Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from <i>Anacardium occidentale</i> L. in <i>Caenorhabditis elegans</i>

Duangjan, Chatrawee; Rangsinth, Panthakarn; Gu, Xiao-Jie; Wink, Michael

doi:10.1155/2019/9012396

Cited by 61 publications

(94 citation statements)

References 39 publications

(69 reference statements)

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“…The accumulation of age pigment in C. elegans was reduced dose dependently by orange extracts, and the most significant effect in reducing the accumulation of age pigment was shown at 400 mg/mL (decreased by 34.4% compared with the control). Since many reports claimed that the pre-treatment of antioxidants could decrease age pigment accumulation, [3] we inferred that orange extract downregulation of age pigment levels might be associated with its strong antioxidant potential in C. elegans (Figures 5 and 8). In addition, there was no obvious effect in the body length of orange extract-treated and untreated worms.…”

Section: Discussionmentioning

confidence: 94%

“…The results of the transcriptional analysis illustrated that orange extracts activated the expressions of age-1 and daf-16 genes in the IIS pathway, sek-1 and skn-1 genes in the MAPK pathway, and antioxidant genes, including sod-3 and gst-4 ( Figure 9B). The IIS pathway is a highly conservative and elaborate lifetime regulator, which plays a key role in development, metabolism, and aging in C. elegans [3]. This signaling pathway is activated by the translocation of DAF-16 to the nucleus [34].…”

Section: Discussionmentioning

confidence: 99%

“…Previous research has indicated that increased consumption of fruit and vegetables can effectively reduce the risk of some functional declines associated with aging, which is associated with the additive and synergistic interactions among phytochemicals existing in whole fruit and vegetables [2]. Bioactive non-nutrient plant phytochemicals, especially phenolicsand flavonoids, have been linked to many biological properties, such as antioxidant, anticancer, and anti-inflammatory properties, which could be potential sources of anti-aging agents due to their excellent biological activities [3].…”

Section: Introductionmentioning

confidence: 99%

“…A previous report showed that phytochemicals extracted from blueberry could promote stress tolerance and longevity via dauer formation (DAF)-16 in C. elegans [11]. Anacardium occidentale leaf extracts containing a number of polyphenolic antioxidants enhanced the survival rate of nematodes under oxidative stress via the DAF-16/FOXO (Forkhead box O) and SKN-1/Nrf-2 (Nuclear factor erythroid 2-related factor 2) signaling pathways [3]. 4-Hydroxybenzoic acid isolated from Veronica peregrina prolonged the lifespan of C. elegans via SIR-2.1/SIR2-mediated DAF-16/FOXO activation, independent of dietary restriction and the IIS pathway [12].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

et al. 2020

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Orange, with various bioactive phytochemicals, exerts various beneficial health effects, including anti-cancer, antioxidant, and anti-inflammatory properties. However, its anti-aging effects remain unclear. In this study, the Caenorhabditis elegans (C. elegans) model was used to evaluate the effects of orange extracts on lifespan and stress resistance. The results indicated that orange extracts dose-dependently increased the mean lifespan of C. elegans by 10.5%, 18.0%, and 26.2% at the concentrations of 100, 200, and 400 mg/mL, respectively. Meanwhile, orange extracts promoted the healthspan by improving motility, and decreasing the accumulation of age pigment and intracellular reactive oxygen species (ROS) levels without damaging fertility. The survival rates of orange extract-fed worms were obviously higher than those of untreated worms against thermal and ultraviolet-B (UV-B) stress. Moreover, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly enhanced while malondialdehyde (MDA) contents were diminished. Further investigation revealed that worms supplemented with orange extracts resulted in upregulated levels of genes, including daf-16, sod-3, gst-4, sek-1, and skn-1, and the downregulation of age-1 expression. These findings revealed that orange extracts have potential anti-aging effects through extending the lifespan, enhancing stress resistance, and promoting the healthspan.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

et al. 2020

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“…The chemical constituents of LRH were analysed by GC-MS, then the chromatographic peaks were identified by comparison to the NIST library. The chromatogram of LRH exhibited about 50 isolated peaks, the identified compounds that provided a match factor and/or a reverse match factor greater than 800 20 are shown in the supplementary data 2. The identified compounds could be classified into four major groups which were alkanes (32.6%), fatty acids (29.8%), terpenes (21.8%), and ketones (15.8%).…”

Section: Resultsmentioning

confidence: 99%

HIV-1 protease and reverse transcriptase inhibition by tiger milk mushroom (Lignosus rhinocerus) sclerotium extracts: In vitro and in silico studies

Sillapachaiyaporn

Chuchawankul

2020

Journal of Traditional and Complementary Medicine

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Background and aim Lignosus rhinocerus (LR) is an edible mushroom with a variety of medicinal properties such as neurostimulation, immunomodulation, anti-inflammation, anti-oxidation, anti-proliferation, anti-diabetes and especially antiviral activity. Human immunodeficiency virus type-1 (HIV-1) needs the HIV-1 protease (PR) and reverse transcriptase (RT) for its replication. Therefore, both HIV-1 PR and RT are important targets for antiretroviral drug development. Experimental procedure The crude hexane (LRH), ethanol (LRE) and water (LRW) extracts of LR were in vitro screened for inhibitory activity against HIV-1 PR and RT, then anti-HIV-1 activity on the infected MOLT-4 cells were determined. Chemical constituents of the extracts were identified by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC)-MS. The identified compounds were in silico analysed for drug-likeness property and molecular modelling. Results and conclusion According to our screening assays, LRE and LRW significantly inhibited both enzymes (25–55%), while LRH suppressed only the HIV-1 PR activity (88.97%). At 0.5 mg/ml of LRW showed significant inhibition of HIV-1 induced syncytial formation and p24 production in the infected MOLT-4 cells. Investigation of chemical analysis revealed that major groups of identified constituents found in the extracts were fatty acids, peptides and terpenoids. In silico analysis showed that heliantriol F and 6 alpha-fluoroprogesterone displayed great binding energies with HIV-1 PR and HIV-1 RT, respectively. These findings suggest that LR could be a potential source of compounds to inhibit HIV-1 PR and/or RT activities in vitro . Furthermore, our results provide beneficial data for the development of novel HIV-1 PR and RT inhibitors.

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Acute, reproductive, and developmental toxicity of essential oils assessed with alternative in vitro and in vivo systems

et al. 2020

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Essential oils (EOs) have attracted increased interest for different applications such as food preservatives, feed additives and ingredients in cosmetics. Due to their reported variable composition of components, they might be acutely toxic to humans and animals in small amounts. Despite the necessity, rigorous toxicity testing in terms of safety evaluation has not been reported so far, especially using alternatives to animal models. Here, we provide a strategy by use of alternative in vitro (cell cultures) and in vivo (Caenorhabditis elegans, hen’s egg test) approaches for detailed investigation of the impact of commonly used rosemary, citrus and eucalyptus essential oil on acute, developmental and reproductive toxicity as well as on mucous membrane irritation. In general, all EOs under study exhibited a comparable impact on measured parameters, with a slightly increased toxic potential of rosemary oil. In vitro cell culture results indicated a concentration-dependent decrease of cell viability for all EOs, with mean IC50 values ranging from 0.08 to 0.17% [v/v]. Similar results were obtained for the C. elegans model when using a sensitized bus-5 mutant strain, with a mean LC50 value of 0.42% [v/v]. In wild-type nematodes, approximately tenfold higher LC50 values were detected. C. elegans development and reproduction was already significantly inhibited at concentrations of 0.5% (wild-type) and 0.1% (bus-5) [v/v] of EO, respectively. Gene expression analysis revealed a significant upregulation of xenobiotic and oxidative stress genes such as cyp-14a3, gst-4, gpx-6 and sod-3. Furthermore, all three EOs under study showed an increased short-time mucous membrane irritation potential, already at 0.5% [v/v] of EO. Finally, GC–MS analysis was performed to quantitate the relative concentration of the most prominent EO compounds. In conclusion, our results demonstrate that EOs can exhibit severe toxic properties, already at low concentrations. Therefore, a detailed toxicological assessment is highly recommended for each EO and single intended application.

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Lifespan Extending and Oxidative Stress Resistance Properties of a Leaf Extracts from Anacardium occidentale L. in Caenorhabditis elegans

Cited by 61 publications

References 39 publications

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

HIV-1 protease and reverse transcriptase inhibition by tiger milk mushroom (Lignosus rhinocerus) sclerotium extracts: In vitro and in silico studies

Acute, reproductive, and developmental toxicity of essential oils assessed with alternative in vitro and in vivo systems

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