Life cycle of<i>Rickettsia slovaca</i>in L929 cell line studied by quantitative real-time PCR and transmission electron microscopy

Boldiš, Vojtech; Štrus, Jasna; Kocianová, Elena; Tušek‐Žnidarič, Magda; Štefanidesová, Katarína; Schwarzová, K; Kúdelová, M; Sekeyová, Zuzana; Špitalská, Eva

doi:10.1111/j.1574-6968.2009.01510.x

Cited by 10 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). Similar results for Rickettsia slovaca have shown a maximum of multiplication on the second day post-infection for the wild type and on the fourth day for the standard type (Boldis et al 2009). In that case, R. slovaca was propagated in both Vero and L929 cells with similar results (20.3 h/Vero cells and 21.87 h/L929 cells), but R. conorii have been shown to have higher propagation rates when using L929 cells (Balraj et al 2009).…”

Section: Discussionsupporting

confidence: 56%

“…Concerning R. rickettsii (wild type) and R. slovaca (standard type), the death phase started on the third to fifth day, the same or 1 day after the replication peak, with gradual degradation of DNA (Boldis et al 2009; Eremeeva et al 2003). During the 14 days of culturing and 7 days after the peak of replication, there was no observed degradation of DNA for R. helvetica.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

2011

View full text Add to dashboard Cite

Rickettsia helvetica, a spotted fever rickettsia and emerging pathogen with Ixodes ricinus ticks as the main vector, is an agent of human disease and may cause febrile illness as well as meningitis. In three parallel series the isolated standard type of R. helvetica, obtained from a PCR-positive I. ricinus tick, was high-passaged and propagated in a Vero cell line. By using quantitative real-time PCR, the generation time from inoculation to stationary phase of growth was calculated to 20–22 h. In the static cultivation system the stationary phase was observed from the seventh day after inoculation, and there was no observed degradation of R. helvetica DNA during the 14 days studied. Microscopy showed that the organisms invaded the host cells rapidly and were primarily found free in the cytoplasm and only occasionally located in the nucleus. Four days after inoculation some of the host cells were broken and many indifferent stages of cytoplasmic organic decomposition were seen. However the R. helvetica organism did not show any morphologic alterations and the number of organisms was stable after the replication peak which may indicate that R. helvetica is adapted to growth in a Vero cell line and/or that the phase of degradation occurs later than the 14 days studied. The findings differ from what has been reported for other rickettsiae of the spotted fever group and may be of importance for invasiveness and virulence of R. helvetica.

show abstract

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

2011

View full text Add to dashboard Cite

show abstract

“…(about 2 9 10 6 copies per flask) had similar behavior. Partially, qPCR of R. slovaca in L929 cells was already published (Boldiš et al 2009a). These findings suggest that the R. slovaca might be equally accustomed to growth in L929 and Vero cell lines, and suppose useful multiplication of rickettsiae in both cell lines.…”

Section: Discussionmentioning

confidence: 98%

Dermacentor marginatus and Ixodes ricinus ticks versus L929 and Vero cell lines in Rickettsia slovaca life cycle evaluated by quantitative real time PCR

Boldiš

Špitalská

2009

Exp Appl Acarol

View full text Add to dashboard Cite

Ticks transmit many different pathogens to animals, humans and their pets. Rickettsia slovaca, as a member of the spotted-fever-group rickettsiae is an agent of the human disease Tick-borne lymphadenopathy (TIBOLA), also called Dermacentor-borne necrosis erythema and lymphadenopathy (DEBONEL), which occurs from the Mediterranean to central Europe, transmitted by Dermacentor reticulatus and Dermacentor marginatus (Acari: Ixodidae). In this study, quantitative real time PCR was used to characterize the growth of R. slovaca, strain B in static (mammalian L929 and Vero cells without replacement of growth medium) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. Curves of bacterial growth in static cultivations were modeled with exponential, stationary and death phases, whereas in dynamic systems the stationary phase was absent. The highest point of multiplication of R. slovaca was recorded on the 4th day post infection in both cell lines and the rickettsial DNA copy number in L929 and Vero cells at this point was 21 and 27 times greater than rickettsial DNA copy number of inoculum, respectively. In the dynamic system, the highest point of multiplication was on the 21th and 12th day after feeding of ticks and rickettsial DNA copy numbers were 7,482 and 865 times greater than the inoculum in D. marginatus and I. ricinus, respectively. Life cycle of R. slovaca in mammalian cell lines was shorter; supposedly, bacteria destroyed these cells and ticks, especially D. marginatus, were considered a more appropriate environment.

show abstract

“…Examples of such organisms include obligate intracellular pathogens in the Chlamydia and Rickettsia genera (120)(121)(122)(123)(124)(125). These organisms display distinct morphological forms as they transition from various host environments and growth states.…”

Section: As Bacteria Grow Upmentioning

confidence: 99%

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments

Yang

Blair

Salama

2016

Microbiol Mol Biol Rev

234

192

View full text Add to dashboard Cite

SUMMARYBacteria display an abundance of cellular forms and can change shape during their life cycle. Many plausible models regarding the functional significance of cell morphology have emerged. A greater understanding of the genetic programs underpinning morphological variation in diverse bacterial groups, combined with assays of bacteria under conditions that mimic their varied natural environments, from flowing freshwater streams to diverse human body sites, provides new opportunities to probe the functional significance of cell shape. Here we explore shape diversity among bacteria, at the levels of cell geometry, size, and surface appendages (both placement and number), as it relates to survival in diverse environments. Cell shape in most bacteria is determined by the cell wall. A major challenge in this field has been deconvoluting the effects of differences in the chemical properties of the cell wall and the resulting cell shape perturbations on observed fitness changes. Still, such studies have begun to reveal the selective pressures that drive the diverse forms (or cell wall compositions) observed in mammalian pathogens and bacteria more generally, including efficient adherence to biotic and abiotic surfaces, survival under low-nutrient or stressful conditions, evasion of mammalian complement deposition, efficient dispersal through mucous barriers and tissues, and efficient nutrient acquisition.

show abstract

Life cycle ofRickettsia slovacain L929 cell line studied by quantitative real-time PCR and transmission electron microscopy

Cited by 10 publications

References 13 publications

Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

Dermacentor marginatus and Ixodes ricinus ticks versus L929 and Vero cell lines in Rickettsia slovaca life cycle evaluated by quantitative real time PCR

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments

Contact Info

Product

Resources

About