Light and electron microscopy of the leucocytes of Crassostrea virginica (Mollusca: Pelecypoda)

Feng, S. Y.; Feng, J. S.; Burke, C. N.; Khairallah, L. H.

doi:10.1007/bf00335537

Cited by 84 publications

(29 citation statements)

References 33 publications

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“…In general, two distinct classes of cells exist and they are granular and agranular hemocytes. In the case of Crassostrea virginica and Mercenaria campechiensis, the presence of agranular hemocytes and granular hemocytes with refractile granules reported in this study are in agreement with those of Feng et al, (1971) and .…”

Section: Discussionsupporting

confidence: 89%

“…Several attempts have been made to describe the hemolymph cells of various marine shellfish (Takatsuk, 1934;Galtsoff, 1964;Feng et al, 1971;Ruddell, 1971; and to study their phagocytic activity towards various bacteria Cheng & Cali, 1974;Cheng, 1975;Hardy et al, 1977). In general, two distinct classes of cells exist and they are granular and agranular hemocytes.…”

Section: Discussionmentioning

confidence: 99%

“…C o 11 e c t i o n o f h e m o 1 y m p h. In all three molluscs, hemolymph samples were collected from the anterior adductor muscle sinus by using endotoxin free hypodermic needles and syringes (Feng et al, 1971). Preparation of hemolymph.…”

Section: Methodsmentioning

confidence: 99%

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Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria

Rodrick

Ulrich

1984

Helgolander Meeresunters

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Hemocytes represent one of the most important defense mechanisms against foreign material in Mollusca. The morphology, hematological parameters and behaviour of hemolymph cells were studied in the southern quahog Mercenaria campechiensis, the eastern oyster Crassostrea virginica, and the blood ark Anadara ovalis challenged with the bacteria Vibrio vulnificus and V. anguillarum. Two general classes of hemocytes (granular and agranular) exist in C. virginica and M. campechiensis. In contrast, A. ovalis possesses 3 general classes (granular, agranular and erythrocytes). Three types of granules were identified by light microscopy. When hemolymph cells were studied by transmission electron microscopy, the cytoplasm of hemolymph cells was noted to contain many organelles, including electron dense granules. Both agranular and granular hemolymph cells were capable of colchicine-sensitive pseudopodial movement and spreading. The results indicate that marine bivalves possess hemolymph blood cells which may play a role in the internal defense paralleling mammalian phagocytes. The morphology of these cells, as determined by light, scanning and transmission electron microscopy, showed some similarity to mammalianmononuclear phagocytes. The sub-cellular events of molluscan hemocyte phagocytosis of V.vulnificus and V~ anguillarum were studied by both scanning and transmission electron microscopy. The role of these cells and the factors which govern their behavior are of economic and public health importance.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria

Rodrick

Ulrich

1984

Helgolander Meeresunters

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“…The increase of cytoplasmic inclusions of the granulocytes in the present results may be a sign of lysosomal activity of the cell. Feng et al (1971) reported that, the electron -dense granules of the granulocytes could be considered as lysosomes with hydrolase enzymes concerning with intracellular digestion of phagocytic materials in the cells of Crassostrea verginica. The formation of aggregates of haemocytes may be due to the attraction and arrangment of these cells around the extracellular substances which were derived from toxic materials, as described by Abdul-Salam and Michelson (1980) during larval trematode infection.…”

Section: Discussionmentioning

confidence: 99%

Effect of Euphorbia Peplus Plant Extract and the Antihelmenthic Prazequantel on the Defence System of Biomphalaria Alexandria Snail

Helal

ElMehlawy

Rizk

et al. 2003

Egypt. J. of Aquatic Biolo. and Fish.

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T he effect of sublethal concentrations of£ pephis water suspension and praziquantel water solution on the defense system of Biomphalaria cdexandrina was examined by laboratory experiments.The mean number of haemocytes decreased insignificantly after the first week of snail exposure to the plant extract-Thereafter, a significant increase occurred after the second week of exposure. Treatment with praziquantel showed a significant increase in haemocytes after the 3 rd and 4 lh weeks of exposure. Haemocytosis represents a response to external stress or certain stimuli. The haemocytes showed behavioral as well as structural changes after long exposure to the tested mollusclcides. The cells had many cytoplasmic inclusions and produce many long spike-like pseudopodia. Haemocytes exhibited an enhanced tendency to aggregate in clusters. Such alterations may be indicative of a state of haemocytes activation. The total plasma protein concentration after treatment with E. pephis and Praziquantel increased plasma than in control snails while no changes in protein concentration of the digestive gland extract. This may be a result of an imbalance in the rate of protein synthesis and the rate of its degradation.The treatment with E peplus and Praziquantel caused a marked increase in acid phosphatase after the 2nd and 4 lh weeks. On the other hand, alkaline phosphatase activity increased after the 4 th week. This could be explained as defensive and lysosomal activity of the haemocytes. After treatment with the tested substances the plasma and digestive gland extract were capable to agglutinate sheep red 502

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“…Nakahara & Bevelander (1969) described lymphocytes and granulocytes in Pinctada radiata. Agranular and granular leukocytes have been reported in Crassostrea virginica (Cheng & Rifkin, 1970;Feng et al, 1971) and Tridacna maxima (Reade & Reade, 1972). Foley & Cheng (1974) observed hyalinocytes and fibrocytes (both agranular) and granulocytes in Mercenaria mercenaria, and Moore & Lowe (1977) classified the haemocytes of Mytilus edulis as lymphocytes (agranular), macrophages and eosinophilic granulocytes.…”

Section: Introductionmentioning

confidence: 92%

Haemocytes of the clam Tapes philippinarum (Adams & Reeve, 1850): morphofunctional characterisation

Cima

Matozzo

Marin

et al. 2000

Fish & Shellfish Immunology

134

117

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Light and electron microscopy of the leucocytes of Crassostrea virginica (Mollusca: Pelecypoda)

Cited by 84 publications

References 33 publications

Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria

Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria

Effect of Euphorbia Peplus Plant Extract and the Antihelmenthic Prazequantel on the Defence System of Biomphalaria Alexandria Snail

Haemocytes of the clam Tapes philippinarum (Adams & Reeve, 1850): morphofunctional characterisation

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