are unknown. However, research suggests that signaling through the sphingosine-1-phosphates receptor 1 and 3 (S1P1/3) by FTY720, a sphiongolipid drug that is structually similar to SIP, may play a role in the treatment on cardiac microvascular dysfunction in diabetes. We hypothesized that FTY720 might exert the cardioprotective effects of S1P1 and S1P3 viaprotein kinase C-beta (PKCβ II) signaling pathway. Methodology/Principal Findings: Transthoracic echocardiography was performed to detect the change of cardiac function. Scanning and transmission electron microscope with lanthanum tracer were used to determine microvascular ultrastructure and permeability in vivo. Apoptosis was detected by TUNEL and CD31 dual labeling in paraffin-embedded sections. Laser capture miscrodissection was used to assess cardiac micovascular endothelial cells (CMECs) in vivo. RT-PCR and Western blot analysis were used to determine the mRNA levels and protein expression of S1P1, S1P3, and PKCβ II. In the diabetic rats vs. controls, cardiac capillaries showed significantly higher density; CD31 positive endothelial cells were significantly reduced; the apoptosis index of cardiac endothlial cells was significantly higher. And FTY720 could increase the expressional level of S1P1 and boost S1P3 trasnslocation from membrane to nuclear, then ameliorate cardiac microvascular barrier impairment and pathologic angiogenesis induced by diabetes. In addition, overexpression of PKCβ II significantly decreased the protective effect of FTY720. Conclusions: Our study represents that the deregulation of S1P1 and S1P3 is an important signalresponsible for cardiac microvascular dysfunction in diabetes. FTY720 might be competent to serve as a potential therapeutic approach for diabetic heart disease through ameliorating cardiac microvascular barrier impairment and pathologic angiogenesis, which might be partly dependent on PKCβII-mediated signaling pathway.

show abstract

Angioarchitecture of the ventral surface of the tongue from Wistar rats

Cited by 11 publications

References 16 publications

Glucagon-Like Peptide-1 Protects Against Cardiac Microvascular Injury in Diabetes via a cAMP/PKA/Rho-Dependent Mechanism

Glucagon-Like Peptide-1 Protects Against Cardiac Microvascular Injury in Diabetes via a cAMP/PKA/Rho-Dependent Mechanism

Subject Index to Volume 81

FTY720 Protects Cardiac Microvessels of Diabetes: A Critical Role of S1P1/3 in Diabetic Heart Disease

Contact Info

Product

Resources

About