Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

Bi, Xiaobao; Yin, Juan; Guanbang, Ashley Chen; Liu, Chuan‐Fa

doi:10.1002/chem.201705049

Cited by 24 publications

(37 citation statements)

References 113 publications

(178 reference statements)

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“…However, dynamic surface glycan recycling and inevitable cell division processes ultimately restrict the timeframe for appreciable surface reactivity. This issue raises concerns about whether long‐term feeding of cells with unnatural sugars can lead to unexpected effects on cell and microtissue development . Moreover, the importance of spacers on the stability of such cell‐networks requires further studies.…”

Section: Cell‐rich Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Section: Cell‐rich Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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“…Over the past decade, there is growing interest in the chemical community to develop synthetic tools that can install chemical and biological handles onto different types of cells to replace or assist natural interactions occurring on the cell surface. 7 In the case of mammalian cells, one common approach is to modify pericellular matrix or surface proteins via non-covalent or covalent reactions. 8 Examples include the use of natural functional groups (primary amine, terminal thiol, and carboxyl), 9 metabolic engineering of unnatural amino sugar analogs as reactive tags, 10,11 and insertion of functional motifs with hydrophobic anchors into the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Engineering bacterial surface interactions using DNA as a programmable material

Kong

et al. 2022

Chem. Commun.

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“…We have recently developed protein binders based on ODN-small-molecule conjugates [21][22][23] and have shown that such binders can be designed to reversibly interact with different proteins 21,22 and even mediate unnatural protein-protein communication processes 21 . Parallel to these efforts, much progress has been made in the ability to modify cell surfaces with synthetic agents that bear unique functionalities [28][29][30][31] . The synthetic agents can be designed to selectively target and be covalently attached to natural CSPs [32][33][34][35][36] .…”

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confidence: 99%

Decorating bacteria with self-assembled synthetic receptors

et al. 2020

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The responses of cells to their surroundings are mediated by the binding of cell surface proteins (CSPs) to extracellular signals. Such processes are regulated via dynamic changes in the structure, composition, and expression levels of CSPs. In this study, we demonstrate the possibility of decorating bacteria with artificial, self-assembled receptors that imitate the dynamic features of CSPs. We show that the local concentration of these receptors on the bacterial membrane and their structure can be reversibly controlled using suitable chemical signals, in a way that resembles changes that occur with CSP expression levels or posttranslational modifications (PTMs), respectively. We also show that these modifications can endow the bacteria with programmable properties, akin to the way CSP responses can induce cellular functions. By programming the bacteria to glow, adhere to surfaces, or interact with proteins or mammalian cells, we demonstrate the potential to tailor such biomimetic systems for specific applications.

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Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering

Cited by 24 publications

References 113 publications

Advanced Bottom‐Up Engineering of Living Architectures

Advanced Bottom‐Up Engineering of Living Architectures

Engineering bacterial surface interactions using DNA as a programmable material

Decorating bacteria with self-assembled synthetic receptors

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