Light and electron microscopic study of the toxic effect of prolonged lead exposure on the seminiferous tubules of albino rats and the possible protective effect of ascorbic acid

Shafai, Adel El; Zohdy, Nahed; Mulla, Khaled El; Hassan, Manal; Morad, Noha

doi:10.1016/j.fct.2010.11.033

Cited by 40 publications

(24 citation statements)

References 34 publications

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“…lead acetate for 3 months, loss of normal architecture of the testicular tissue in the form of thin walled seminiferous tubules with wide lumen and vacuolation in the spermatogenic epithelium mostly separating primary spermatocytes from spermatogonia and surrounding nuclei of sertoli cells was noticed. Also apoptotic bodies were found among the basal part of the spermatogenic epithelium (El-Shafai et al, 2011). Oral administration of 15 mg/kg b.w., 20 mg/kg b.w.…”

Section: Introductionmentioning

confidence: 99%

The ameliorative effects of DMSA and some vitamins against toxicity induced by lead in the testes of albino rats. II

El-Sayed

Abdel-Ghafar

Adly

et al. 2015

The Journal of Basic & Applied Zoology

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Lead is a poison that affects virtually every system in the body. The current study was planned to examine the toxic effects of lead acetate on the histological picture of testes, and the protective roles of DMSA, combined vitamins C and E, and DMSA combined with vitamin C plus vitamin E against the histopathological changes in the testes of albino rats induced by lead acetate. Oral administration of lead acetate caused necrosis of spermatogenic cells in the seminiferous tubules, congestion of interstitial blood vessels, severe interstitial edema and complete necrosis in the seminiferous tubules. Co-administration of DMSA with lead acetate minimized the histopathological changes exhibited by lead acetate in the affected organ compared with lead acetateintoxicated rats. Lead acetate combined with vitamin C plus vitamin E supplemented rats showed mild congestion of the interstitial blood vessels and the seminiferous tubules with its components appeared normal compared to DMSA treated rats. Treatment with DMSA combined with vitamin C plus vitamin E showed more or less normal histological appearance of the testes in lead acetate induced histopathological changes in the affected organ. These results show that DMSA, as a chelating agent for lead, and the combination of vitamins C and E as antioxidants reduced the toxic effects of lead on the histological structure of testes in albino rats but did not provide complete protection. Whereas, the supplementation of DMSA combined with both vitamins C and E provide complete protection against toxicity induced by lead in the testes of albino rats. ª 2015 The Egyptian German Society for Zoology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Section: Introductionmentioning

confidence: 99%

The ameliorative effects of DMSA and some vitamins against toxicity induced by lead in the testes of albino rats. II

El-Sayed

Abdel-Ghafar

Adly

et al. 2015

The Journal of Basic & Applied Zoology

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“…The possible protective effect of ascorbic acid (vitamin C) from the toxic effects of prolonged Pb exposure on testicular seminiferous tubules of albino rats was reported (Shafai et al 2011). A loss of normal testicular tissue, where the seminiferous tubules were in the form of thin-walled tubes with a wide lumen, and the appearance of cell vacuolation of germinal epithelium separated from the Sertoli cells were noted (Shafai et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…A loss of normal testicular tissue, where the seminiferous tubules were in the form of thin-walled tubes with a wide lumen, and the appearance of cell vacuolation of germinal epithelium separated from the Sertoli cells were noted (Shafai et al 2011). An ultrastructural analysis revealed degenerating group of cells with cytoplasmic vacuolization, apoptotic cells with heterochromatic nuclei with dense cytoplasm and deformed spermatozoa head and extension of subacrosomal space (Shafai et al 2011). Another group of researchers observed a significant reduction in the weight of reproductive organs, reduced epididymal spermatozoa count, and reduced number of motile and viable spermatozoa in rats exposed to Pb with reduction in spermatozoa production, impaired spermatozoa quality, and a significant decrease of serum testosterone levels (Anjum et al 2011).…”

Section: Discussionmentioning

confidence: 99%

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Lead induced alterations in rabbit spermatozoa motility and morphology in vitro

Kročková¹,

Roychoudhury²,

Slanina³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The aim of this in vitro study was to determine the effect of lead chloride (PbCl 2 ) on rabbit spermatozoa motility and morphology. Lead concentrations in the medium ranged between 0.45 and 11.17 μg/ml; incubation time was 240 min (analyzed immediately after Pb addition followed by 30, 60, 120, 180, and 240 min), and temperatures of the culture environment were 22°C (laboratory), 4°C (refrigerator), and 37°C (incubator). Results were compared with a control group without Pb addition. After 30 min of culture at 22°C, a negative effect of Pb was noted as spermatozoa motility significantly decreased in groups with higher concentrations. After 120 and 240 min, a dose-dependent effect on spermatozoa motility was noted. At 4°C, spermatozoa motility analysis detected no significant differences between any of the experimental groups and control. At 37°C, a negative effect of Pb incubation on motility was detected at Times 30, 60, 120, 180, and 240 in groups with high concentrations. At Times 120, 180, and 240 a significant decrease in spermatozoa motility was also noted in all experimental groups in comparison to control. The analysis of pathological spermatozoa at Time 240 revealed an increasing trend of morphological abnormalities after incubation with Pb. Across three temperature regimes an increase of morphological changes was noted, particularly in the group with the highest Pb concentration. The predominant morphological abnormalities were knob twisted flagellum, flagellum ball, separated flagellum, and broken flagellum. Knob twisted flagella represented the most frequent pathological changes in the experimental group with the highest Pb concentration. Results suggest that the inhibitory effect of Pb on spermatozoa motility parameters depends on the concentration, incubation time, as well as environmental temperature during incubation. Furthermore, a negative effect of Pb in vitro on spermatozoa morphology indicates possible reproductive problems under in vivo conditions, too.

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“…Lead is another heavy metal known to induce alterations in the male reproductive tract, particularly by reducing steroid metabolic activity in SCs and disrupting SC:germ cells co-cultures [87,88]. In vivo experiments have shown that lead induces mitochondrial changes in SCs [89] and alters the normal testicular structure [90]. p,p -dichlorodiphenoxydichloroethylene (p,p'-DDE), which is a metabolite of dichlorodiphenoxytrichloroethane (DTT), has been shown to increase key regulators of germ cell apoptosis (e.g.…”

Section: Relevance Of Metabolic Cooperation In Testis In Pharmacologimentioning

confidence: 99%

Metabolic Cooperation in Testis as a Pharmacological Target: From Disease to Contraception

Alves¹,

Dias²,

Silva³

et al. 2015

CMP

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Abstract:The development of a "male pill" to control fertility is still a major challenge. Although women have several options to play an active role in the couple family planning, men are very limited in terms of contraceptive methods. Several approaches have been proposed to develop a male contraceptive and can be divided in two major groups: hormonal and non-hormonal methods. Within the testis, the somatic Sertoli cell (SC) is known as the "nurse cell" since it provides the physical and nutritional support for the developing germ cells. Moreover, adjacent SCs form the Sertoli/blood testis barrier (BTB), which divides the seminiferous epithelium into the basal and the apical compartments, controlling the passage of substances to the site where germ cells develop. Among the several functions of SC, its metabolism and the production of lactate, acetate and other metabolic factors are essential for the normal occurrence of spermatogenesis. In the last years, several works have highlighted that the metabolic cooperation established between SCs and developing germ cells is compromised in several diseases associated with male subfertility/infertility. Notably, several metabolismassociated proteins are specifically expressed in the testis. Thus, there are several evidences illustrating that the control of male fertility can be achieved by targeting testicular cells metabolism. Herein, we discuss the metabolic cooperation in testis as a potential pharmacological target to counteract subfertility/infertility promoted by several diseases, particularly metabolic diseases. We also discuss how it can contribute to the development of a male contraceptive.

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Light and electron microscopic study of the toxic effect of prolonged lead exposure on the seminiferous tubules of albino rats and the possible protective effect of ascorbic acid

Cited by 40 publications

References 34 publications

The ameliorative effects of DMSA and some vitamins against toxicity induced by lead in the testes of albino rats. II

The ameliorative effects of DMSA and some vitamins against toxicity induced by lead in the testes of albino rats. II

Lead induced alterations in rabbit spermatozoa motility and morphology in vitro

Metabolic Cooperation in Testis as a Pharmacological Target: From Disease to Contraception

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