Light and Electron Microscopic Study on the Possible Protective Effect of Nigella Sativa Oil on Cisplatin Hepatotoxicity in Albino Rats

Salem, Maysara M.; Altayeb, Zainab M.

doi:10.21608/ejh.2017.3698

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…These results agreed with those of Barakat et al [45] , who reported that intake of of NsL.oil decreased the serum levels of liver and kidney injury markers. Additionally, Salem, and Altayeb, (2017) [13] reported ameliorated effect of Ns oil on degenerative changes induced in liver by cisplatin treatment, when it administrated in combination with cisplatin treatment, as there was significant improvement in the biochemical alterations regarding ALT that is more specific for liver. Same findings were detected by Gani and John [46] as they suggested the possibility of Ns oil to protect hepatocytes and so decrease the leakage of liver enzymes.…”

Section: Discussionmentioning

confidence: 98%

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Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Mohammed

2020

Journal of Medical Histology

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Introduction: Ceftriaxone is a broad-spectrum cephalosporin with a hepatotoxic effect. Nigella sativa (Ns) has potent antioxidant effects, so it has a promising medicinal application. Aim: To study the possible protective role of Nigella Sativa Extracted Oil against the Hepato-toxic Effect of Ceftriaxone in adult male albino rat. Materials and Methods: Thirty two adult male albino rats had been divided into four groups with 8 rats allocated in each group: Group 1 (control group), Group 2 (Ns oil administrated): received Nigella sativa (NS) oil 2 ml/kg by gastric tube daily for 4 weeks, Group 3 (ceftriaxone treated): received ceftriaxone (360 mg/kg) (I.P) daily for 4 weeks and Group 4 (Ns oil and ceftriaxone treated group). At the last day of the experiment, the liver specimens of each animal were dissected carefully and were processed for light-microscopic examination. Results: Ceftriaxone treated group revealed elevated level of liver enzymes that were relatively lower in ceftriaxone and Ns oil group. Histological examination of the liver of ceftriaxone treated group showed dilated congested central vein and sinusoids with hydropic degeneration of hepatocytes, while portal triad revealed dilated congested portal vein and dilated bile ducts surrounded by inflammatory cells. Additionally, abundant deposition of collagen was detected and increased expression of TNFα immune staining within hepatocytes and in lining of sinusoids. All these histological alterations were less marked in group (4) which received NS oil in combination with ceftriaxone. Conclusion: Ns oil has a protective effect against liver injury resulted from ceftriaxone injection due to its antioxidant activities.

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Section: Discussionmentioning

confidence: 98%

“…Group 2 (Ns oil administrated): included 8 animals, each animal received NsL oil 2 ml/kg BW by gastric tube daily for 4 weeks. The animals were fasted 2 hours before NsL oil administration [13] .…”

Section: Experimental Designmentioning

confidence: 99%

Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Mohammed

2020

Journal of Medical Histology

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“…This was double stained with lead and uranyl acetate. The sections were examined by a JEOL-JEM-100 SX electron microscope at 80 kilo vol (Jeol Ltd., Tokyo, Japan) at the electron microscope unit in the Faculty of Agriculture, Mansoura University, Mansoura, Egypt [ 101 ].…”

Section: Methodsmentioning

confidence: 99%

Activation of SIRT-1 Pathway by Nanoceria Sheds Light on Its Ameliorative Effect on Doxorubicin-Induced Cognitive Impairment (Chemobrain): Restraining Its Neuroinflammation, Synaptic Dysplasticity and Apoptosis

et al. 2022

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Chemo fog is one of the most serious health concerns encountered by cancer survivors receiving doxorubicin (DOX)-based chemotherapy. Oxidative stress, neuroinflammation, apoptosis and impairment of synaptic plasticity are regarded as the key factors implicated in DOX-induced cognitive impairment. This research aimed to assess the possible neuroprotective effect of cerium oxide nanoparticles (CeNPs) against DOX-induced neurotoxicity. Forty-eight rats were divided into four groups (12 rats/group): control group, CeNPs group (received oral CeNPs solution (35 mg/kg) daily for 4 weeks), and DOX group (were administered DOX intraperitoneally (2 mg/kg, once/week for 4 weeks)) and DOX+ CeNPs group. The findings revealed that CeNPs mitigated behavioral alterations in DOX-induced cognitive deficit. Additionally, CeNPs alleviated the histopathological abnormalities in hippocampus and ameliorated DOX-induced neuroinflammation by downregulating the expression of NF-κB, TNF-α, IL-1β and IL6. In addition, CeNPs antagonized the apoptosis through reducing the protein expression of cytochrome c and caspase 3. In addition, it stimulated the antioxidant defense, as indicated by upregulating the expression of the Nrf2, HO-1 and PGC-1α genes. CeNPs improved synaptic plasticity via acting on the BDNF. These actions were related through the modification of SIRT-1 expression. Based on the aforementioned results, CeNPs antagonized the doxorubicin-induced neurodegeneration by its antioxidant, anti-inflammatory and antiapoptotic effects, alongside its SIRT-1 mediated mechanisms.

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Light and Electron Microscopic Study on the Possible Protective Effect of Nigella Sativa Oil on Cisplatin Hepatotoxicity in Albino Rats

Cited by 2 publications

References 25 publications

Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Activation of SIRT-1 Pathway by Nanoceria Sheds Light on Its Ameliorative Effect on Doxorubicin-Induced Cognitive Impairment (Chemobrain): Restraining Its Neuroinflammation, Synaptic Dysplasticity and Apoptosis

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