Light and Electron Microscopical Localization of Lysosomal Acid Hydrolases in Bivalve Haemocytes by Enzyme Cytochemistry.

Cajaraville, Miren P.; Pal, Santi G.; Robledo, Yolanda

doi:10.1267/ahc.28.409

Cited by 37 publications

(12 citation statements)

References 29 publications

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“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which β-glucuronidase 2.8 ± 1.2 3.6 ± 0.5 9.5 ± 1.8*** 5.8 ± 0.6*** Acid phosphatase 12.3 ± 1.0 25.6 ± 0.6 13.1 ± 2.1 ns 23.9 ± 3.2 ns Table 3. Chamelea gallina.…”

Section: Discussionsupporting

confidence: 66%

“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which (Cajaraville & Pal 1995. Chamelea gallina haemocytes display a good adhering ability, unlike the results reported for other venerid species (Foley & Cheng 1974.…”

Section: Discussionmentioning

confidence: 52%

“…The presence of binucleated circulating cells (both granulocytes and hyalinocytes) suggests that differentiated haemocytes may retain the ability to divide, and supports the hypothesis that granulocytes and hyalinocytes represent 2 different cell lines, in agreement with the results reported by Moore & Lowe (1977) for Mytilus edulis and by Carballal et al (1997b) for M. galloprovincialis, although hyalinocytes are considered by some authors (Ottaviani et al 1998) as a young differentiation stage of haemocytes, leading to granulocytes. Moreover, other studies have confirmed various stages of granulocyte differentiation with parallel growth of their specific granules (Cajaraville & Pal 1995. Chamelea gallina haemocytes display a good adhering ability, unlike the results reported for other venerid species (Foley & Cheng 1974.…”

Section: Discussionmentioning

confidence: 89%

“…Acid phosphatase is a typical marker for lysosomes (de Duve 1963), even though extralysosomal localisations of these enzymes have been described in several kinds of cells (Borgers & Verheyan 1985). Particularly in bivalves, a high activity of acid phosphatase was found in Ruditapes decussatus (Bayne et al 1979, Cajaraville et al 1995, and M. edulis (Moore & Lowe 1977). Its presence was not reported in T. philippinarum (Cima et al 2002).…”

Section: Discussionmentioning

confidence: 94%

“…Non-specific esterase activity was found in Ostrea edulis and Crassostrea gigas haemocytes by Chagot (1989), in Mya arenaria by Huffman & Tripp (1982), in Tapes philippinarum by Cima et al (2002), in M. galloprovincialis by Carballal et al (1997a). Mollusc arylsulphatase has been located in the lysosomes, and less consistently in the Golgi bodies and endoplasmic reticulum, of many different tissues (Pipe & Moore 1985, Pipe 1987, Cajaraville et al 1995, Pipe et al 1997. Unlike esterases, β-glucuronidase activity was infrequent in Chamelea gallina haemocytes, in agreement with results reported for other bivalves (Bayne et al 1979, Pipe 1990, Ottaviani et al 1998.…”

Section: Discussionmentioning

confidence: 99%

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Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Pampanin

Marin²,

Ballarin³

2002

Dis. Aquat. Org.

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A morphological and enzymatic characterization of Chamelea gallina haemocytes was carried out as a prerequisite for further studies on venus clam immunobiology. Two main types of circulating haemocytes were identified (1) hyalinocytes (79.2%), agranular cells with a central nucleus surrounded by a little cytoplasm, and (2) granulocytes (16.5%), smaller granular cells with smaller nuclei. Small cells with a strongly basophilic nucleus and a thin layer of peripheral cytoplasm, probably undifferentiated blast cells (4.3%), were also observed. Both granulocytes and hyalinocytes can assume a spreading or round morphology. The enzymatic activities of haemocytes were also investigated. Some of the granulocytes and hyalinocytes were positive for hydrolytic enzymes, suggesting a role for these cells in phagocytosis; no oxidative enzymes were detected in C. gallina haemocytes. Granulocytes and hyalinocytes can easily adhere to the substratum and exhibit a low phagocytosis activity towards foreign particles (6.3%), whereas the fraction of cells containing ingested material significantly increased after pre-incubation of test particles with cell-free haemolymph, which suggests the presence of opsonin(s) in the haemolymph. KEY WORDS: Haemocytes · Chamelea gallina · Cytoenzymology · Phagocytosis Resale or republication not permitted without written consent of the publisherDis Aquat Org 49: [227][228][229][230][231][232][233][234] 2002 (Cheney 1971, Foley & Cheng 1974, Moore & Eble 1977, Huffman & Tripp 1982. Since no data are available concerning Chamelea gallina haemocytes and their role in immunity, we carried out a morpho-functional characterization of C. gallina haemocytes as a prerequisite for further studies on the immunobiology of the venus clam in order to define a series of immuno-biomarkers to be used in the evaluation of stress conditions for this species.Our results indicate the presence of 2 main haemocyte types, namely granulocytes and hyalinocytes, both exhibiting lysosomal enzymes and phagocytosis activity. MATERIALS AND METHODSClams (shell length 20 to 30 mm) were collected with a hydraulic dredge at Venice Lido, on the west coast of the Northern Adriatic Sea, in front of the Venice lagoon. They were kept in the laboratory for 2 to 3 d before experiments in 70 l tanks filled with aerated seawater that was renewed every other day (salinity 35 ± 1 ‰; temperature 17 ± 1°C) and fed with microalgae (Isochrysis galbana and Phaeodactylum tricornutum).Heamolymph collection. Haemolymph was collected from the anterior adductor muscle of clams with a syringe containing 10 mM of L-cysteine in filtered seawater (FSW), pH 7.5, to a final dilution of 1:1 haemolymph/L-cysteine. Haemolymph was also collected in the absence of L-cysteine and centrifuged at 780 × g for 10 min to pellet cells and obtain cell-free haemolymph (CFH).Haemocyte cultures. Haemolymph was centrifuged at 780 × g for 10 min and the pellet was resuspended in FSW to achieve a final concentration of 10 6 cells ml -1 . Sixty µl of haemocyte susp...

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Section: Discussionsupporting

confidence: 66%

“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which (Cajaraville & Pal 1995. Chamelea gallina haemocytes display a good adhering ability, unlike the results reported for other venerid species (Foley & Cheng 1974.…”

Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Pampanin

Marin²,

Ballarin³

2002

Dis. Aquat. Org.

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A comparative account of coelomocyte of earthworm ecotypes with reference to its morphology, morphometry, density, phagocytosis, autofluorescence, and oxidative status

Manna

Ray

Gautam

et al. 2022

Journal of Morphology

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Earthworms inhabit different strata of moist soil. Epigeic and endogeic earthworms prefer superficial and inner stratum of soil respectively, whereas, semiaquatic species are distributed around hydrated soil near ponds and lakes. Coelomocytes, the chief immunoeffector cells of coelomic origin, perform diverse physiological functions like phagocytosis, maintenance of cellular homeostasis, and acid–base balance of coelomic fluid, graft rejection, elicitation of cytotoxic, and oxidative responses under the challenges of pathogens and toxins. The present study aims to analyze selected morphological and functional parameters in three differentially adapted Indian earthworms of nonsimilar habitats. Coelomocytes of Glyphidrilus tuberosus (Stephenson, 1916) (semiaquatic), Perionyx excavatus (Perrier, 1872) (epigeic), and Eutyphoeus orientalis (Beddard, 1883) (endogeic) were isolated for morphological and morphometric analyses and subjected to determination of phagocytic, oxidative, and cytotoxic responses. Activities of phenoloxidase, pro, and antioxidant enzymes, and autofluorescence were determined in the extruded coelomocytes of earthworms of three contrasting habitats. The differential result may be correlated with species‐specific responses and variation in habitat preference and related adaptation.

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In VitroActivities in Mussel Hemocytes as Biomarkers of Environmental Quality: A Case Study in the Abra Estuary (Biscay Bay)

Cajaraville

Olabarrieta

Marigómez

1996

Ecotoxicology and Environmental Safety

116

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Light and Electron Microscopical Localization of Lysosomal Acid Hydrolases in Bivalve Haemocytes by Enzyme Cytochemistry.

Cited by 37 publications

References 29 publications

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

A comparative account of coelomocyte of earthworm ecotypes with reference to its morphology, morphometry, density, phagocytosis, autofluorescence, and oxidative status

In VitroActivities in Mussel Hemocytes as Biomarkers of Environmental Quality: A Case Study in the Abra Estuary (Biscay Bay)

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