Light-Activated Nanodevice for On-Demand Imaging of miRNA in Living Cells via Logic Assembly

Zhou, Huimin; Jiang, Yao; Zhao, Wenjing; Zhang, Shusheng

doi:10.1021/acsami.2c00376

Cited by 18 publications

(7 citation statements)

References 37 publications

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“…By controllably releasing HCR hairpins with high local concentrations to efficiently trigger HCR and further recruiting the long dsDNA polymers, this method contributed to highly sensitive and accurate miRNA imaging in living cells. [116,117] CHA, as a typical enzyme-free amplification technique, promotes catalyzed hybridization of hairpins and produces numerous short dsDNA products. [8,48] By extending the stem of a conventional DNA hairpin to form a dumbbell structure and introducing a PC linker into the extended segment, Luo et al designed a UV light-responsive CHA amplicon (Figure 2B).…”

Section: Photolysis-activated Dna Circuits For Molecular Imagingmentioning

confidence: 99%

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Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

Shang

et al. 2023

Advanced Sensor Research

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Probing endogenous molecules in living entities is significant to help decipher biological functions and exploit novel theranostics. DNA circuits that can recognize molecular inputs of interest and transduce them into readable signal outputs in an isothermal and autonomous manner have been actively pursued as versatile toolkits for intracellular biosensing research. Tremendous efforts are being devoted to developing integrated DNA circuits with high sensitivity, while spatiotemporal selectivity is often overlooked in the construction of functional DNA circuitry systems. This requires the development of stimuli‐responsive DNA circuits that can be activated on‐demand from the initial sensing‐blunt state to the sensing‐ready state under a programmable manner, for achieving precise bioimaging with high spatiotemporal control. In this review, an overview of recent advances in the construction of stimuli‐responsive DNA circuits that respond to particular triggers, including external physical stimuli and endogenous biological cues, and their spatiotemporally controllable molecular bioimaging applications is provided. The current challenges and potential solutions of these stimuli‐responsive DNA circuits for their future developments in this emerging field are also discussed.

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Section: Photolysis-activated Dna Circuits For Molecular Imagingmentioning

confidence: 99%

Section: External Stimuli‐responsive Dna Circuits For Bioimagingmentioning

confidence: 99%

Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

Shang

et al. 2023

Advanced Sensor Research

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“…The DNA-AuNPs’ application in the biomedical field has emerged since 1996 with the landmark work for attaching thiol DNA to AuNPs, which was proposed by Mirkin et al (1996) This proposed strategy made numerous applications of DNA-AuNPs possible, including the preparation of biosensing ( Pan et al, 2019 ), bioimaging ( Sun et al, 2019a ; Zhou et al, 2022b ), disease diagnosis ( Lin et al, 2019 ), and drug delivery ( Zheng et al, 2019 ). In brief, the simple and efficient modification methods of DNA-based AuNPs play a crucial role in all these applications ( Degliangeli et al, 2014 ; Peng et al, 2022 ).…”

Section: Dna Modification Of Aunpsmentioning

confidence: 99%

DNA-functionalized gold nanoparticles: Modification, characterization, and biomedical applications

Luo

et al. 2022

Front. Chem.

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With the development of technologies based on gold nanoparticles (AuNPs), bare AuNPs cannot meet the increasing requirements of biomedical applications. Modifications with different functional ligands are usually needed. DNA is not only the main genetic material, but also a good biological material, which has excellent biocompatibility, facile design, and accurate identification. DNA is a perfect ligand candidate for AuNPs, which can make up for the shortcoming of bare AuNPs. DNA-modified AuNPs (DNA-AuNPs) have exciting features and bright prospects in many fields, which have been intensively investigated in the past decade. In this review, we summarize the various approaches for the immobilization of DNA strands on the surface of AuNPs. Representative studies for biomedical applications based on DNA-AuNPs are also discussed. Finally, we present the challenges and future directions.

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“…In addition, the THMS structure can keep the aptamer sequence in a free state, thereby maintaining the binding affinity and specificity of the aptamer and even achieving higher sensitivity . Considering these remarkable features, the THMS-based aptasensor for hazard residue analysis in food has been reported by many scholars. − However, thus far, these works rarely introduce signal amplification strategies, leaving room for improvement in ultrasensitive analysis. Deoxyribozyme (DNAzyme) has been proven to catalyze many of the same reactions as protein enzymes, including RNA/DNA-cleavage, ligation, phosphorylation, etc .…”

Section: Introductionmentioning

confidence: 99%

Triple-Helix Molecular Switch Triggered Cleavage Effect of DNAzyme for Ultrasensitive Electrochemical Detection of Chloramphenicol

Wang

Ren

et al. 2022

ACS Appl. Mater. Interfaces

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The abuse of chloramphenicol (CAP) in animal-derived products leads to serious food safety problems, so the sensitive and accurate determination of CAP residues has great noteworthiness for public health. Herein, we present a novel electrochemical aptasensor that incorporates a poly(diallyldimethylammonium chloride) functionalized graphene/Ag@Au nanosheets (PDDA-Gr/Ag@Au NSs) composite modified electrode and a DNAzyme signal amplification effect triggered by a triple-helix molecular switch (THMS) for detecting CAP. The PDDA-Gr/Ag@Au NSs composite has the advantages of high surface area, great conductivity, and dispersibility and has successfully improved the electrochemical performance of the electrode. Specific interaction with CAP will cause the signal transduction probe (STP) to be released from the THMS. After that, the DNAzyme will be activated with the help of Pb2+ and remove the immobilized signal probe on the electrode surface. The signal change was recorded by square wave voltammetry (SWV) and led to an accurate quantification of CAP. With all these features, the proposed sensing strategy yielded a satisfactory analytical performance with linearity between 1 pM and 1 μM and a limit of detection of 18.6 fM. Furthermore, the aptasensor shows excellent specificity for CAP in the presence of other antibiotics and resists interference with other common metal ions. Importantly, the performance is not diminished when the constructed aptasensor is applied to measuring CAP in milk powder. This THMS-based method is easy to design, and alteration to different targets can be achieved by simply replacing the aptamer sequence in the THMS. Therefore, this method shows significant prospects as a flexible platform for accurate monitoring of antibiotic residues in foodstuffs.

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Light-Activated Nanodevice for On-Demand Imaging of miRNA in Living Cells via Logic Assembly

Cited by 18 publications

References 37 publications

Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

DNA-functionalized gold nanoparticles: Modification, characterization, and biomedical applications

Triple-Helix Molecular Switch Triggered Cleavage Effect of DNAzyme for Ultrasensitive Electrochemical Detection of Chloramphenicol

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