Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

Elfving, Karin; Lukinius, Agneta; Nilsson, Kenneth

doi:10.1007/s10493-011-9508-7

Cited by 17 publications

(22 citation statements)

References 25 publications

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“…Also, the absence of DNA of a pathogen cannot be interpreted as the absence of the infectious agent. Besides the technical detection limits of PCR-based methods, the timing of sample collection after a tick bite and start of an antibiotic treatment, as well as the tissue tropism of the pathogen strongly affect the ability of pathogen detection [ 35 , 36 ]. The latter is corroborated in this study: Only in one out of the 291 patients with an erythema migrans (EM) -a skin infection caused by B .…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Tick-Borne Pathogens in Humans with Tick Bites and Erythema Migrans, in the Netherlands

et al. 2016

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BackgroundTick-borne diseases are the most prevalent vector-borne diseases in Europe. Knowledge on the incidence and clinical presentation of other tick-borne diseases than Lyme borreliosis and tick-borne encephalitis is minimal, despite the high human exposure to these pathogens through tick bites. Using molecular detection techniques, the frequency of tick-borne infections after exposure through tick bites was estimated.MethodsTicks, blood samples and questionnaires on health status were collected from patients that visited their general practitioner with a tick bite or erythema migrans in 2007 and 2008. The presence of several tick-borne pathogens in 314 ticks and 626 blood samples of this cohort were analyzed using PCR-based methods. Using multivariate logistic regression, associations were explored between pathogens detected in blood and self-reported symptoms at enrolment and during a three-month follow-up period.ResultsHalf of the ticks removed from humans tested positive for Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, Borrelia miyamotoi and several Babesia species. Among 92 Borrelia burgdorferi s. l. positive ticks, 33% carried another pathogen from a different genus. In blood of sixteen out of 626 persons with tick bites or erythema migrans, DNA was detected from Candidatus Neoehrlichia mikurensis (n = 7), Anaplasma phagocytophilum (n = 5), Babesia divergens (n = 3), Borrelia miyamotoi (n = 1) and Borrelia burgdorferi s. l. (n = 1). None of these sixteen individuals reported any overt symptoms that would indicate a corresponding illness during the three-month follow-up period. No associations were found between the presence of pathogen DNA in blood and; self-reported symptoms, with pathogen DNA in the corresponding ticks (n = 8), reported tick attachment duration, tick engorgement, or antibiotic treatment at enrolment.ConclusionsBased on molecular detection techniques, the probability of infection with a tick-borne pathogen other than Lyme spirochetes after a tick bite is roughly 2.4%, in the Netherlands. Similarly, among patients with erythema migrans, the probability of a co-infection with another tick-borne pathogen is approximately 2.7%. How often these infections cause disease symptoms or to what extend co-infections affect the course of Lyme borreliosis needs further investigations.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Tick-Borne Pathogens in Humans with Tick Bites and Erythema Migrans, in the Netherlands

et al. 2016

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“…Examples of such organisms include obligate intracellular pathogens in the Chlamydia and Rickettsia genera (120)(121)(122)(123)(124)(125). These organisms display distinct morphological forms as they transition from various host environments and growth states.…”

Section: As Bacteria Grow Upmentioning

confidence: 99%

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments

Yang

Blair

Salama

2016

Microbiol Mol Biol Rev

234

192

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SUMMARYBacteria display an abundance of cellular forms and can change shape during their life cycle. Many plausible models regarding the functional significance of cell morphology have emerged. A greater understanding of the genetic programs underpinning morphological variation in diverse bacterial groups, combined with assays of bacteria under conditions that mimic their varied natural environments, from flowing freshwater streams to diverse human body sites, provides new opportunities to probe the functional significance of cell shape. Here we explore shape diversity among bacteria, at the levels of cell geometry, size, and surface appendages (both placement and number), as it relates to survival in diverse environments. Cell shape in most bacteria is determined by the cell wall. A major challenge in this field has been deconvoluting the effects of differences in the chemical properties of the cell wall and the resulting cell shape perturbations on observed fitness changes. Still, such studies have begun to reveal the selective pressures that drive the diverse forms (or cell wall compositions) observed in mammalian pathogens and bacteria more generally, including efficient adherence to biotic and abiotic surfaces, survival under low-nutrient or stressful conditions, evasion of mammalian complement deposition, efficient dispersal through mucous barriers and tissues, and efficient nutrient acquisition.

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“…Also, it should be noted that the absence of DNA of a pathogen cannot be interpreted as the absence of the infectious agent. Cerar et al [82] and Elfving et al [83] mentioned that besides the limits of PCR-based methods, the time of sample collection after a tick bite and beginning of an antibiotic treatment, or the tissue tropism of the pathogen, strongly affect the ability of pathogen detection. In a study by Jahfari et al [79], only in one of the 291 patients with an erythema migrans -caused by B. burgdorferi s.l.…”

Section: Tick-borne Diseases In Urban and Peri-urban Areas Mean A Rismentioning

confidence: 99%

The role of companion animals in the environmental circulation of tick-borne bacterial pathogens

Skotarczak¹

2018

Ann Agric Environ Med.

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Ticks are known as vectors of a wide range of pathogens of medical and veterinary importance; some of them of zoonotic concern constitute a hazard for the emergence of tick-borne diseases shared between humans and domestic animals and becoming a part of the 'One Health' concept. Canine and feline tick-borne diseases have emerged in recent years, performing an extensive geographic distribution and enlarged global prevalence. The present review focuses on the recent epidemiological studies on the emergence of tick-borne bacterial pathogens in dogs and cats, and the discussion whether pet ownership increases the risk of tick-borne diseases. A lot of data provide confirmation that dogs and cats themselves may substantially contribute to the circulation of the ticks and tick-borne bacterial pathogens in the environment. Molecular diagnostics of tick-borne pathogens infections generates a lot of problems like the choice of molecular methods and molecular markers for the detection of bacterial genomic DNA, but play an important role in the diagnosis of infections. The study provides some insight into molecular diagnostic techniques and new potentially recognized bacterial pathogens of this group. Protecting human and companion animal health from vector-borne infections requires controlling vector populations, containing development of novel, practicable strategies that will limit vectors and transmission of vector-borne disease pathogens.

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Life cycle, growth characteristics and host cell response of Rickettsia helvetica in a Vero cell line

Cited by 17 publications

References 25 publications

Molecular Detection of Tick-Borne Pathogens in Humans with Tick Bites and Erythema Migrans, in the Netherlands

Molecular Detection of Tick-Borne Pathogens in Humans with Tick Bites and Erythema Migrans, in the Netherlands

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments

The role of companion animals in the environmental circulation of tick-borne bacterial pathogens

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