Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Rich, Peter R.; Heathcote, Peter

doi:10.1016/0005-2728(83)90207-4

Cited by 19 publications

(2 citation statements)

References 20 publications

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“…The work represents the first step of a more elaborated and ambitious goal, which we have designed and that is currently under scrutiny in our laboratory (for preliminary reports, see refs. 27 , 28 ).…”

Section: The Need Of Implementing An Energy-production Module In Syntmentioning

confidence: 99%

Novel directions in molecular systems design: The case of light-transducing synthetic cells

Stano

Altamura

Mavelli

2017

Communicative & Integrative Biology

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Important progresses have been achieved in the past years in the field of bottom-up synthetic biology, especially aiming at constructing cell-like systems based on lipid vesicles (liposomes) entrapping both biomolecules or synthetic compounds. These “synthetic cells” mimic the behaviour of biological cells but are constituted by a minimal number of components. One key aspect related to this research is the energetic needs of synthetic cells. Up to now, high-energy compounds have been given in order to drive biochemical reactions inside the vesicle lumen. In order to be autonomous, synthetic cells must produce their own biochemical energy from available energy sources. At this aim we started a long-term research program focused on the construction of photoautotrophic synthetic cells, starting with the reconstitution, in active and highly oriented form, of the photosynthetic reaction centre in giant lipid vesicles (Altamura et al., PNAS 2017, 114, 3837–3842). Here we comment this first milestone by showing the synthetic biology context wherein it is developed, the future steps, and the experimental approach that might allow such an achievement.

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Section: The Need Of Implementing An Energy-production Module In Syntmentioning

confidence: 99%

Novel directions in molecular systems design: The case of light-transducing synthetic cells

Stano

Altamura

Mavelli

2017

Communicative & Integrative Biology

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“…Before purification, lipid-associated integral membrane proteins must be separated from the lipid membrane components. This step, termed solubilization, is commonly achieved with amphiphilic, anionic or nonionic detergents such as N,N -dimethyldodecylamine N -oxide (LDAO), cholate, n -octyl-β -D -glucopyranoside (OGP), β -dodecyl-maltoside or Triton ® X-100 (5,12,14,17). Detergents usually must be present during each purification step to maintain both protein solubility and functional activity but should be removed from the reconstituted proteoliposomes.…”

Section: Introductionmentioning

confidence: 99%

Purification and Reconstitution of an Integral Membrane Protein, the Photoreaction Center of Rhodobacter sphaeroides , Using Synthetic Sugar Esters

et al. 2000

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Detergents are indispensable reagents for the extraction and solubilization of integral membrane proteins, but their removal from a reconstituted phospholipid-protein complex is usually desirable. In this paper, we describe a novel method in which the synthetic sugar esters 6-O-octanoyl-beta-D-glucose (OG) or 6-O-octanoyl-beta-D-mannose (OM) are used as detergents for both the isolation and the rapid reconstitution of the photosynthetic reaction center protein of Rhodobacter sphaeroides. Following solubilization of the reaction center with OG or OM and reconstitution of this protein in liposomes, a convenient removal of these detergents was achieved within less than two hours by hydrolytic cleavage of the sugar esters using immobilized lipases. Best results were achieved with lipase from Bacillus sp. immobilized on silica gel.

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Towards Synthetic Cells with Self‐Producing Energy

Hwang,

Kim,

Liu

2024

ChemPlusChem

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Autonomous generation of energy, specifically adenosine triphosphate (ATP), is critical for sustaining the engineered functionalities of synthetic cells constructed from the bottom‐up. In this mini‐review, we categorize studies on ATP‐producing synthetic cells into three different approaches: photosynthetic mechanisms, mitochondrial respiration mimicry, and utilization of non‐conventional approaches such as exploiting synthetic metabolic pathways. Within this framework, we evaluate the strengths and limitations of each approach and provide directions for future research endeavors. We also introduce a concept of building ATP‐generating synthetic organelle that will enable us to mimic cellular respiration in a simpler way than current strategies. This review aims to highlight the importance of energy self‐production in synthetic cells, providing suggestions and ideas that may help overcome some longstanding challenges in this field.

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Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Cited by 19 publications

References 20 publications

Novel directions in molecular systems design: The case of light-transducing synthetic cells

Novel directions in molecular systems design: The case of light-transducing synthetic cells

Purification and Reconstitution of an Integral Membrane Protein, the Photoreaction Center of Rhodobacter sphaeroides , Using Synthetic Sugar Esters

Towards Synthetic Cells with Self‐Producing Energy

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