Construction of a massive genetic resource by transcriptome sequencing and genetic characterization of &lt;i&gt;Megasyllis nipponica&lt;/i&gt; (Annelida: Syllidae)

Hayashi, Yoshinobu; Oguchi, Kenichi; Nakamura, Mayuko; Koshikawa, Shigeyuki; Miura, Toru

doi:10.1266/ggs.21-00137

Cited by 3 publications

(4 citation statements)

References 65 publications

(95 reference statements)

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“…The rearing system in the laboratory is established and the developmental process during stolonization is defined as six stages (S-stage 1-6 in Figure 1c) (Nakamura et al, 2023). Additionally, genomic resources, such as the complete transcriptome and differentially expressed genes during reproduction are available (Hayashi et al, 2022;Miura et al, 2022;Nakamura et al, 2023).…”

Section: Research Highlightsmentioning

confidence: 99%

Secondary‐tail formation during stolonization in the Japanese green syllid, Megasyllis nipponica

Sato,

Nakamura,

Teresa Aguado

et al. 2024

Evolution and Development

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Benthic annelids belonging to the family Syllidae show a distinctive sexual reproduction mode called “stolonization,” in which posterior segments are transformed into a reproductive individual‐like unit called a “stolon.” Megasyllis nipponica forms a stolon head and a secondary tail in the middle of the trunk before a stolon detaches, while, in the case of posterior amputation, posterior regeneration initiates at the wound after amputation. To understand the difference between posterior regeneration and secondary‐tail formation during stolonization, detailed comparisons between the developmental processes of these two tail‐formation types were performed in this study. Morphological and inner structural observations (i.e., cell proliferation and muscular/nervous development) showed that some processes of posterior regeneration, such as blastema formation and muscular/nervous regeneration at the amputation site, are missing during secondary‐tail formation. In contrast, the secondary tail showed some unique features, such as the formation of ventrolateral half‐tail buds that later fused in the middle and muscle/nerve branches formed before the detachment of the stolon. These novel features in the process of stolonization are suggested to be adaptive since the animals need to recover a posterior end quickly to stolonize again.

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Section: Research Highlightsmentioning

confidence: 99%

Secondary‐tail formation during stolonization in the Japanese green syllid, Megasyllis nipponica

Sato,

Nakamura,

Teresa Aguado

et al. 2024

Evolution and Development

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“…For gene-expression analyses during stolonization, orthologs of candidate genes that were thought to contribute to stolonization via hormonal control, gonadal development, AP axis, or head identi cation were searched in the transcriptome sequence database of M. nipponica (Transcriptome Shotgun Assembly of DDBJ: accession numbers: ICSJ01000001-ICSJ01159970) [23]. The protein sequences of these candidate genes in Platynereis dumerilli from NCBI were used as queries for tBLASTn searches against the M. nipponica dataset.…”

Section: Gene Identi Cationmentioning

confidence: 99%

“…6). The target candidate gene orthologs in the focal species were obtained from the transcriptome data of M. nipponica (Transcriptome Shotgun Assembly of DDBJ: accession numbers: ICSJ01000001-ICSJ01159970) [23].…”

Section: Gene Expression Pro Le During Stolonizationmentioning

confidence: 99%

“…The species Megasyllis nipponica, which is distributed all around Japan in intertidal and subtidal zones [22], has recently been established as a stable laboratory-rearing system [19] and its transcriptome data is also available [23], so that the species has become a model species for further studies. In this study, therefore, morphological and histological observations were carried out to compare stolons and stocks in this species.…”

Section: Introductionmentioning

confidence: 99%

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Morphological, Histological and Gene-Expression Analyses on Stolonization in the Japanese Green Syllid, Megasyllis nipponica (Annelida, Syllidae)

Nakamura,

Oguchi,

Sato

et al. 2023

Preprint

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Benthic annelids belonging to the family Syllidae (Errantia, Phyllodocida) exhibit a unique reproduction mode called “schizogamy” or “stolonization”, in which the posterior body part filled with gametes detaches from the original body, as a reproductive unit (stolon) that autonomously swims and spawns. In this study, detailed developmental processes during stolonization were morphologically/histologically observed in Megasyllis nipponica. The results suggest that the stolon formation started with maturation of gonads, followed by the formation of a head ganglion in the anteriormost segment of the developing stolon. Then, the detailed stolon-specific structures such as stolon eyes and notochaetae were formed. Furthermore, expression profiles of genes involved in the anterior-posterior identity (Hox genes), head identification, germ-line, and hormone regulation were compared between anterior and posterior body parts during the stolonization process. The results reveal that, in the posterior body part, genes for gonadal development were up-regulated, followed by hormone-related genes and head-identification genes. Unexpectedly, Hox genes known to identify body parts along the anterior-posterior axis showed no significant temporal expression changes. Taken together, these findings suggest that during stolonization, gonad development induces the head formation of a stolon, without up-regulation of anterior Hox genes.

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Morphological, histological and gene-expression analyses on stolonization in the Japanese Green Syllid, Megasyllis nipponica (Annelida, Syllidae)

Nakamura,

Oguchi,

Sato

et al. 2023

Sci Rep

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Benthic annelids belonging to the family Syllidae (Annelida, Errantia, Phyllodocida) exhibit a unique reproduction mode called “schizogamy” or “stolonization”, in which the posterior body part filled with gametes detaches from the original body, as a reproductive unit (stolon) that autonomously swims and spawns. In this study, morphological and histological observations on the developmental processes during stolonization were carried out in Megasyllis nipponica. Results suggest that the stolon formation started with maturation of gonads, followed by the formation of a head ganglion in the anteriormost segment of the developing stolon. Then, the detailed stolon-specific structures such as stolon eyes and notochaetae were formed. Furthermore, expression profiles of genes involved in the anterior–posterior identity (Hox genes), head determination, germ-line, and hormone regulation were compared between anterior and posterior body parts during the stolonization process. The results reveal that, in the posterior body part, genes for gonadal development were up-regulated, followed by hormone-related genes and head-determination genes. Unexpectedly, Hox genes known to identify body parts along the anterior–posterior axis showed no significant temporal expression changes. These findings suggest that during stolonization, gonad development induces the head formation of a stolon, without up-regulation of anterior Hox genes.

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Construction of a massive genetic resource by transcriptome sequencing and genetic characterization of <i>Megasyllis nipponica</i> (Annelida: Syllidae)

Cited by 3 publications

References 65 publications

Secondary‐tail formation during stolonization in the Japanese green syllid, Megasyllis nipponica

Secondary‐tail formation during stolonization in the Japanese green syllid, Megasyllis nipponica

Morphological, Histological and Gene-Expression Analyses on Stolonization in the Japanese Green Syllid, Megasyllis nipponica (Annelida, Syllidae)

Morphological, histological and gene-expression analyses on stolonization in the Japanese Green Syllid, Megasyllis nipponica (Annelida, Syllidae)

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