Ligand Coupling and Decoupling Modulates Stem Cell Fate

Abstract

In natural microenvironment, various proteins containing adhesive ligands in fibrous and non‐fibrous structures dynamically couple and decouple to regulate stem cell fate. Herein, materials presenting movably couplable ligands are developed by grafting liganded gold nanoparticles (AuNPs) to a substrate followed by flexibly grafting liganded movable linear nanomaterials (MLNs) to the substrate via a long bendable linker, thereby creating a space between the MLNs and the AuNPs in the decoupled state. Magnetic control of the MLNs decreases this space via the bending of the linker to couple the MLNs to the AuNPs. Remote control of ligand coupling stimulates integrin recruitment to the coupled ligands, thereby non‐toxically facilitating the focal adhesion, mechanosensing, and potential differentiation of stem cells, which is suppressed by ligand decoupling. Versatile tuning of size, aspect ratio, distributions, and ligands of the MLNs can help to decipher dynamic ligand‐coupling‐dependent stem cell fate to advance regenerative therapies.

Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging

“…Precise targeting of cells in which to induce ferroptosis is an essential consideration to effectively use ferroptosis for cancer treatment, and magnetically responsive NPs can be used to achieve this [ 156 ]. Various magnetic field types can precisely steer the MNPs to the targeted region [ 157 ].…”

Cancer Cell Membrane‐Based Materials for Biomedical Applications

“…Dynamic materials are designed to manipulate the presentation of ligands through remotely activated signals to regulate cell adhesion. [208] By combining longitudinal, real-time, and in-depth information from PAI with surfacelevel images from FL imaging and an overall view of the entire brain from MRI, these imaging techniques enable the identification of whole-brain tumors. The cRGD-CM-CPIO nanocomposites would help surgeons with MRI for surgical planning, FI and PAI for real-time tumor identification, FI for evaluating surgical outcomes, and FI for margin validation.…”

Photonic control of ligand nanospacing in self-assembly regulates stem cell fate

“…Diverse materials have been designed to realize remote manipulability [ [22] , [23] , [24] , [25] ] of cell-material interplay via magnetic field [ [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] ] or ultraviolet (UV)-free light [ 20 , [39] , [40] , [41] , [42] , [43] , [44] , [45] ] to acquire superior tissue penetrability for in vivo applications. Photoisomers can undergo photoisomerization in response to dual- or single-wavelength light in the range of UV and visible (Vis) light wavelengths [ 46 ], thus enabling the cell regulation [ 47 ].…”