Ligand-directed tosyl chemistry for in situ native protein labeling and engineering in living systems: from basic properties to applications

Tsukiji, Shinya; Hamachi, Itaru

doi:10.1016/j.cbpa.2014.07.012

Cited by 57 publications

(45 citation statements)

References 55 publications

(55 reference statements)

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“…Thus, a synthetic system that can detect endogenous proteins and control gene expression in a living cell provides a useful tool for biological and therapeutic applications. The existing techniques to detect-specific proteins, including western blotting, immunostaining, LC-MS (4), chemical probes (5), proximity ligation (6) and tagging the protein of interest with reporter signals (7) (e.g. fluorescent proteins), enable us to analyze protein expression levels and cell states.…”

Section: Introductionmentioning

confidence: 99%

Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells

Kawasaki

Fujita

Nagaike

et al. 2017

Nucleic Acids Research

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Synthetic biology has great potential for future therapeutic applications including autonomous cell programming through the detection of protein signals and the production of desired outputs. Synthetic RNA devices are promising for this purpose. However, the number of available devices is limited due to the difficulty in the detection of endogenous proteins within a cell. Here, we show a strategy to construct synthetic mRNA devices that detect endogenous proteins in living cells, control translation and distinguish cell types. We engineered protein-binding aptamers that have increased stability in the secondary structures of their active conformation. The designed devices can efficiently respond to target proteins including human LIN28A and U1A proteins, while the original aptamers failed to do so. Moreover, mRNA delivery of an LIN28A-responsive device into human induced pluripotent stem cells (hiPSCs) revealed that we can distinguish living hiPSCs and differentiated cells by quantifying endogenous LIN28A protein expression level. Thus, our endogenous protein-driven RNA devices determine live-cell states and program mammalian cells based on intracellular protein information.

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Section: Introductionmentioning

confidence: 99%

Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells

Kawasaki

Fujita

Nagaike

et al. 2017

Nucleic Acids Research

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“…The recently developed affinity labelling chemistry holds great potentialf or better controlled reactions. [93,94] The chemical functionality repertoire of cells could be further expanded by using other strategies, such as metabolic engineering, unnatural amino acid incorporation,a nd enzymatice ngineering. These approaches, which are complementaryt oo ne another, provide us great opportunities to manipulate the cell surface to regulate their fate or function.…”

Section: Discussionmentioning

confidence: 99%

Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

Yin

Guanbang

et al. 2018

Chemistry A European J

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The cell surface serves important functions such as the regulation of cell-cell and cell-environment interactions. The understanding and manipulation of the cell surface is important for a wide range of fundamental studies of cellular behavior and for biotechnological and medical applications. With the rapid advance of biology, chemistry and materials science, many strategies have been developed for the functionalization of bacterial and mammalian cell surfaces. Here, we review the recent development of chemical and enzymatic approaches to cell surface engineering with particular emphasis on discussing the advantages and limitations of each of these strategies.

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“…The ability to functionalize al igand that selectively targets a protein of interestw ith ac atalyst to direct chemistry has attracted significant attention. [21,22] The Nakamura group investigated the use of [Ru(bpy) 3 ] 2 + conjugates to direct the oxidation of tyrosine residues and coupling with tyrosyl radical trapping agents( Figure3). It was shownt hat ap henylenediamine performed best (25 alternatives tested) and it could be func-Simona Angerani studied chemistry at the University of Milan where she obtained her Bachelor'sa nd Master's degree.…”

Section: Oxidative Receptor Labeling Reactionsmentioning

confidence: 99%

Visible Light Photoredox Catalysis Using Ruthenium Complexes in Chemical Biology

Angerani

Winssinger

2019

Chemistry A European J

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Ligand-directed tosyl chemistry for in situ native protein labeling and engineering in living systems: from basic properties to applications

Cited by 57 publications

References 55 publications

Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells

Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells

Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

Visible Light Photoredox Catalysis Using Ruthenium Complexes in Chemical Biology

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