Light and Electron Microscopy Studies of the Infection of the Western Flower ThripsFrankliniella occidentalis(Thysanoptera: Thripidae) by the Entomopathogenic FungusMetarhizium anisopliae

Vestergaard, Susanne; Butt, Tariq M.; Bresciani, J.; Gillespie, A. T.; Eilenberg, Jørgen

doi:10.1006/jipa.1998.4802

Cited by 75 publications

(79 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…'Late-removed' larvae, on the other hand, contained mycelial growth inside their bodies ( figure 3c(ii)). This confirms the time pattern of Metarhizium infection in other arthropods, where attachment of spores to the host cuticle and its penetration was found to occur after approximately 6 h and 24 -48 h, respectively (Vestergaard et al 1999;Arruda et al 2005). Thus, ants from both colony types reacted to signs of larval sickness by late larval removal, but only ants from diverse colonies had the additional capacity to perceive and/or react to the presence of the live, pathogenic fungal spores prior to infection.…”

Section: Discussionsupporting

confidence: 80%

Rapid anti-pathogen response in ant societies relies on high genetic diversity

et al. 2010

View full text Add to dashboard Cite

Social organisms are constantly exposed to infectious agents via physical contact with conspecifics. While previous work has shown that disease susceptibility at the individual and group level is influenced by genetic diversity within and between group members, it remains poorly understood how group-level resistance to pathogens relates directly to individual physiology, defence behaviour and social interactions. We investigated the effects of high versus low genetic diversity on both the individual and collective disease defences in the ant Cardiocondyla obscurior. We compared the antiseptic behaviours (grooming and hygienic behaviour) of workers from genetically homogeneous and diverse colonies after exposure of their brood to the entomopathogenic fungus Metarhizium anisopliae. While workers from diverse colonies performed intensive allogrooming and quickly removed larvae covered with live fungal spores from the nest, workers from homogeneous colonies only removed sick larvae late after infection. This difference was not caused by a reduced repertoire of antiseptic behaviours or a generally decreased brood care activity in ants from homogeneous colonies. Our data instead suggest that reduced genetic diversity compromises the ability of Cardiocondyla colonies to quickly detect or react to the presence of pathogenic fungal spores before an infection is established, thereby affecting the dynamics of social immunity in the colony.

show abstract

Section: Discussionsupporting

confidence: 80%

Rapid anti-pathogen response in ant societies relies on high genetic diversity

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Scanning electron microscopic (SEM) study of cotton leaf with mummified whiteflies: Scanning EM has frequently been used to study the mode of pathogenesis in host pest-pathogen interactions. These studies have helped in determining the virulence attributes of fungal strains and in identification of insect barriers to infection (Vestergaard et al 1999). The fine morphology of P. oxalicum spg1 hyphae as observed in Fig.10 showed formation of dense network of mycelium on the surface of cotton leaf.…”

Section: Fig 15 Scanning Electron Micrograph Of Dead and Decayed Camentioning

confidence: 99%

Penicillum oxalicum spg1: A novel entomopathogenic fungus isolated from mummified Bemisia tabaci (Gennadius) of cotton

Kataria

Singh

Pandove

et al. 2018

JANS

View full text Add to dashboard Cite

Whitefly has assumed the status of a serious pest of cotton in north India in recent past due to its severe attack on cotton crop. The productivity of cotton crop has fallen substantially from 574 kg ha -1 of lint in the year 2014 -15 to 197 kg ha -1 in the year 2015-16 in Punjab. During extensive epidemiological surveillance of whitefly on cotton crop in the month of September 2016, mummified whiteflies with fungus were noticed on both abaxial and adaxial surfaces of leaves of the infested cotton plant at village, Mandi Khurd, Mansa District of Punjab, India and at Punjab Agricultural University, Regional Research Station, Bathinda, India. The fungus was isolated and purified from the mummified white fly and characterized at molecular level by sequencing 633 bp D2 region of Large Subunit (LSU) rRNA gene and identified as Penicillium oxalicum spg1. The gene sequence has been submitted to NCBI, USA with accession no. KY214238. The microscopic studies (stereo-and scanning electron) of the cotton leaves with mummified whitefly further strengthen the entomopathogenic potential of P. oxalicum spg1. There are few reports of entomopathogenic potential of P. oxalicum spg1 against other insects and pests. To our knowledge, this is the first report of isolation and identification of P. oxalicum spg1 from mummified white fly. It's potential as a biocontrol agent against white fly can be exploited in a promising way with nominal interference with biological equilibrium.

show abstract

“…3A). Este resultado difere do obtido por Vestergaard et al (1999) que, ao estudarem a distribuição dos conídios no corpo de larvas de Frankliniella occidentalis infectadas por M. anisopliae, encontraram preferência por sítios específicos. Ademais, relataram germinação de conídios próxima da totalidade no prazo de um dia após a infecção.…”

Section: Figura 2 Larvas De Rhipicephalus Sanguineus Após a Infecçãounclassified

Eventos externos e internos da infecção de larvas e ninfas de Rhipicephalus sanguineus por Metarhizium anisopliae

Garcia

Monteiro

Szabó

et al. 2008

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

RESUMOExaminaram-se a adesão, a germinação, a penetração e a colonização de larvas e ninfas de Rhipicephalus sanguineus por Metarhizium anisopliae, assim como as lesões infringidas pelo fungo nas respectivas fases do ciclo de vida do ácaro. Realizaram-se infecções experimentais em 11 grupos contendo 250 larvas e 11 grupos contendo 75 ninfas de R. sanguineus, por meio de banho, durante três minutos sob agitação manual, em suspensão contendo 10 8 conídios/ml do fungo. Nos grupos-controles, o banho foi realizado usando o veículo da suspensão. Larvas e ninfas foram processadas para um estudo histopatológico e de microscopia eletrônica de varredura nos seguintes tempos após a infecção: uma e 18 horas, e um, dois, três, quatro, cinco, seis, sete, nove e 11 dias. A germinação dos conídios ocorreu em até 18 horas pós-inoculação, e o fungo penetrou nas larvas e ninfas através do tegumento, dois e três dias após a infecção, respectivamente. Após penetração, o fungo invadiu o corpo das larvas e ninfas, promovendo uma colonização difusa, sem preferência aparente por tecidos específicos. Lesões significativas não foram observadas. A morte das larvas e ninfas ocorreu no terceiro e quarto dias pós-infecção, e a esporulação do patógeno sobre o cadáver foi iniciada no sexto dia pós-infecção.Palavras-chave: cão, carrapato, controle biológico, controle microbiano, fungo entomopatogênico ABSTRACT The adhesion, germination and colonization of Rhipicephalus sanguineus larvae and nymphs by Metarhizium anisopliae as well as the lesions caused by the fungus were studied. For this purpose, 11 groups of 250 larvae each and 11 groups of 75 nymphs each were bathed during 3 minutes under manual shaking in a 108 conidia/ml suspension. Corresponding control groups were bathed only in the suspension vehicle. Ticks were also submitted to both conventional microscopy and scanning eletronmicrocopy analyses at several post-infection periods (1 and 18 hours and 1, 2, 3, 4, 5, 6, 7, 9, and 11 days)

show abstract

Light and Electron Microscopy Studies of the Infection of the Western Flower ThripsFrankliniella occidentalis(Thysanoptera: Thripidae) by the Entomopathogenic FungusMetarhizium anisopliae

Cited by 75 publications

References 19 publications

Rapid anti-pathogen response in ant societies relies on high genetic diversity

Rapid anti-pathogen response in ant societies relies on high genetic diversity

Penicillum oxalicum spg1: A novel entomopathogenic fungus isolated from mummified Bemisia tabaci (Gennadius) of cotton

Eventos externos e internos da infecção de larvas e ninfas de Rhipicephalus sanguineus por Metarhizium anisopliae

Contact Info

Product

Resources

About