Life Cycle of the Japanese Green Syllid, Megasyllis nipponica (Annelida: Syllidae): Field Collection and Establishment of Rearing System

Abstract

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Construction of a massive genetic resource by transcriptome sequencing and genetic characterization of <i>Megasyllis nipponica</i> (Annelida: Syllidae)

“…We subsequently washed the algae in plastic trays filled with sea water to release the syllids from the algae into the sea water. The syllid species were identified according to previous studies (San Martín and Worsfold, 2015;Miura et al, 2019). The collected individuals of the focal species were maintained in small plastic containers (84 × 57 × 44 mm) placed in an incubator (21 ± 1 °C, 16-h light and 8-h dark cycle).…”

“…The collected individuals of the focal species were maintained in small plastic containers (84 × 57 × 44 mm) placed in an incubator (21 ± 1 °C, 16-h light and 8-h dark cycle). The detailed rearing conditions were based on Miura et al (2019).…”

“…M. nipponica is widely distributed in intertidal and subtidal zones from Hokkaido to Shikoku, Japan, and is a common annelid species in these areas (Imajima, 1966); sample collection from the field is therefore easy for this species. In addition, rearing and breeding methods for this species have been established recently (Miura et al, 2019), allowing us to observe morphological development and to carry out experiments in the laboratory. However, the genetic information on this species is still insufficient.…”

Secondary‐tail formation during stolonization in the Japanese green syllid, Megasyllis nipponica

“…et Çinar, 2007;De Matos Nogueira & Fukuda, 2008;Glasby & Watson, 2001;Martín & Nishi, 2003;Miura et al, 2019;Okada, 1937;San Martín et al, 2010, 2014Schroeder et al, 2017). Morphological and phylogenetic studies among animals suggested that the developmental processes during fission, fragmentation, and budding may have derived from regeneration multiple times in various clades, including annelids (Bely & Nyberg, 2010;Zattara & Bely, 2011.…”

“…To understand the difference between the processes of secondary-tail formation and posterior regeneration after amputation, detailed intraspecific morphological and histological comparisons during these two processes were carried out in a syllid species, Megasyllis nipponica (Figure 1a,b). This species is widely distributed around Japan and forms a stolon head and a secondary ventral tail during stolonization (Imajima, 1966;Miura et al, 2019). The rearing system in the laboratory is established and the developmental process during stolonization is defined as six stages (S-stage 1-6 in Figure 1c) (Nakamura et al, 2023).…”