Life-Sparing Effect of Human Cord Blood-Mesenchymal Stem Cells in Experimental Acute Kidney Injury

Morigi, Marina; Rota, Cinzia; Montemurro, Tiziana; Montelatici, Elisa; Cicero, Viviana Lo; Imberti, Barbara; Abbate, Mauro; Zoja, Carla; Cassis, Paola; Longaretti, Lorena; Rebulla, Paolo; Introna, Martino; Capelli, Chiara; Benigni, Ariela; Remuzzi, Giuseppe; Lazzari, Lorenza

doi:10.1002/stem.293

Cited by 164 publications

(142 citation statements)

References 72 publications

(119 reference statements)

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“…Similarly, BM-MSCs promoted PCNA expression by the tubular cells which was an index of renal regeneration as well as inhibiting cell apoptosis induced by cisplatin [31] . Furthermore, injection of human cord blood-MSCs activated the pro-survival factor Akt in the PCTs inducing their proliferation as evidenced by the high number of cells positive for PCNA staining [28] .…”

Section: Discussionmentioning

confidence: 99%

“…Thereafter, washed with phosphate buffer three times and incubated in biotinylated rabbit anti mouse IgG solution (1/400) for 20 minutes followed by streptavidin-biotin solution for 30 minutes. The antibody binding sites in the kidney sections were visualized by diaminobenzidine (DAB) and were counterstained with Mayer's hematoxylin, dehydrated, cleared and mounted [28] .…”

Section: Proliferation Assay By Immunohistochemistrymentioning

confidence: 99%

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Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

Sakr

Elhai

Zeid

et al. 2017

Egyptian Journal of Histology

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Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI) proved to be less effective in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex disorder. Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced nephrotoxicity as a model of AKI. Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin injection. Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells were detected in the female kidney tissue by Y chromosome PCR technique. Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.

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Section: Discussionmentioning

confidence: 99%

Section: Proliferation Assay By Immunohistochemistrymentioning

confidence: 99%

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

Sakr

Elhai

Zeid

et al. 2017

Egyptian Journal of Histology

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“…After 10 years of GMP activity, our center can claim to have multidisciplinary experience in the field of regenerative medicine [5][6][7][8][9][10][11][12].…”

Section: Our History and Authorizationmentioning

confidence: 99%

Challenges of Running a GMP Facility for Regenerative Medicine in a Public Hospital

2017

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Advanced therapy medicinal products represent a new generation of medicinal products for regenerative medicine. Since the implementation of the EU regulation for this innovative class of drugs, the academic and hospital institutions have played a central role in their development and manufacture. For these institutions that are not familiar with the industrial context, being in compliance with the pharmaceutical standards is extremely challenging. This report describes how we dealt with some specific issues during our hospital-based GMP experience. Furthermore, we identify as a future perspective the consistent stimulating contribution that a public entity can ensure for advanced therapy medicinal product development and licensing.

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“…Higher therapeutic efficacy, including complete restoration of kidney function, was observed after infusion of CBMSCs/pericytes compared with regular bone marrow-derived MSCs. 62 However, few donor cells were found in the restored area; also, it was shown in culture and in vivo that the observed renoprotective effects are mediated mainly by angiogenic and antiapoptotic factors secreted by the CBMSCs/pericytes. Another promising source of stem cells is the umbilical cord itself, within which Wharton jelly (a gelatinous substance that insulates umbilical blood vessels) is a recognized source of MSCs.…”

mentioning

confidence: 99%

Perivascular Ancestors of Adult Multipotent Stem Cells

Corselli

Chen

Crisan

et al. 2010

ATVB

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287

209

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Abstract-Independent studies by numerous investigators have shown that it is possible to harvest multipotent progenitor cells from diverse dissociated and cultured fetal, perinatal, and principally adult developed tissues. Despite the increasingly recognized medical value of these progenitor cells, the archetype of which remains the mesenchymal stem cell, this indirect extraction method has precluded the understanding of their native identity, tissue distribution, and frequency. Consistent with other researchers, we have hypothesized that blood vessels in virtually all organs harbor ubiquitous stem cells. We have identified, marked, and sorted to homogeneity by flow cytometry endothelial and perivascular cells in a large selection of human fetal, perinatal, and adult organs. Perivascular cells, including pericytes in the smallest blood vessels and adventitial cells around larger ones, natively express mesenchymal stem cell markers and produce in culture a long-lasting progeny of multilineage mesodermal progenitor cells.

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Life-Sparing Effect of Human Cord Blood-Mesenchymal Stem Cells in Experimental Acute Kidney Injury

Cited by 164 publications

References 72 publications

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

Challenges of Running a GMP Facility for Regenerative Medicine in a Public Hospital

Perivascular Ancestors of Adult Multipotent Stem Cells

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