Light and electron microscopic study of Pelomyxa flava sp.n. (archamoebae, pelobiontida)

Pelomyxa doughnuta sp. nov. (Archamoebea, Pelobiontida) with an unusual nucleus‐glycogen association

“…In addition, in one of the electron micrographs, we managed to find a cross-section through a band of microtubules, lying in the hyaloplasm near the cell membrane. The arrangement of microtubules in this section is very similar to that in the lateral rootlet derivative of kinetosomes in P. prima, P. flava, and P. paradoxa (Chistyakova et al, 2014;Frolov, Chistyakova, et al, 2005;Frolov, Chystyakova, et al, 2011). According to modern views, kinetosomes of pelomyxae may be associated with some elements of the flagellar rootlet system such as radial microtubules, basal microtubules, and the lateral microtubular rootlet, while the overall organization of their rootlet system is species-specific (Chistyakova, Berdieva, et al, 2020;Chistyakova et al, 2013;Frolov, 2011;Walker et al, 2017).…”

“…flava , and P . paradoxa (Chistyakova et al, 2014; Frolov, Chistyakova, et al, 2005; Frolov, Chystyakova, et al, 2011). According to modern views, kinetosomes of pelomyxae may be associated with some elements of the flagellar rootlet system such as radial microtubules, basal microtubules, and the lateral microtubular rootlet, while the overall organization of their rootlet system is species‐specific (Chistyakova, Berdieva, et al, 2020; Chistyakova et al, 2013; Frolov, 2011; Walker et al, 2017).…”

Early evolution of eukaryote feeding modes, cell structural diversity, and classification of the protozoan phyla Loukozoa, Sulcozoa, and Choanozoa

“…I expressed scepticism earlier about that merger (Cavalier-Smith 1991b) and here reverse it because the nucleation point and structure of its rhizostyle (Brugerolle 1982) differ from that of the lateral root of all other Archamoebae now studied. Mastigamoeba and Mastigella Walker et al 2001) and two species of Pelomyxa (prima and flava: Frolov et al 2005Frolov et al , 2011 have a lateral microtubular root of a single broad band of microtubules nucleated by a dense sheet attached near the upper end of the centriole. This root probably evolved from the anterior dorsal root of Sulcozoa by adding more microtubules whilst retaining its original nucleation position.…”

First multigene analysis of Archamoebae (Amoebozoa: Conosa) robustly reveals its phylogeny and shows that Entamoebidae represents a deep lineage of the group

“…This interpretation is, however, problematic in two main aspects: (1) the superficial microtubules of other Conosa and eukaryotes form a sheet anchoring close to the dorsal side of the anterior basal body, so MTA-2 would have had to undergo intricate rearrangements during the evolution of Archamoebae. 2Radiating microtubules arise in multiple layers from the basal body of several Archamoebae (see Brugerolle 1982Brugerolle , 1991Simpson et al 1997;Walker et al, 2001;Frolov et al 2011). Such architecture is highly unusual for superficial microtubules since they form a monolayer in other eukaryotes.…”